LOCUS       sequence-464715-        2404 bp    DNA     circular SYN 15-APR-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            CDS_mBlueberry2_PartType9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the Genera 
            Xanthobacter and Roseixanthobacter
  JOURNAL   ACS Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806.
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology. 2025. doi: 10.1021/acssynbio.4c00806."
COMMENT     Sequence Label: pXTK089 [CDS_mBlueberry2_PartType9]
FEATURES             Location/Qualifiers
     source          1..2404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             617..1327
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHVEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMFGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGVVTVTQDSSLQDGVFIYKVKLRGTNFPSDGPVMQCRTMGWEAFTERMYPEDGALK
                     SEIKTRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(762..780)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        1382..1473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1474..1542,1543..2334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2338..2377
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaatgg tgagcaaggg cgaggagaat aacatggcca tcatcaagga
      661 gttcatgcgc ttcaaggtgc acgtggaggg ctccgtgaac ggccacgagt tcgagatcga
      721 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      781 gggtggcccc ctgcccttca cctgggacat cctgtcccct cagttcatgt tcggctccaa
      841 ggtctacatt aagcatccag ccgacatccc cgactacttc aagctgtcct tccccgaggg
      901 cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      961 ctcctccctg caggacggcg tgttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1021 ctccgacggc cccgtaatgc agtgtcgtac catgggctgg gaggccttca ctgagcggat
     1081 gtaccccgag gacggcgccc tgaagagcga gatcaagacg aggctgaagc tgaaggacgg
     1141 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1201 cggcgcctac aacgtcgaca tcaagttgga catcgtgtcc cacaacgagg actacaccat
     1261 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     1321 caagtaaatc ttgagaccag agtagcccgc ctaatgagcg ggcttttttt taattcccct
     1381 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1441 taaatgcttc aataatattg aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg
     1501 ctgattccgt tttttgcggc gttttgcctg ccggtgtttg cgcatccgga aaccctggtg
     1561 aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg gctatattga actggatctg
     1621 aacagcggca aaattctgga atcttttcgt ccggaagaac gttttccgat gatgagcacc
     1681 tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca ggaacaactg
     1741 ggccgtcgta ttcattatag ccagaacgat ctggtggaat atagcccggt gaccgaaaaa
     1801 catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat
     1861 aacaccgcgg cgaacctgct gctgacgacc attggcggtc cgaaagaact gaccgcgttt
     1921 ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa
     1981 gcgattccga acgatgaacg tgataccacc atgccggcag caatggcgac caccctgcgt
     2041 aaactgctga cgggtgagct gctgaccctg gcaagccgcc agcaactgat tgattggatg
     2101 gaagcggata aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt
     2161 gcggataaaa gcggtgcggg cgaacgtggc agccgtggca ttattgcggc gctgggcccg
     2221 gatggtaaac cgagccgtat tgtggtgatt tataccaccg gcagccaggc gacgatggat
     2281 gaacgtaacc gtcagattgc ggaaattggc gcgagcctga ttaaacattg gtaaaccgat
     2341 acaattaaag gctccttttg gagccttttt ttttggacga cccttgtcag atcaaaggat
     2401 cttc
//
