LOCUS       sequence-466535-        8659 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of WT SRP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Xi X, Liu L, Tuano N, Tailhades J, Mouradov D, Sieber O, Cryle M, 
            Segelov E, Rosenbluh J
  TITLE     SRP19 and the protein secretion machinery is a targetable 
            vulnerability in cancers with APC loss
  JOURNAL   bioRxiv 2024.01.30.578083; doi: 
            https://doi.org/10.1101/2024.01.30.578083
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.30.578083; doi: https://doi.org/10.1101/2024.01.30.578083"
COMMENT     Sequence Label: SRP19 in pLX311
FEATURES             Location/Qualifiers
     source          1..8659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1754..1778)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1786..1827
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1878..2466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1931..1951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2349..2360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2538..2771
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2849..2970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2886..2905)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2940..2959
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3010..3145
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3165..3184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3166..3184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3194..3211)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3194..3210)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3203..3225)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3352..3807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3439..3458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3649..3670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3833..3937
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3938..4006,4007..4798)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4156..4175)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4969..5557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5458..5477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5711..5728
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5845..5866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5881..5886,5887..5904,5905..5911)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5919..5935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5924..5946
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5943..5959
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5943..5959
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5978..5998
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5980..5998
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6026..6252
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6253..6433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6480..6605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7098..7331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7516..7560
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7791..8001
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7813..8009
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7860..7995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8031..8426
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8500..8617
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agttggcatg
     1321 gcttgcgctg ccgcgcggtc cccggccgac caggacaggt ttatttgtat ctatcctgct
     1381 tatttaaata ataagaagac catcgcagag ggaaggcgaa tccccataag taaggctgtt
     1441 gaaaatccta cagctacaga gattcaagat gtatgttcag cagttggact taacgtattt
     1501 cttgagaaaa ataaaatgta ctctagagaa tggaatcgtg atgtccaata cagaggcaga
     1561 gtccgggtcc agctcaaaca ggaagatggg agcctctgcc ttgtacagtt cccatcacgt
     1621 aagtcagtaa tgttgtatgc agcagaaatg atacctaaac taaaaacaag gacacaaaaa
     1681 acaggaggtg ctgaccaaag tcttcaacaa ggagagggaa gtaaaaaagg gaaaggaaag
     1741 aaaaagaaga gacccccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1801 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtaccggtta agtcgacaat
     1861 caacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     1921 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     1981 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2041 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2101 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2161 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2221 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2281 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2341 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2401 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2461 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     2521 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     2581 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2641 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     2701 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     2761 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     2821 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     2881 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2941 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     3001 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3061 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3121 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     3181 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3241 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3301 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     3361 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3421 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3481 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3541 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3661 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3721 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3781 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     3841 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3901 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3961 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4021 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4081 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4141 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4201 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4261 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4321 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4381 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4441 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4501 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4561 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4621 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4681 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4741 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4801 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4861 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4921 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4981 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5041 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5101 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5161 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5221 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5281 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5341 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5401 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5461 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5521 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5581 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5641 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5701 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5761 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5821 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5881 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5941 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     6001 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     6061 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     6121 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     6181 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     6241 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6301 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6361 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6421 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     6481 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     6541 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     6601 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     6661 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     6721 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     6781 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     6841 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     6901 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     6961 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7021 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7081 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7141 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     7201 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7261 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     7321 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     7381 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     7441 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     7501 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     7561 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     7621 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     7681 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat
     7741 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7801 gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca
     7861 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7921 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7981 gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc atggccaagc
     8041 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     8101 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     8161 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     8221 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     8281 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     8341 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     8401 tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtaaggcgcg ccgttaacga
     8461 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     8521 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     8581 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     8641 agatccactt tggcgccgg
//
