LOCUS       sequence-466775-       11320 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Inducible expression of Tau-mCherry-RING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Miller LVC, Papa G, Vaysburd M, Cheng S, Sweeney PW, Smith A, Franco
            C, Katsinelos T, Huang M, Sanford SAI, Benn J, Farnsworth J, 
            Higginson K, Joyner H, McEwan WA, James LC
  TITLE     Co-opting templated aggregation to degrade pathogenic tau assemblies
            and improve motor function.
  JOURNAL   Cell. 2024 Oct 17;187(21):5967-5980.e17. doi: 
            10.1016/j.cell.2024.08.024. Epub 2024 Sep 13.
  PUBMED    39276772
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.08.024"; journalName: "Cell"; date: 
            "2024-10-17- 17"; volume: "187"; issue: "21"; pages: "5967-5980.e17"
COMMENT     Sequence Label: pLVX-Tau-mCherry-RING
FEATURES             Location/Qualifiers
     source          1..11320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    168..302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        350..454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(455..523,524..1315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(673..692)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1486..2074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1975..1994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2228..2245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2362..2383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2398..2403,2404..2421,2422..2428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2436..2452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2441..2463
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2460..2476
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2460..2476
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2604..3237
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3444..4032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3550..3561
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3959..3979
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4046..4645)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4129..4149)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4626..4645
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4654..4983)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4668..4803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(4994..5740)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(5759..6269)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5822..5840)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        6286..6653
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    6294..6312
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6330..6348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6366..6384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6402..6420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6438..6456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6474..6492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6510..6528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    9000..9134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    9178..9293
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(9556..9600)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9785..10018
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10515..10640
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10687..11320
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca
       61 gaggaatcac aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat
      121 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
      181 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      241 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      301 ttgatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      421 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      481 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      541 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      601 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      661 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      721 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      781 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      841 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      901 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
      961 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1021 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1081 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1141 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1201 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1261 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1321 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1381 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1441 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1501 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1561 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1621 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1681 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1741 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1801 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1861 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1921 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1981 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2041 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2101 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2161 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2221 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2281 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2341 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2401 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2461 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtaaacgcta
     2521 gcaatgtcaa ggcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat
     2581 aataagatga catgaactac tactgctaga gattttccac actgactaaa agggtctgag
     2641 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     2701 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     2761 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     2821 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     2881 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     2941 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     3001 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     3061 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     3121 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     3181 ggtagatcca cagatcaagg atatcttgtc ttcgttggga gtgaattagc ccttccagtc
     3241 ccctcttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     3301 aaggtacctg aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct tctagccagg
     3361 cacaatcagc attggtagct gctgtattgc tacttgtgat tgctccatgt ttttctaggt
     3421 ctcgactgca gaattaattc caggcgggga ggcggcccaa agggagatcc gactcgtctg
     3481 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     3541 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     3601 ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt
     3661 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     3721 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     3781 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     3841 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     3901 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     3961 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
     4021 cagaggttga ttgttccaga cgcgttcagg caccgggctt gcgggtcatg caccaggtgc
     4081 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
     4141 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct
     4201 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc
     4261 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca
     4321 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg
     4381 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt
     4441 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca
     4501 gctcggtgac ccgctcgatg tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt
     4561 cggcgaacgc ggcggcgagg gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc
     4621 gcaccgtggg cttgtactcg gtcatggtcg cgttttgcaa aagcctaggc ctccaaaaaa
     4681 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     4741 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     4801 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     4861 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     4921 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     4981 cacattcgcg cgtttacccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
     5041 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
     5101 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
     5161 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
     5221 ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
     5281 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
     5341 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
     5401 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc
     5461 gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg
     5521 actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg
     5581 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
     5641 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca
     5701 gagcagagtt tatgactttg ctcttgtcca gtctagacat ggtaattcga tgatccccct
     5761 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
     5821 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
     5881 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
     5941 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
     6001 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
     6061 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
     6121 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
     6181 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
     6241 tgccttggaa aaggcgcaac cccaaccccg tggaattatc acctcgagtt tactccctat
     6301 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     6361 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     6421 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     6481 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt
     6541 tgcttatgta aaccagggcg cctataaaag agtgctgatt ttttgagtaa acttcaattc
     6601 cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt aaagaattca
     6661 ccatggctga gccccgccag gagttcgaag tgatggaaga tcacgctggg acgtacgggt
     6721 tgggggacag gaaagatcag gggggctaca ccatgcacca agaccaagag ggtgacacgg
     6781 acgctggcct gaaagctgaa gaagcaggca ttggagacac ccccagcctg gaagacgaag
     6841 ctgctggtca cgtgacccaa gctcgcatgg tcagtaaaag caaagacggg actggaagcg
     6901 atgacaaaaa agccaagggg gctgatggta aaacgaagat cgccacaccg cggggagcag
     6961 cccctccagg ccagaagggc caggccaacg ccaccaggat tccagcaaaa accccgcccg
     7021 ctccaaagac accacccagc tctggtgaac ctccaaaatc aggggatcgc agcggctaca
     7081 gcagccccgg ctccccaggc actcccggca gccgctcccg caccccgtcc cttccaaccc
     7141 cacccacccg ggagcccaag aaggtggcag tggtccgtac tccacccaag tcgccgtctt
     7201 ccgccaagag ccgcctgcag acagcccccg tgcccatgcc agacctgaag aatgtcaagt
     7261 ccaagatcgg ctccactgag aacctgaagc accagccggg aggcgggaag gtgcagataa
     7321 ttaataagaa gctggatctt agcaacgtcc agtccaagtg tggctcaaag gataatatca
     7381 aacacgtcag tggaggcggc agtgtgcaaa tagtctacaa accagttgac ctgagcaagg
     7441 tgacctccaa gtgtggctca ttaggcaaca tccatcataa accaggaggt ggccaggtgg
     7501 aagtaaaatc tgagaagctt gacttcaagg acagagtcca gtcgaagatt gggtccctgg
     7561 acaatatcac ccacgtccct ggcggaggaa ataaaaagat tgaaacccac aagctgacct
     7621 tccgcgagaa cgccaaagcc aagacagacc acggggcgga gatcgtgtac aagtcgccag
     7681 tggtgtctgg ggacacgtct ccacggcatc tcagcaatgt ctcctccacc ggcagcatcg
     7741 acatggtaga ctcgccccag ctcgccacgc tagctgacga ggtgtctgcc tccctggcca
     7801 agcagggttt ggagttcggc ggaggtggct ccatggttag caagggcgag gaagacaata
     7861 tggcaataat taaagaattt atgcgcttca aagtacacat ggagggatca gttaatgggc
     7921 atgagttcga gatagaaggg gagggcgaag ggcgacccta cgaagggacg caaactgcta
     7981 aacttaaagt cacgaagggg ggtcccctgc cttttgcgtg ggatattctg agtccacagt
     8041 tcatgtacgg ctctaaggcg tacgtgaagc atcccgccga catacccgat tacctgaaac
     8101 tgtcattccc cgagggcttt aagtgggaga gggtaatgaa ttttgaagat ggaggggtag
     8161 tcacagttac tcaggactct tctcttcaag atggcgaatt tatatataaa gtgaagctca
     8221 gggggactaa cttcccaagc gatgggccgg ttatgcagaa gaagacgatg ggatgggaag
     8281 catcttccga gcggatgtac cccgaagacg gcgctctcaa gggggaaata aaacaacgac
     8341 tgaaacttaa agatggaggt cactatgacg ctgaggttaa gacgacgtac aaggcaaaaa
     8401 agcccgtaca gcttcctggt gcctataatg taaacataaa acttgatatt acaagccata
     8461 acgaagacta taccatagta gagcagtatg agcgggccga agggcgacac agtaccgggg
     8521 ggatggatga gctgtacaaa ggctccggag gtggctcagg catggcttca gcagcacgct
     8581 tgacaatgat gtgggaggag gtcacatgcc ctatctgcct ggaccccttc gtggagcctg
     8641 tgagcatcga gtgtggccac agcttctgcc aggaatgcat ctctcaggtt gggaaaggtg
     8701 ggggcagcgt ctgtcctgtg tgccggcagc gctttctgct caagaatctc cggcccaatc
     8761 gacagctagc caacatggtg aacaacctta aagaaatcag ccaggaggcc agagagtaag
     8821 gatccagacc acctcccctg cgagctaagc tggacagcca atgacgggta agagagtgac
     8881 atttttcact aacctaagac aggagggccg tcagagctac tgcctaatcc aaagacgggt
     8941 aaaagtgata aaaatgtatc actccaacct aagacaggcg cagcttccga gggatttgag
     9001 atccagacat gataagatac attgatgagt ttggacaaac caaaactaga atgcagtgaa
     9061 aaaaatgcct tatttgtgaa atttgtgatg ctattgcctt atttgtaacc attataagct
     9121 gcaataaaca agttaagtcg ataaactgga tctctgctgt ccctgtaata aacccgaaaa
     9181 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     9241 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaggcgata
     9301 ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
     9361 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
     9421 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
     9481 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
     9541 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
     9601 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
     9661 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
     9721 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
     9781 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
     9841 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
     9901 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
     9961 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
    10021 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
    10081 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
    10141 atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt ggtcttactt
    10201 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
    10261 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
    10321 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
    10381 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
    10441 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
    10501 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
    10561 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
    10621 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
    10681 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
    10741 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
    10801 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
    10861 gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc
    10921 ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc cttgtagcaa
    10981 gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac gtgatgaaat
    11041 gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc catcccatgc
    11101 aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc taccttatct
    11161 ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct gacccctggc
    11221 cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat ccacagatca
    11281 aggatatctt gtcttctttg ggagtgaatt agcccttcca
//
