LOCUS       sequence-466790-        8775 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses SpRY-Tad-CBE(ACC3) in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  TITLE     Weixin Tang Lab Plasmids 2025
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SpRY-Tad-CBE(ACC3)
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(213..1004,1005..1073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     836..855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1074..1178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1257..1276)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1448..1827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1828..2031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1982..2002
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2073..2092
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2073..2091
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2141..2161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2756..6856
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6887..7135
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7166..7414
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7457..7477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7486..7503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7526..7543)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7532..7756
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7827..7843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7827..7843)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7840..7862)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7851..7867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7875..7881,7882..7899,7900..7905))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7920..7941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8058..8075)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8229..8775,1..42))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8309..8328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcggctacca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca
     1261 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     1321 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     1381 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     1441 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1501 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1561 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1621 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1681 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1741 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1801 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     1861 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     1921 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     1981 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     2041 aaccgtcaga tccgctagag atccgcggcc gctaatacga ctcactatag ggagagccgc
     2101 caccatgaaa cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt
     2161 ctctgaagtc gagtttagcc acgagtattg gatgaggcac gcactgaccc tggcaaagcg
     2221 agcacgggat gaaacgacgt ggcccgtggg cgccgtgctg gtgctcaaca atagagtgat
     2281 cggagaggga tggccgaagt gtcgtgatca gcacgaccct accgcacacg cagagatcat
     2341 ggcactgagg cagggaggcc tggtcatgca gaattaccgc ctgtacgatg ccaccctgta
     2401 ttcgacactc gagccatgcg tgatgtgcgc aggagcaatg atccacagca ggatcggaag
     2461 agtggtgttc ggagtgaagc atcatactgg gggcgcagta ggctccctga tgaatgttct
     2521 gcactacccc ggcataaagc accgggtgga gatcacagag ggaatcctgg cagacgagtg
     2581 cgccgccctg ctgtgccgtt tctttagaat gccgagacgg gtgttcaagg cccagaagaa
     2641 ggcacagagc tccaccgact ctggaggatc tagcggaggc tcctctggct ctgagacacc
     2701 tggcacaagc gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcagacaa
     2761 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
     2821 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     2881 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg agagaacccg
     2941 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     3001 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     3061 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     3121 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     3181 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     3241 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     3301 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     3361 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     3421 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     3481 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     3541 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     3601 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     3661 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     3721 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     3781 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     3841 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     3901 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     3961 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     4021 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     4081 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     4141 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     4201 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     4261 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     4321 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     4381 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     4441 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     4501 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     4561 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     4621 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     4681 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     4741 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     4801 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     4861 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     4921 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     4981 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     5041 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     5101 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     5161 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     5221 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc
     5281 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     5341 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     5401 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     5461 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     5521 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     5581 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     5641 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     5701 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     5761 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     5821 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     5881 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     5941 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     6001 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     6061 cggcttcagc aaagagtcta tcagacccaa gaggaacagc gataagctga tcgccagaaa
     6121 gaaggactgg gaccctaaga agtacggcgg cttcctgtgg cccaccgtgg cctattctgt
     6181 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     6241 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     6301 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     6361 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccaagcagc tgcagaaggg
     6421 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     6481 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     6541 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     6601 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     6661 agagcaggcc gagaatatca tccacctgtt taccctgacc agactgggag cccctagagc
     6721 cttcaagtac tttgacacca ccatcgaccc caagcagtac agaagcacca aagaggtgct
     6781 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     6841 tcagctggga ggtgacagcg gcgggagcgg cgggagcggg gggagcacta atctgagcga
     6901 catcattgag aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc
     6961 tgaggaggtg gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc
     7021 ctacgacgag tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa
     7081 gccttgggcc ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgagcgg
     7141 aggatccgga ggatctggag gcagcaccaa cctgtctgac atcatcgaga aggagacagg
     7201 caagcagctg gtcatccagg agagcatcct gatgctgccc gaagaagtcg aagaagtgat
     7261 cggaaacaag cctgagagcg atatcctggt ccataccgcc tacgacgaga gtaccgacga
     7321 aaatgtgatg ctgctgacat ccgacgcccc agagtataag ccctgggctc tggtcatcca
     7381 ggattccaac ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa gaaccgccga
     7441 cggcagcgaa ttcgagccca agaagaagag gaaagtctaa ccggtcatca tcaccatcac
     7501 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7561 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7621 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7681 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7741 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc
     7801 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7861 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa
     7921 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7981 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8041 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8101 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8161 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8221 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8281 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8341 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8401 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8461 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8521 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8581 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8641 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8701 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8761 agcggtggtt ttttt
//
