LOCUS       sequence-46484-e         804 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA,
            Settleman J
  TITLE     TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers.
  JOURNAL   Cell. 2012 Feb 17;148(4):639-50.
  PUBMED    22341439
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-02-17"; volume: "148(4)"; pages: "639-50"
COMMENT     Sequence Label: pLenti6-BMPR1A(Q233D)-V5
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     94..114
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(151..192)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    245..269
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgnanac
       61 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
      121 ttaattccaa actcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg
      181 gataggctta ccttcgaacc gcgggccctc tagactcgag cggccgccac tgtgctggat
      241 atcaaccact ttgtacaaga aagctgggtc acagattttt acatcttggg attcaaccat
      301 cttggcaagc gtcttcttaa ttctcaatgc tgtgagtctg gaggctggat tgtgggccca
      361 gcattctgac attagcttca aaactgctcg tagacattca tcactgttcc accgattaga
      421 cacaattggc cgcaaacgtt tgacacacac aacctcacgc atatcttcgt atgacggatc
      481 actcggtacc atgttgtaat atggcaattg gtattcttcc acgatccctc ctgtgataca
      541 acgacgagcc atctcccaaa tgattaggcc gaagctgtag atgtcagcca tgatgtaggg
      601 ctggaagtgg tttttgttca ggctttcgtc cagcacttcg ggagccatgt agcgtttggt
      661 gcccaccctg gtattcaagg gcacatcaac ttcatttgtg tcactgttga atttaacagc
      721 aaggcccagg tcagcaatgc agcaactccc atttttcttg atgaggatgt ttttgctctt
      781 taggtctcga tgagcaattg cggg
//
