LOCUS       sequence-465805-        7066 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  5' AAVLINK plasmid for CUL7 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Zhonghua Lu Lab
  TITLE     Zhonghua Lu AAV Plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' CUL7
FEATURES             Location/Qualifiers
     source          1..7066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        186..397
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             410..678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             715..741
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     repeat_region   4329..4469
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4329..4458
                     /label=AAV2 ITR
     rep_origin      4544..4999
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4631..4650)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4841..4862
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5016..5035)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5135..5157
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5195..5213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5281..5385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5386..5454,5455..6246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5604..5623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6417..7005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6906..6925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc tgcgatcgct ccggtgcccg
      181 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      241 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      301 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      361 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
      421 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
      481 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
      541 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
      601 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
      661 tctgcgccgt tacagatcca agctgtgacc ggcgcctacc gtacggccac catgtaccct
      721 tatgacgtac ctgactatgc tggatctgtg ggagaactcc gctacaggga attcagggtg
      781 cccctggggc ccggcttaca tgcctatcct gatgagctga tccgccagcg cgtgggccat
      841 gatgggcatc ctgagtacca gatccgttgg ctcatcctgc ggcgtggcga tgagggggac
      901 gggggctctg gccaagtgga ctgcaaggct gagcacatcc tgctgtggat gtccaaggat
      961 gagatctatg ccaactgcca caagatgctg ggcgaggatg gccaggtcat cgggccctcc
     1021 caggagtctg caggggaggt tggggccctg gacaaatctg tgctggagga gatggaaacc
     1081 gatgtgaagt ccctcattca gagagccctt cggcagctgg aggagtgtgt gggcactatc
     1141 cctcctgctc ctctacttca cactgtccac gtgctcagcg cctatgccag cattgagccc
     1201 ctcactggag tattcaagga cccaagggtc ctggacttgc tcatgcacat gttgagtagt
     1261 cctgattatc agattcgctg gagtgcaggc cggatgatac aagccctgtc ctcccatgac
     1321 gctgggaccc ggactcagat ccttctgtca ctgagccaac aagaagccat tgagaaacac
     1381 ctggattttg acagccgctg tgctctgcta gcactgtttg cacaggccac gctctctgaa
     1441 caccccatgt ctttcgaggg cattcagcta ccacaggtcc caggaagggt gctcttctcc
     1501 ctggtgaagc ggtatttgca tgtcacctcg ctcctggatc agctgaacga cagtgctgcg
     1561 gagccaggag cccagaacac ctctgctcct gaggagttga gtggggagag gggtcaactg
     1621 gagctggagt tcagtatggc catgggcacc ctgatctcgg agctggtgca agccatgcgc
     1681 tgggaccagg cctcagacag accaaggagc tcagcacggt cccccggttc catcttccag
     1741 cctcagctgg cagatgtgag cccagggctc cccgctgccc aggctcagcc ctccttcagg
     1801 aggtcaagac gttttcgccc tcgttctgag ttcgcaagtg gcaataccta tgctttgtat
     1861 gtgcgggaca cactgcagcc ggggatgcga gtgcggatgc tggatgatta tgaggagatc
     1921 agtgccgggg atgagggcga gtttcggcag agcaacaacg gtgtgcctcc tgtgcaggta
     1981 ttttgggagt caacaggccg cacctattgg gtgcactggc acatgctgga gatcttgggc
     2041 tttgaggaag acattgagga catggttgag gctgatgagt accaaggggc agtggccagt
     2101 agagtcctgg gtagagccct gcctgcctgg cgctggaggc ccatgacaga actctatgct
     2161 gtgccttatg tgctgcctga ggatgaggac actgaggagt gtgaacacct gaccctggct
     2221 gagtggtggg aactcctctt cttcatcaag aagctggatg gacctgacca tcaggaggtt
     2281 ctccagatcc tccaggagaa cctagatggg gagattctgg atgatgagat cctagctgaa
     2341 ctggccgtgc ccatagaatt ggcccaggac ttgctgctga ctctgccaca gcgactcaat
     2401 gacagtgccc tcagggacct gatcaactgc catgtctaca agaagtatgg gcctgaagcc
     2461 ctagcaggga accaagccta cccatccctt ctagaagccc aagaagatgt cctcctgcta
     2521 gacgcgcagg cccaggctaa ggactcagaa gatgcagcca aagtggaagc aaaagaaccc
     2581 ccatctcaga gtcccaacac tcccctgcag cgtctggtgg agggttatgg tccagctggg
     2641 aaaatcctcc tggatctaga gcaagccctc agctcagagg ggacccagga gaacaaggtc
     2701 aagccactcc tgctgcagct gcagcggcag ccgcagccct tcctggcact gatgcagagc
     2761 ctggacactc cggagactaa caggaccctg cacctgactg tgctgagaat cctgaagcag
     2821 ctggtggact tccccgaggc actgctgctc ccctggcacg aggccgtgga tgcctgcatg
     2881 gcctgcctgc ggtccccaaa cactgatcga gaggtgctcc aggaactgat tttcttcctg
     2941 caccgcctga cctcagtgag cagggactat gccgtggtgc tgaatcagct gggagcaaga
     3001 gacgctatct ccaaggccct ggaaaagcac ctgggaaagc tggagctggc tcaggagctg
     3061 cgggacatgg tgttcaagtg tgagaagcat gcccacctct accgcaaact catcaccaac
     3121 atcctgggag gctgcatcca gatggtgctg ggccagatcg aagaccacag acgaacccac
     3181 cggcccatca acatcccttt ctttgatgtg ttcctcagat acctgtgcca gggctccagt
     3241 gtggaagtga aggaggacaa gtgctgggag aaggtggagg tgtcctccaa cccgcaccgg
     3301 gccagcaagc tgacggacca caaccccaag acctattggg agtccaacgg cagcgccggc
     3361 tcccactaca tcaccctgca catgcgccgg ggcatcctca tcaggcaact gactctgctt
     3421 gtggctagtg aggactcgag ttacatgccg gcccgagtgg tggtgtgcgg gggtgatagc
     3481 actagctctc ttcacacgga actcaactcg gtgaatgtga tgccctctgc cagccgggtg
     3541 atcctcctgg agaacctgac ccgcttctgg cccatcatcc agatccgcat aaagcgctgc
     3601 cagcagggtg gcattgatac gcgcattcgg gggttagaga tcctaggccc caagcccacg
     3661 ttctggccag tgttccggga gcagctctgt cgtcacacac gcctcttcta catggttcgg
     3721 gcacaggcct ggagccagga catggcagag gaccgcagga gcctcctgca cctgagttct
     3781 agactcaacg gtgctctgcg ccaggagcag aattttgctg accgcttcct ccctgatgac
     3841 gaggctgccc aagctctggg caagacctgc tgggaggccc tggtcagccc cgtggtgcag
     3901 aacatcacct cccctgatga ggatggcatt agccccctgg gttggctgct ggaccagtac
     3961 ctggagtgtc aggaagctgt cttcaacccc cagagccgcg gcccagcttt cttctcgcgg
     4021 gtgcgccgtc tcactcacct gctggtgcat gtcgagccct gtgaggcacc ccctcctgtg
     4081 gtggccactc ctcggcccaa aggcagaaac agaagccacg actggagctc cttggctacc
     4141 cggggccttc caagcagcat catgagaaac ctgacgcgct gttggcgggc cgtggtggag
     4201 aagcaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc tattattcgt
     4261 ataggatact ttatacgaag ttattaagtt atggagaccg gtgtggcacg tgcggaccga
     4321 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4381 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4441 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     4501 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     4561 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4621 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4681 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4741 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4801 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4861 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4921 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4981 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5041 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5101 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5161 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5221 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5281 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5341 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5401 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5461 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5521 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5581 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5641 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5701 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5761 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5821 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5881 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5941 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6001 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6061 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6121 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6181 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6241 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6301 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6361 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6421 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6481 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6541 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6601 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6661 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6721 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6781 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6841 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6901 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6961 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7021 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
