LOCUS       sequence-466946-        4210 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A gateway compatible 5' entry clone containing the murine Dll4 F2 
            arterial specific enhancer upstream of a minimal E1b promoter and 
            intron for arterial and endocardial expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Gillespie W, Zhang Y, Ruiz OE, Cerda III J, Ortiz-Guzman J, Turner 
            WD, Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien C-B, Kwan KM,
            Arenkiel BR, Devine WP, Wythe JD
  TITLE     Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox with diverse applications in vertebrate model 
            systems
  JOURNAL   bioRxiv 2024.07.13.603267
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.07.13.603267"
COMMENT     Sequence Label: p5E-Dll4-F2-E1B-B-Globin (JDW 1366)
FEATURES             Location/Qualifiers
     source          1..4210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(919..946)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1038..1124)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1173..1195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1187..1204
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1188..1204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1244..1339
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          2235..2807
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     protein_bind    2907..3030
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3102..3121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3103..3121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3126..3142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3126..3142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3244..3263)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3255..4064
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3322..3341)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      901 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      961 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     1021 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1081 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     1141 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     1201 cagtcttaag ctcgggcccg agttaacgct accatggagc tccaaataat gattttattt
     1261 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgcttttt tataatgcca
     1321 actttgtata gaaaagttga agcttctcga gagtatctaa cttctcggcc acaggggggc
     1381 gacatcacac agcgccgaaa gagttaacca gttataggcg ggggtggggg ttggggacgc
     1441 aggcttgggg gtgggggcca ggacgcttag cttggccgga gctgcgcccc gcgctggacg
     1501 ctcggattcc gctcgctgcc tggactcaga gcacaattgc gtttcctgcg ggttattttt
     1561 ggcgtgggaa cgcggggagc gcggcggtga gaaaggccga ggctgccagc gccgctgacg
     1621 ggcctcttcc tgtattttac accttttgcg aattccgctc ctttggaaag ggaataatgg
     1681 ctttgggatg ttgttctgac acagaggaaa aggatatttc accagcacaa caattctcac
     1741 tttgaaaagg aaaaagaaaa accattacct acgtctagaa cagaacccct tgctcccagt
     1801 tctcgaacca gaaaacttcc ccctttaaat tttttctttt tttccatttt gacctctttt
     1861 cctctttccc ctccgtatct gcctccacaa ccctaggata tcttaacatc cgtccattgt
     1921 accctttttt gaatgctatc aagccccctg cacatgcaca cacccaggga gactaagtag
     1981 caagattctg ggaccctctg gcctgtgctt acttgcaggt agagttaatc tagataatta
     2041 gagtgtgaac tgaccaccat agtcacaact aaagagagag ttggcagcag tcaactctct
     2101 ctgaatcagg ttggctttct gaatcaggtt ctctgaccaa agcctctttc tgcgcggccg
     2161 cactagttcg actctagagg gtatataatg gatcccatcg cgtctcagca gatctcgatc
     2221 ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa
     2281 aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatttc
     2341 ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac
     2401 aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct
     2461 tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc
     2521 tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt
     2581 agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct
     2641 ggcgtggaaa tattcttatt ggtagaaaca actacaccct ggtcatcatc ctgcctttct
     2701 ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac
     2761 cgggcccctc tgctaaccat gttcatgcct tcttctcttt cctacagctc ctgggcaacg
     2821 tgctggttgt tgtgctgtct catcattttg gcaaagaatt cctcgacgtc gacaccggta
     2881 tcgatacgcg ttacgtagcg gccgctcaag tttgtacaaa aaagttgaac gagaaacgta
     2941 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     3001 ctgtaaaaca caacatatgc agtcactatg aaccaactac ttagatggta ttagtgacct
     3061 gtagaattcg agctctagag ctgcagggcg gccgcgatat cccctatagt gagtcgtatt
     3121 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     3181 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     3241 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     3301 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     3361 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     3421 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     3481 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     3541 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     3601 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     3661 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     3721 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     3781 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     3841 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     3901 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     3961 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     4021 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     4081 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     4141 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     4201 aaggatcttc
//
