LOCUS       sequence-466912-        5085 bp    DNA     circular SYN 15-APR-2025
DEFINITION  DsREDmonomer-based BiFC vector for plants (Positive control: DXMT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBRN168-DXMT
FEATURES             Location/Qualifiers
     source          1..5085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..367
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     310..330
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    387..442
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     860..879
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2141..2393
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2431..2447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2431..2447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2444..2466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2455..2471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2479..2485,2486..2503,2504..2509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2524..2545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2662..2679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2833..3421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2913..2932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3592..4383,4384..4452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4215..4234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4453..4557)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4625..4643
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4681..4703)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4803..4822
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5016..5038
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5030..5047
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5031..5047
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
       61 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
      121 ctcctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      181 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      241 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      301 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      361 gaggacaggc ttcttgagat ccttcaacaa ttaccaacaa caacaaacaa caaacaacat
      421 tacaattact atttacaatt acagtcgact ctagaggatc catggacaac accgaggacg
      481 tcatcaagga gttcatgcag ttcaaggtgc gcatggaggg ctccgtgaac ggccactact
      541 tcgagatcga gggcgagggc gagggcaagc cctacgaggg cacccagacc gccaagctgc
      601 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt
      661 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacatg aagctgtcct
      721 tccccgaggg cttcacctgg gagcgctcca tgaacttcga ggacggcggc gtggtggagg
      781 tgcagcagga ctcctccctg caggacggca ccttcatcta caaggtgaag ttcaagggcg
      841 tgaacttccc cgccgacggc cccgtaatgc agaagaagac tgccggctgg gagccctcca
      901 ccgagaagct gtacccccag gacggcgtgc tgaagggcga gatctcccac gccctgaagc
      961 tgaaggacat ggagctccaa gaagtcctgc atatgaatgg aggcgaaggc gatacaagct
     1021 acgccaagaa ctcattctac aatctgtttc tcatcagggt gaaacctatc cttgaacaat
     1081 gcatacaaga attgttgcgg gccaacttgc ccaacatcaa caagtgcatt aaagttgcgg
     1141 atttgggatg cgcttctgga ccaaacacac ttttaacagt tcgggacatt gtacaaagta
     1201 ttgacaaagt tggccaggaa aagaagaatg aattagaacg tcccaccatt cagatttttc
     1261 tgaatgatct tttccaaaat gatttcaatt cggttttcaa gtcgctgcca agcttctacc
     1321 gcaaacttga gaaagaaaat ggacgcaaaa taggatcatg cctgataggc gcaatgcctg
     1381 gctctttcta cggcagactc ttccccgagg agtccatgca ttttttacac tcttgttact
     1441 gtttgcattg gttatctcag gttcccagcg gtttggtgac tgaattgggg atcagtgcga
     1501 acaaagggtg catttactct tccaaagcaa gtcgtccgcc catccagaag gcatatttgg
     1561 atcaatttac gaaagatttt accacatttc ttaggattca ttcggaagag ttgatttcac
     1621 gtggccgaat gctccttact tggatttgca aagaagatga attcgagaac ccgaattcca
     1681 tagacttact tgagatgtca ataaacgact tggttattga gggacatctg gaggaagaaa
     1741 aattggacag tttcaatgtt ccaatctatg caccttcaac agaagaagta aagtgcatag
     1801 ttgaggagga aggttctttt gaaattttat acctggagac ttttaaggtc ccttatgatg
     1861 ctggcttctc tattgatgat gattaccaag gaagatccca ttccccagta tcctgcgatg
     1921 aacatgctag agcagcgcat gtggcatctg tcgttagatc aattttcgaa cccatcgtcg
     1981 caagtcattt tggagaagct atcatgcctg acttatccca caggattgcg aagaatgcag
     2041 caaaggttct tcgctccggc aaaggcttct atgatagtct tatcatttct ctcgccaaaa
     2101 agccggagaa ggcagacatg taagcggccg cccggctgca gatcgttcaa acatttggca
     2161 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     2221 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     2281 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     2341 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atccgatgat
     2401 aagctgtcaa acatgagaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     2461 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2521 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2581 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2641 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3241 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3601 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3661 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3721 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3781 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3841 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3901 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3961 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4021 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4081 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4141 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4561 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4621 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4681 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4741 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     4801 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     4861 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     4921 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     4981 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     5041 ggccagtgcc aagcttgcat gcctgcaggc tctagaggat ccccc
//
