LOCUS       sequence-466068-        9687 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A CMV driven human LEF1 followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)
FEATURES             Location/Qualifiers
     source          1..9687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    110..343
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    836..961
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1008..1188
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1189..1415)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1443..1463)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1443..1461)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1482..1498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1482..1498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1495..1517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1506..1522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1530..1536,1537..1554,1555..1560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1575..1596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1713..1730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1884..2472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1964..1983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2643..3434,3435..3503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3266..3285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3504..3608)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3634..4089)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3771..3792)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3983..4002
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4216..4238
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4230..4247
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4231..4247
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4257..4276
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4257..4275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4296..4431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4471..4592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4482..4501)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4536..4555
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4664..4897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4984..5382)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5401..5448)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5462..5961)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5510..5529)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5903..5926
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(5994..6035)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6088..6112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6130..6840)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6210..6233)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6408..6427)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6677..6695
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7237..7293)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8518..8559)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      8559..8568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8575..8599)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8695..8719)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8716..8919)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8745..8765)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8920..9223
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9568..9612)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
       61 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
      121 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
      181 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
      241 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
      301 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
      361 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
      421 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg
      481 tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
      541 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
      601 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
      661 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
      721 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
      781 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
      841 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
      901 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
      961 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
     1021 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     1081 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     1141 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccgt ttatgtatcg
     1201 agctaggcac ttaaatacaa tatctctgca atgcggcaat tcagtggttc gtccaatcca
     1261 tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa
     1321 tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat
     1381 gttgcaagac tacaagagta ttgcataaga ctacattaag cttgcagctc cagcttttgt
     1441 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     1501 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1561 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1621 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1681 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3601 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     3661 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3721 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3781 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3841 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3901 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3961 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4021 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4081 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4141 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4201 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     4261 acgactcact atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta
     4321 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     4381 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     4441 taggggcggg atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca
     4501 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4561 ttgtaaccat tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt
     4621 gcaagttata aatactgaat aataagatga catgaactac tactgctaga gattttccac
     4681 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     4741 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     4801 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc
     4861 agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag
     4921 tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtaccga gctcgaattg
     4981 tgcttagccc tcccacacat aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg
     5041 ctgtccatca ctgtccttca ctatggcttt gatcccagga tgcagatcga gaagcacctg
     5101 tcggcaccgt ccgcaggggc tcaagatgcc cctgttctca tttccgatcg cgacgataca
     5161 agtcaggttg ccagctgccg cagcagcagc agtgcccagc accacgagtt ctgcacaagg
     5221 tcccccagta aaatgatata cattgacacc agtgaagatg cggccgtcgc tagagagagc
     5281 tgcgctggcg acgctgtagt cttcagagat ggggatgctg ttgattgtag ccgttgctct
     5341 ttcaatgagg gtggattctt cttgagacaa aggcttggcc atggtttagt tcctcacctt
     5401 gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac gtgtctagag
     5461 tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg
     5521 tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc
     5581 tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa
     5641 gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca
     5701 tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct
     5761 gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac
     5821 caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc
     5881 cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg
     5941 ggaaaagcgc ctcccctacc cggtaggtac catcgtcatt actaaccggt acgcgtagaa
     6001 tcgagaccga ggagagggtt agggataggc ttaccttcga accgcgggcc ctctagactc
     6061 gagcggccgc cactgtgctg gatatcaacc actttgtaca agaaagctgg gagatctctc
     6121 gagctatcat tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg
     6181 ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttgatgtt
     6241 gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
     6301 ggcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc
     6361 gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
     6421 tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
     6481 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
     6541 gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg
     6601 gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa
     6661 gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
     6721 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
     6781 cttgaagcgc atgaactcct tgatgatggc catgttgtcc tcctcgccct tgctcaccat
     6841 cccccctgcg gttttctgct ggcccttctt accaatcaga gacttgtgga tgtgaggaat
     6901 gactcctcct ccagcaatgg tggccttgat aagcgagtcc agctcctcgt ctccgcggat
     6961 ggccagctgt aaatgtctgg gagtgattcg cttcaccttc agatccttag aagcgtttcc
     7021 agccaactcc agcacctcag cggtgagata ctccaggata gcagcactgt aaaccgcagc
     7081 tgtagctccg acacggccat ggctggtggt tcgtgtcttc aggtgcctgt ggatacgacc
     7141 tacagggaac tgcagaccag ctctctgaga tctggagacg gctttcgtct tggcttttcc
     7201 actgtcttta cctgcttttc cacctgccat cctaggtggc ccgggattct cttcgacatc
     7261 ccctgcttgt ttcaacaggg agaagttagt ggctccggta ccgcagatgt aggcagctgt
     7321 cattcttgga cctgtacctg atgcagattc ctgtagtttc tctctcttcc tcttcttttt
     7381 cttaccataa ttgtctcttg cagaccagcc tggataaagc tgcatatgta gctgtctttc
     7441 tttccgtgct aattcataat atttagcctg ctcttcacgg gagagggcat gccaccttct
     7501 gccaagaatc tggttgatag ctgcactttc ttttagagta cactcagcaa cgacattcgc
     7561 tctcatttct ttcatgtata acataaaagc attcagaggc ttcttaatgt gaggtctttt
     7621 tggctcctgc tcctttctct gttcatgctg aggcttcacg tgcattaggt cactgtcagt
     7681 gtggggatgt tcctgtttga cctgaggtgt tacaatagct ggatgaggga tgccagttgt
     7741 gtggggacca ggaggaccgg gaatcatatg atgggaaaac ctggacatgg aagtgtcgac
     7801 tgacagtgag gatgggtagg gttgcctgaa tccacccgtg atgggatata caggctgacc
     7861 ttgccagcca agaggtgggg tgatctgtcc aacaccaccc ggagacaagg gataaaaagt
     7921 agggatatca ggagctggag gatgtctgga catgccttgt ttggagttga catctgatgg
     7981 gatgtgtgac gggtgtgatc ctggagaaaa gtgctcgtca ctgtaagtga tgaggggggt
     8041 gagaggatgg accgcatggg atggctgcac cacgggcact ttatttgatg ttctcgggat
     8101 gggtggagaa agagatccat ttgacatgta tgggtcgtta ttcatatttg gcatcattat
     8161 gtacccggaa taactcgagt aggagggtcc cttgttgtag aggcctccat ctggatgctt
     8221 tccgtcatcg gggtgttctc tggccttgtc gtggtagggc tcctgagagg tttgtgcttg
     8281 tctggccacc tcgtgtccgt tgctggccgg gatgatttca gactcgttca ccaaggaaga
     8341 cttgatgtca gctaaatcgc cttcctcttc gggatgactg atctcggcga agatcttttc
     8401 cttctgagga tcgccctcgt ccttgaaggg gatcatctcg tccgtggcgc agagttccgg
     8461 gtcccccccg ccgccgccac ctcctccgga gagttggggc ataccaccag caccacccgt
     8521 agaatcgaga ccgaggagag ggttagggat aggcttaccc atggtggcgg atccagcctg
     8581 cttttttgta caaacttgtt gatatctgca gaattccacc acactggact agtggatcct
     8641 ctagagtcgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg
     8701 acggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     8761 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     8821 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     8881 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     8941 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     9001 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     9061 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     9121 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     9181 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact cccaagctta
     9241 tcgcagaccc agtcgctagc gttaacgtcg acatcgcaga cccagtcgct agcgttaacg
     9301 tcgacatcga taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc
     9361 tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt
     9421 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
     9481 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
     9541 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
     9601 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
     9661 gtaattgtta atttctctgt cccactc
//
