LOCUS       sequence-466811-        8775 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses Tad-CBE(GCT2) in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  TITLE     Weixin Tang Lab Plasmids 2025
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Tad-CBE(GCT2)
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(78..869,870..938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     701..720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(939..1043)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1122..1141)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1313..1692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1693..1896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1847..1867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1938..1957
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1938..1956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2006..2026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2621..2644,2645..2647,2648..6721)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2621..6721
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6752..7000
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7031..7279
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7322..7342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7351..7368
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7391..7408)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7397..7621
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7692..7708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7692..7708)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7705..7727)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7716..7732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7740..7746,7747..7764,7765..7770))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7785..7806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7923..7940)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8094..8682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8174..8193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
       61 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      121 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      181 ggcttaccat ctggccccag tgctgcaatg ataccgcggc taccacgctc accggctcca
      241 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      301 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      361 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      421 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      481 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
      541 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
      601 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      661 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
      721 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      781 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      841 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      901 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
      961 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1021 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     1081 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc
     1141 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     1201 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     1261 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     1321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     1381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     1441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     1501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     1561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     1621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     1681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     1741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     1801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     1861 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
     1921 tagagatccg cggccgctaa tacgactcac tatagggaga gccgccacca tgaaacggac
     1981 agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtctctg aagtcgagtt
     2041 tagccacgag tattggatga ggcacgcact gaccctggca aagcgagcac gggatgaaaa
     2101 tggggcgccc gtgggcgccg tgctggtgct caacaataga gtgatcggag agggatggcc
     2161 taagttgaat acgatgcacg accctaccgc acacgcagag atcatggcac tgaggcaggg
     2221 aggcctggtc atgcagaatt accgcctgta cgatgccacc ctgtattcga cactcgagcc
     2281 atgcgtgatg tgcgcaggag caatgatcca cagcaggatc ggaagagtgg tgttcggagt
     2341 gaggtatgcg aagtttggcg cagtaggctc cctgatgaat gtgctgcact accccggcat
     2401 aaagcaccgg gtggagatca cagagggaat cctggcagac gagtgcgccg ccctgctgtg
     2461 ccgtttcttt agaatgccga gacgggtgtt caaggcccag aagaaggcac agagctccac
     2521 cgactctgga ggatctagcg gaggatcctc tggcagcgag acaccaggaa caagcgagtc
     2581 agcaacacca gagagcagtg gcggcagcag cggcggcagc gacaagaagt acagcatcgg
     2641 cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     2701 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     2761 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     2821 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     2881 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     2941 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     3001 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     3061 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     3121 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     3181 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     3241 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     3301 gctgcccggc gaaaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     3361 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     3421 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     3481 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     3541 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     3601 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     3661 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     3721 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     3781 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     3841 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     3901 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     3961 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     4021 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     4081 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     4141 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     4201 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     4261 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     4321 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     4381 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     4441 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     4501 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     4561 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     4621 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     4681 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     4741 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     4801 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     4861 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     4921 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     4981 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     5041 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     5101 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     5161 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     5221 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     5281 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     5341 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     5401 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     5461 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     5521 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     5581 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     5641 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     5701 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     5761 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     5821 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     5881 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     5941 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     6001 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     6061 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     6121 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     6181 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     6241 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     6301 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     6361 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     6421 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     6481 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     6541 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     6601 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     6661 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggtga
     6721 cagcggcggg agcggcggga gcggggggag cactaatctg agcgacatca ttgagaagga
     6781 gactgggaaa cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga
     6841 agtgatcggc aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac
     6901 agatgagaat gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt
     6961 catccaggat tctaacggcg agaataagat caagatgctg agcggaggat ccggaggatc
     7021 tggaggcagc accaacctgt ctgacatcat cgagaaggag acaggcaagc agctggtcat
     7081 ccaggagagc atcctgatgc tgcccgaaga agtcgaagaa gtgatcggaa acaagcctga
     7141 gagcgatatc ctggtccata ccgcctacga cgagagtacc gacgaaaatg tgatgctgct
     7201 gacatccgac gccccagagt ataagccctg ggctctggtc atccaggatt ccaacggaga
     7261 gaacaaaatc aaaatgctgt ctggcggctc aaaaagaacc gccgacggca gcgaattcga
     7321 gcccaagaag aagaggaaag tctaaccggt catcatcacc atcaccattg agtttaaacc
     7381 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7441 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7501 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7561 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     7621 gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag ctagagcttg
     7681 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7741 aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc
     7801 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7861 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7921 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7981 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8041 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8101 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8161 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8221 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8281 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8341 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8401 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8461 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8521 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8581 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8641 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8701 tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8761 ttatcaaaaa ggatc
//
