LOCUS       sequence-466907-       11647 bp    DNA     circular SYN 10-JUN-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11647)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1385
FEATURES             Location/Qualifiers
     source          1..11647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(174..185)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             363..596
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    674..795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(711..730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     765..784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      835..970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(990..1009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(991..1009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1019..1036)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1019..1035)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1028..1050)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1177..1632
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1264..1283)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1474..1495
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1658..1762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1763..1831,1832..2623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1981..2000)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2794..3382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3283..3302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3536..3553
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3670..3691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3706..3711,3712..3729,3730..3736)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3744..3760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3749..3771
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3768..3784
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3768..3784
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3803..3823
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3805..3823
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3851..4077
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4078..4258
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4305..4430
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4923..5156
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5341..5385
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5616..5826
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5638..5834
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5685..5820
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5866..6468
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(5866..5885)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6362..6382
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6511..6628
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6766..7944
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6997..7935
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7892..7912
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(11350..11647,1..291)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11403..11423)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
       61 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
      121 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
      181 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
      241 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
      301 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
      361 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
      421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
      601 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
      661 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      721 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      781 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
      841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
      961 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     1021 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1081 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1141 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     1201 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     1261 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     1321 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     1381 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     1441 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     1501 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1561 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     1621 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1981 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2461 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2941 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3181 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3361 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3421 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3481 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3541 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3601 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     3661 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     3721 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     3781 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     3841 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     3901 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     3961 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     4021 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     4081 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4141 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4201 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     4261 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     4321 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     4381 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     4441 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     4501 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     4561 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     4621 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     4681 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     4741 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     4801 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     4861 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     4921 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     4981 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     5041 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     5101 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     5161 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     5221 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     5281 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     5341 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     5401 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     5461 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     5521 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt
     5581 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5641 gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5701 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5761 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5821 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag
     5881 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5941 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6001 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6061 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6121 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6181 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6241 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6301 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6361 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6421 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat
     6481 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     6541 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     6601 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     6661 ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat
     6721 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     6781 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     6841 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6901 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6961 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     7021 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     7081 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     7141 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     7201 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     7261 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     7321 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     7381 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     7441 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     7501 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     7561 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     7621 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     7681 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     7741 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     7801 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     7861 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     7921 tttcttccat ttcaggtgtc gtgaggctag caccgccacc atgagttggt tcaacgcctc
     7981 ccagctctcc agcttcgcta agcaggccct gtcccaggcc cagaagtcta ttgacagggt
     8041 tctggacatc caggaagagg agccgagcat ctgggccgag accattccgt atggagagcc
     8101 gggaataagt tcccctgtca gtggaggatg ggatacttca acctgggggt tgaaatcaaa
     8161 cactgaacct cagagtccac caatagcctc tcctaaagca atcacaaagc cagttcggag
     8221 gactgtggtc gatgaatctg aaaatttctt cagtgccttt ctctcgccaa ctgatgtcca
     8281 gaccattcag aagagtccag tggtatcaaa acctccagca aaatcacaac gaccagaaga
     8341 agaagtgaaa agcagcttac atgaatcctt gcacattggc cagtcaagaa ctcctgaaac
     8401 aactgaatca caagtaaaag actcttcttt gtgtgtttca ggggaaactc tggcagcagg
     8461 tacttcatca cctaaaactg aaggcaagca cgaagaaact gttaataaag aatcggatat
     8521 gaaggtgcca actgtaagtt tgaaagtatc tgaaagtgta attgatgtga aaacaactat
     8581 ggaaagtata tctaatacgt ctacgcagtc tctcacagca gaaacaaagg acatagcttt
     8641 ggaacctaag gaacaaaaac atgaagacag gcagagcaat acaccttctc ctcctgttag
     8701 taccttttca tcaggtactt ctaccaccag tgatattgaa gttttagatc atgaaagtgt
     8761 aataagtgag agctcagcga gctcgagaca agagactaca gattcaaaat caagtcttca
     8821 cttgatgcag acatcttttc agcttctctc tgcatctgct tgtcctgaat ataatcgttt
     8881 agatgatttc caaaaactca ctgagagttg ctgttcatct gatgcttttg aaagaataga
     8941 ctcatttagt gtacagtcat tagatagccg gagtgtaagt gaaatcaatt cagatgatga
     9001 attgtcaggc aagggatatg ctttagtgcc tattatagtt aattcttcaa ctccaaagtc
     9061 taaaacagtt gaatctgctg aaggaaaatc tgaagaagta aatgaaacat tagttatacc
     9121 cactgaggaa gcagaaatgg aagaaagtgg acgaagtgca actcctgtta actgtgaaca
     9181 gcctgatatc ttggtttctt ccacaccaat aaatgaagga cagactgtgt tagacaaggt
     9241 ggctgagcag tgtgaacctg ctgaaagtca gccagaagca ctttctgaga aggaagatgt
     9301 ttgcaagaca gttgaatttc tgaatgaaaa gctggaaaaa agggaggctc agttattatc
     9361 tcttagtaag gaaaaagcac ttctagaaga agcttttgat aacctgaaag atgaaatgtt
     9421 cagagtgaaa gaagaaagca gtagcatttc ttccttgaaa gatgagttta ctcaaagaat
     9481 tgcagaagca gaaaagaaag ttcaactagc ctgcaaagag agagatgctg ctaaaaagga
     9541 aatcaaaaac ataaaagaag aacttgccac tagattaaat agtagtgaaa ctgcagacct
     9601 tttgaaagag aaagatgagc agatccgagg gttaatggaa gaaggagaaa aactttcaaa
     9661 acagcagctg cacaattcta acatcatcaa gaaattaaga gctaaagaca aggagaatga
     9721 aaatatggtt gcaaagctga acaaaaaagt taaagagcta gaagaggagt tgcagcattt
     9781 gaaacaggtc cttgatggca aagaagaggt tgagaaacaa catagagaaa atattaaaaa
     9841 actaaattcc atggtagaac gccaagagaa agatcttggc cgtcttcagg tagacatgga
     9901 tgaacttgaa gaaaagaacc gaagtattca ggctgccctg gatagtgcat acaaagaact
     9961 tactgatctt cacaaagcca atgctgcaaa ggatagtgag gcacaggaag ctgctctgag
    10021 ccgtgaaatg aaagctaaag aagaactttc tgcagcatta gagaaggccc aagaagaagc
    10081 ccgtcagcag caagaaacat tagccattca agtgggggac cttaggcttg cattgcagcg
    10141 tacagaacaa gcggctgcca gaaaggagga ttatttacgc catgagatcg gtgaacttca
    10201 gcagagactc caggaagcag agaatcgaaa ccaggaactg agtcaaagtg tttcatcaac
    10261 aacaagacca ttgcttcgac aaatagaaaa tttgcaagca accctgggat cccagacatc
    10321 gtcgtgggag aaattagaga agaatctttc tgataggctt ggtgaatccc agaccttgct
    10381 ggcagcagca gttgagagag aacgtgcagc tacagaagaa ctccttgcta acaaaattca
    10441 gatgtcttcc atggagtcac agaattctct tttaagacag gaaaacagta gatttcaagc
    10501 ccagctagaa tcagagaaaa ataggctgtg taaactggag gatgagaaca ataggtacca
    10561 ggttgaattg gaaaacctaa aagatgaata tgtaagaaca cttgaagaga cgaggaaaga
    10621 aaagacattg ttgaatagtc agttagaaat ggaaagaatg aaagttgaac aagaaaggaa
    10681 gaaagccatt tttactcaag aaacaataaa agaaaaggaa cgcaagccat tttctgtttc
    10741 tagcactccc accatgtcac gctcaagttc aataagtggt gttgatatgg caggactaca
    10801 gacatctttt ctgtctcagg atgagtctca tgatcactca tttggaccaa tgcctatatc
    10861 agcaaatgga agcaatcttt atgatgctgt aaggatggga gcaggatcaa gcataattga
    10921 aaacctacag tctcagctaa agctaaggga aggggaaatc actcatttac agctagaaat
    10981 tggcaatcta gaaaaaactc gatcaataat ggctgaagaa ctagttaaat taacaaatca
    11041 aaatgatgaa cttgaagaga aggtgaagga gatacccaaa cttagaactc agctaagaga
    11101 tttggatcaa aggtacaaca ctattctgca gatgtatgga gaaaaagcag aagaggcaga
    11161 agaacttcga ttagatctcg aagatgtaaa aaatatgtac aaaactcaaa tagatgaact
    11221 tttaagacaa agtctcagtt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
    11281 ttggctttat atatcttgtg gaaaggacga aacctgactt cgtacgatcc tgttacgcgt
    11341 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    11401 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    11461 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    11521 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    11581 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    11641 cccctcc
//
