LOCUS       sequence-465241-        5139 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AiE0128h is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way SW, Oster
            A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, 
            Roth JR, Allen S, Ayala A, Bakken TE, Barcelli T, Barta S, Bendrick 
            J, Bertagnolli D, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, 
            Chakka AB, Chakrabarty R, Chance RK, Chavan S, Departee M, Donadio 
            N, Dotson N, Egdorf T, Gabitto M, Garcia J, Gary A, Gasperini M, 
            Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, 
            Helback O, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, 
            Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, 
            Lee AY, Leibly M, Lenz GH, Liang E, Lusk N, Malone J, Mollenkopf T, 
            Morin E, Newman D, Ng L, Ngo K, Omstead V, Oyama A, Pham T, Pom CA, 
            Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, 
            Sanchez REA, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shulga L, 
            Sigler AR, Siverts LA, Somasundaram S, Stewart K, Szelenyi E, Tieu 
            M, Trader C, van Velthoven CTJ, Walker M, Weed N, Wirthlin M, Wood 
            T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch
            K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan 
            B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting J, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244.
  PUBMED    38915722
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244."
COMMENT     Sequence Label: AiP1995 -
            pAAV-hsA2-AiE0128h-minRho-iCre(R297T...
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    16..127
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   245..385
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   245..374
                     /label=AAV2 ITR
     rep_origin      460..915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(547..566)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     757..778
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(932..951)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1051..1073
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1111..1129)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1197..1301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1302..1370,1371..2162)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1520..1539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2333..2921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2822..2841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2983..3112
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      4057..4066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4069..4089
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgagagatc ttcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
       61 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      121 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
      181 gcaaggggga ggattgggaa gacaatagca ggcatgagat ctcacgtgcg gaccgagcgg
      241 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
      301 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
      361 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
      421 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
      481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
      541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      661 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      781 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
      841 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgggaatttt aacaaaatat
      901 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      961 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     1021 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     1081 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     1141 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1201 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1261 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1321 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1381 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1441 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1501 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1561 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1621 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1681 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1741 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1801 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1861 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1921 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1981 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2041 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2101 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2161 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2221 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2281 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2341 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2401 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2461 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2521 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2581 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2641 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2701 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2761 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2821 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2881 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2941 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtcctgcagg cagctgcgcg
     3001 ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
     3061 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggccgc
     3121 aacgcgttta gaacaatggc tggcccatag taaatgccgt gttagtgtgt tagttgctgt
     3181 tcttccacgt cagaagaggc acagacaaat taccaccagg tggcgctcag agtctgcgga
     3241 ggcatcacaa cagccctgaa tttgaatcct gctctgccac tgcctagttg agacctttta
     3301 ctacctgact agctgtttgt gtattttagg tgtttgtttc atcaaggtca ggggccttac
     3361 ctctcctagt atcatgagga ggatctcaaa gcagggaagg gaagccagct gagggacccc
     3421 tgagaggaga tcaggggata aatactttgc aacagaatcc ccatgggaaa catgacaaaa
     3481 ttagtaacag gaatggagcc tccctcagag gatacatgaa tcgttgagtt gaaaataacc
     3541 acaaggtcat caaatccagc tgccaccaac tgcctgaata ttttcaacaa atttttgaac
     3601 aagcagtcct ccagcctcct ccagctgcat ccagcagctc agtgctccac ctgacagcct
     3661 cttctgatag cgtgaaggct cagaaagccc ctgtggggcc cagcacatcc tggggcaggc
     3721 ctctagtggg cagagggcag catactgggg tctggatcta acacactcct ccatccaggt
     3781 ggtatattta tagccctgtg ctttacagaa aagacttctg atgagatcaa gactttaggc
     3841 tgtttatgtg gcttctttca tgagccaact gaattcgata tcataatcaa ccataggtac
     3901 cgagctcggg attcagccgg gagcttaggg aggggaggtc acttcataag ggcctggggg
     3961 gggagttgga gccacgagtc gtccagccgg agccccgtgt ggctgagctc cggcctcaga
     4021 agcatccccg ggttggatcc agatctttcg aagctagcca ccatggtgcc caagaagaag
     4081 aggaaagtct ccaacctgct gactgtgcac caaaacctgc ctgccctccc tgtggatgcc
     4141 acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca ggccttctct
     4201 gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg gtgcaagctg
     4261 aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct cctgtacctg
     4321 caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct caacatgctg
     4381 cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct ggtgatgagg
     4441 agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct ggcctttgaa
     4501 cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg ccaggacatc
     4561 aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc cgaaattgcc
     4621 agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat ccacattggc
     4681 aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct gggggttacc
     4741 aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa caactacctg
     4801 ttctgccggg tcagaaagaa tggtgtggct gccccttctg ccacctccca actgtccacc
     4861 cgggccctgg aagggatctt tgaggccacc caccgcctga tctatggtgc caaggatgac
     4921 tctgggcaga gatacctggc ctggtctggc cactctgcca gagtgggtgc tgccaccgac
     4981 atggccaggg ctggtgtgtc catccctgaa atcatgcagg ctggtggctg gaccaatgtg
     5041 aacattgtga tgaactacat cagaaacctg gactctgaga ctggggccat ggtgaggctg
     5101 ctcgaagatg gggactgagg cgcgccgaat tcaagcttc
//
