LOCUS       sequence-466753-        9239 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N EBNA3B
FEATURES             Location/Qualifiers
     source          1..9239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1418..1427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1427..1450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1463..1489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1505..1529
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4341..4365)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4378..4945
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4395..4947
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4539..4556)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4766..4785
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4959..5558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4959..4978)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5455..5475
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5634..6148
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6310..6326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6334..6340,6341..6358,6359..6364))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6379..6400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6517..6534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6688..7276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6768..6787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7447..8238,8239..8307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8070..8089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8308..8412)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8480..8498
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8536..8558)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8658..8677
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8871..8893
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9021..9040
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctgcc accatggact acaaggatga
     1441 cgatgacaag ctcgatggag gatacccata cgatgttcca gattacgctg ctcgaggtta
     1501 tcaaacaagt ttgtacaaaa aagttggcat gaagaaagcg tggctcagca gagcacagca
     1561 agccgatgcc gggggggcat ctggctccga ggacccacca gattatggag atcaaggtaa
     1621 tgtgacacag gtgggatctg agcctatttc acctgagatt ggcccctttg aactctctgc
     1681 ggccagtgag gatgatcctc aatctgggcc agtggaagag aatttagatg ccgctgcaag
     1741 agaggaagag gaacctcatg agcaggagca caatggtggt gacgatccct tggatgtcca
     1801 tactcgccag cctagatttg tggatgtgaa cccaacgcag gctccagtga tccaactagt
     1861 ccacgctgtc tatgattcca tgttgcaatc ggacctccgg cccctaggca gtttattcct
     1921 tgagcaaaac ctgaacatcg aagaatttat atggatgtgc atgaccgtgc gtcacagatg
     1981 tcaggccatc agaaaaaaac cattaccaat tgttaagcag aggcgttgga agctcctgtc
     2041 atcttgcaga tcctggcgta tgggttaccg cacgcataac ctcaaagtaa acagttttga
     2101 gtcagggggg gacaatgtcc acccggtcct tgtgactgct acgctaggat gtgatgaggg
     2161 cacgcggcat gcaacaacgt acagtgctgg cattgtacag ataccacgaa tatcagacca
     2221 aaaccaaaag atcgaaacag ccttcctgat ggcacgtcgt gctaggtcac tttcggcaga
     2281 aagatatact ttgttctttg atttagtatc ctccggaaac accctgtatg ctatatggat
     2341 tgggctgggc acgaaaaacc gagtttcatt tattgagttt gtaggatggt tatgcaagaa
     2401 ggaccacact catatacgcg aatggttccg ccagtgcacc gggagaccca aagcagccaa
     2461 gccatggtta agagcgcatc ctgtcgccat tccttatgat gatccgttaa caaacgagga
     2521 gattgatctg gcctatgccc gcgggcaggc catgaatatt gaggctccta gactgccaga
     2581 tgatcctata attgttgagg atgacgacga aagtgaggaa attgaagctg aaagcgacga
     2641 ggaggaagac aagagtggaa tggaatctct taaaaatata ccgcaaacac tgccgtacaa
     2701 tccaacagta tacggcaggc ccgcggtgtt tgaccgaaag tcagatgcaa aatcaaccaa
     2761 aaaatgcagg gccatagtaa ctgactttag tgtaatcaag gccattgaag aggaacacag
     2821 aaagaagaag gcagccagaa cagagcagcc aagagccacg cctgaatccc aggcccccac
     2881 agtggtcctc cagcgaccac ccacgcagca agagcctggc cccgtcggcc cactgagtgt
     2941 ccaggctcgg ctggagccat ggcaaccttt gcctgggccc caagtgacag cagttctact
     3001 tcacgaagaa tccatgcagg gtgtccaagt acatggttcg atgctagacc ttcttgaaaa
     3061 agacgatgaa gtcatggagc agagggttat ggcaacccta ctgccaccag taccacaaca
     3121 gccccgggct ggcagaagag gcccttgtgt cttcaccggt gacctaggca tagagagtga
     3181 tgagcccgct tcgacagagc cggttcatga tcagctactg cctgccccag gacctgaccc
     3241 tcttgaaatc caaccactaa cgtcccccac cacgtctcaa cttagcagtt cggcaccaag
     3301 ctgcgcacaa actccatggc cggtggttca gccaagtcag actccagatg acccaacgaa
     3361 acagtcccgg ccaccggaaa cagctgcccc acgccagtgg ccaatgcccc tgcgacctat
     3421 ccctatgcgc cccttgcgga tgcagccaat cccatttaat catccagtgg gacccactcc
     3481 ccatcagaca cctcaagtgg aaataacacc atataagccc acttgggctc agatagggca
     3541 cattccatat cagcctacac caacgggtcc tgctaccatg ctgttgcgcc agtgggcacc
     3601 cgccaccatg cagacaccac cgagagcgcc cactcccatg tcaccacctg aggtgccacc
     3661 cgttccccgg cagaggcctc ggggggcgcc cactcccacg ccacctcctc aggtgccgcc
     3721 cgttccccgg cagaggcctc ggggggcgcc cactcccacg ccacctcctc aggtgctgcc
     3781 cactcccatg cagctggcac taagggctcc tgctggtcag caggggccga caaagcaaat
     3841 tttgcgccaa ttgttaacgg ggggcgtcaa gaaagggaga ccatcactta agttacaggc
     3901 cgcccttgag cgtcaagccg ctgcgggctg gcagccttca ccagggtccg gcaccagtga
     3961 caagattgtg caggcgccta ttttctaccc acccgttttg cagcccatac aggttatggg
     4021 gcaagggggt tccccaacgg ccatggccgc ctcagcggtg acacaggcac ccacggaata
     4081 taccagggaa aggaggggag tggggcctat gcctcccacc gatattccgc cgtctaaacg
     4141 agcgaagatc gaggcctata cagagcccga gatgccgcac gggggggcct cgcactctcc
     4201 cgtcgttatc ttggagaatg tcggccaggg gcaacagcag actctggagt gcggaggaac
     4261 tgctaaacag gaaagggaca tgttggggct gggggacatt gcagtttctt ccccttcctc
     4321 ttctgaaaca tcgaacgatg agccaacttt cttgtacaaa gtggttcgat aacgaattcc
     4381 gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     4441 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4501 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4561 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4621 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4681 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4741 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4801 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4861 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4921 tttcctttga aaaacacgat gataatatgg ccacaaccat gaccgagtac aagcccacgg
     4981 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     5041 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     5101 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     5161 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     5221 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     5281 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     5341 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     5401 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     5461 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     5521 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatg catcgatgtc gacctgcagc
     5581 caagcttatc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     5641 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     5701 tacataactg agaatagaga agttcagatc aaggttagga acagagagac agcagaatat
     5761 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     5821 ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg
     5881 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     5941 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     6001 cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtgtatcc aataaaccct
     6061 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     6121 tgactacccg tcagcggggg tctttcatgg gtaacagttt cttgaagttg gagaacaaca
     6181 ttctgagggt aggagtcgaa tattaagtaa tcctgactca attagccact gttttgaatc
     6241 cacatactcc aatactcctg aaatagttca ttatggacag cgcagaaaga gctggggaga
     6301 attgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6361 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7441 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7501 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7561 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7621 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7681 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7741 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7801 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7861 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7921 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7981 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8041 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8101 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8161 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8221 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8281 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8341 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8401 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     8461 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     8521 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     8581 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     8641 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     8701 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     8761 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     8821 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     8881 acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     8941 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     9001 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     9061 gcgccggtga tgccggccac gatgcgtccg gcgtagaggc gattagtcca atttgttaaa
     9121 gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct
     9181 atagagtacg agccatagat aaaataaaag attttattta gtctccagaa aaagggggg
//
