LOCUS       sequence-466905-        9949 bp    DNA     circular SYN 10-JUN-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1394
FEATURES             Location/Qualifiers
     source          1..9949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    235..468
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    961..1086
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1133..1313
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1314..1540)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1568..1588)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1568..1586)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1607..1623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1607..1623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1620..1642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1631..1647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1655..1661,1662..1679,1680..1685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1700..1721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1838..1855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2009..2597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2089..2108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2768..3559,3560..3628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3391..3410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3629..3733)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3759..4214)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3896..3917)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4108..4127
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4341..4363
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4355..4372
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4356..4372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4382..4401
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4382..4400
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4421..4556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4596..4717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4607..4626)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4661..4680
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4795..5028
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5100..5688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5206..5217
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5615..5635
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(7396..8574)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7405..8343
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7428..7448)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8712..8829
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8872..9474)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8958..8978)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9455..9474
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(9506..9702)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(9514..9724)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9520..9655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
       61 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
      121 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
      181 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
      241 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
      301 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
      361 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
      421 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
      481 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
      541 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
      601 ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
      661 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
      721 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
      781 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
      841 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
      901 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
      961 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     1021 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     1081 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     1141 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     1201 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     1261 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg
     1321 tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca
     1381 atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc
     1441 accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt
     1501 accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct
     1561 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     1621 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3361 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3721 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     3781 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3841 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3901 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3961 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     4021 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     4081 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     4141 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     4201 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4261 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     4321 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     4381 gtaatacgac tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct
     4441 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     4501 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     4561 ggagttaggg gcgggatagc tagagccaga catgataaga tacattgatg agtttggaca
     4621 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     4681 tttatttgta accattataa gctgcaataa acaagttcct ctcactctct gatattcatt
     4741 tctttgcaag ttataaatac tgaataataa gatgacatga actactatac gtactgctag
     4801 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     4861 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     4921 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     4981 aagcaaaaag cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt
     5041 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaagtcgacg
     5101 cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag
     5161 aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg
     5221 gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg
     5281 aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc
     5341 tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa
     5401 agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc
     5461 gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata
     5521 aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat
     5581 agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt
     5641 aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacttaacg
     5701 cgttatggac ccttcaatgg ccggtggtcg tcctttccac aagatatata aagccaagaa
     5761 atcgaaatac tttcaagtta cggactagtt cattactaat gagtatgaat tctctggtgt
     5821 ctaacaaggt gcgacttttg gctgaaagcc ttgccacact caatacagtg atagggcttc
     5881 tcacctgtat gttttctcat atgaagggta agagatgaga tttgagagaa ggcttttcca
     5941 catttactac agtcgaaggg tttctcacct gtatgtcctc gtatatgtat agtaagggat
     6001 gagctttggg agaaggcttt tccacattta ttacattcat agggtttttc acctgtatgg
     6061 cctctcatgt gcacaataag ggaagttctt tgagagaagg ctttcccaca ttcattacat
     6121 acatagggct tctcacctgt gtgacttctc atatgtaaat taagcagtga gcactgagag
     6181 aaggctttac cacatttatc acattcataa ggcttttccc ctgtatgact tctcaaatga
     6241 agggtaaggg atgcaatttg agagaaggct ttaccacatt cattacaatc ataaggtttc
     6301 tctccagtat gaactttttg atgtgtaatg aagttttgct tttggctgaa ggctttacca
     6361 cattcattgc attcataggg tttctctcca gaatgaattt tttcatgagc aatgagattt
     6421 gatttctggc taaaggcttt cccacattcc ttacatatat agggtttttc cccagtatga
     6481 attctctggt gtctaacaag atttgacatc tgtataaaag ctttcccaca ttcattacac
     6541 tcataaggtt tctctctagt atgaattttc tggtgtgtaa tgaagttttt cttgtggctg
     6601 aaggcttttc tgcattcctt acattcatag ggtttctcac cagtgtgact tctcatatgt
     6661 acagtaaggg ctgagctttg agagaaggct tttccacact cattacattc atagggtttt
     6721 tcacctgtat gaattctaac atgtataata agcatggaaa actgagagaa ggctttacca
     6781 catttatcac atttataagg tttttctcct gtatgacttc tcatatgaag agcaagggat
     6841 gcaattcgag ggaaggcttt accacattca ttacatgcat aaggtttctc cccagtatga
     6901 actttctgat gtgcaatgag gctttgcttc tggctgaatg cttttccaca ctcattacat
     6961 tcataaggtt tctctccagt atgaattttt tcatgatcaa taagatttga tttctggctg
     7021 aaggacttct cacattcctt acatgcatag ggcttctctc cagtatgaat tctttgatgt
     7081 ctaatgagat ttgacatttt aatgaaagct ttaccacatt cattacattt gtaagactgc
     7141 tccctactgt gaattttata atgtttaata agtttttcct tgtgactgaa ggcttttcta
     7201 cagttactac attcataggg cttctctcca gtatgaattc tcagatgtct gatgaggtcc
     7261 aaagtctgat tgaagccttt tccacaatga ttacacttaa agggggtaat gacaaaatgg
     7321 gatgagctat taccatatga tttcacaggt tcattatatt cacaatgctc ctttctcatg
     7381 gtggcggtgc tagcctcacg acacctgaaa tggaagaaaa aaactttgaa ccactgtctg
     7441 aggcttgaga atgaaccaag atccaaactc aaaaagggca aattccaagg agaattacat
     7501 caagtgccaa gctggcctaa cttcagtctc cacccactca gtgtggggaa actccatcgc
     7561 ataaaacccc tccccccaac ctaaagacga cgtactccaa aagctcgaga actaatcgag
     7621 gtgcctggac ggcgcccggt actccgtgga gtcacatgaa gcgacggctg aggacggaaa
     7681 ggcccttttc ctttgtgtgg gtgactcacc cgcccgctct cccgagcgcc gcgtcctcca
     7741 ttttgagctc cctgcagcag ggccgggaag cggccatctt tccgctcacg caactggtgc
     7801 cgaccgggcc agccttgccg cccagggcgg ggcgatacac ggcggcgcga ggccaggcac
     7861 cagagcaggc cggccagctt gagactaccc ccgtccgatt ctcggtggcc gcgctcgcag
     7921 gccccgcctc gccgaacatg tgcgctggga cgcacgggcc ccgtcgccgc ccgcggcccc
     7981 aaaaaccgaa ataccagtgt gcagatcttg gcccgcattt acaagactat cttgccagaa
     8041 aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct agagacttat cgaaagcagc
     8101 gagacaggcg cgaaggtgcc accagattcg cacgcggcgg ccccagcgcc caggccaggc
     8161 ctcaactcaa gcacgaggcg aaggggctcc ttaagcgcaa ggcctcgaac tctcccaccc
     8221 acttccaacc cgaagctcgg gatcaagaat cacgtactgc agccaggtgg aagtaattca
     8281 aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac acacggcact
     8341 tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga ctactgcact
     8401 tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga catcactttc
     8461 ccagtttacc ccgcgccacc ttctctaggc accggttcaa ttgccgaccc ctccccccaa
     8521 cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcaccgg agccaattcc
     8581 cactcctttc aagacctaga aggtccatta gctgcaaaga ttcctctctg tttaaaactt
     8641 tatccatctt tgcacctgca ggcctcgagc cggcgccaaa gtggatctct gctgtccctg
     8701 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     8761 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     8821 cttttaaaat tgtggatgaa tactgccatt tgtctcaaga tctagaattc gttaactggc
     8881 accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt
     8941 gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa
     9001 ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag
     9061 acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt
     9121 gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct
     9181 caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg
     9241 cgtggtccag accgccacgg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag
     9301 cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg
     9361 atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc
     9421 cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggtaag
     9481 ctccggtggt acctctagaa agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     9541 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     9601 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     9661 gagttagggg cgggactatg gttgctgact aattgagacg catgctttgc atacttctgc
     9721 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcaatgcat
     9781 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     9841 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     9901 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaa
//
