LOCUS       sequence-467128-       10186 bp    DNA     circular SYN 15-APR-2025
DEFINITION  SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication 
            origin and split-Nat selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  TITLE     Sequencing of a dairy isolate unlocks Kluyveromyces marxianus as a 
            host for lactose valorization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMTKM02
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(129..717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(209..228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             1556..1567
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1574..2287
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2299..2370
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2386..2413
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        2427..2502
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2636..2978
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3877..4074
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(join(4225..5016,5017..5085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4848..4867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5086..5190)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5893..9996
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5893..9996
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10006..10026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10042..10065
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      join(10069..10186,1..72)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
       61 ggacgctcga agtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      721 gatcctttga tcttttctac actagttcaa catctttgga taatatcaga atgagaaaga
      781 acagatacgc agtacgtttt ttggtgagct ctttgcactt ctttagttct ttccatcaat
      841 atcagttttt taaactttta ggactaaaag tgatgtttaa cttcaaaatg tttaaaattt
      901 tgttcttccc gacgttcatt aagaatacta atacacttta ataattattt taatattttg
      961 ttctaaataa tgacttttaa ttaaaaaaga taaaatataa aaacatcata ataactcacc
     1021 agaggttaag aacaaaaaaa caaattagat atctgctaat ccaatatagt taaatcaatc
     1081 tttccttggt ataatgggta tattacatat atttcaagga ccgacactcc taccaaatat
     1141 ctaaaattta ccatattaac ataacatgta tataaacgtc aaatcataat cagcactagt
     1201 atatgtgtta tgtagtatac tctttcttca acaattaaat actctcggta gccaagttgg
     1261 tttaaggcgc aagactgtaa tttatcacta cgaaatcttg agatcgggcg ttcgactcgc
     1321 ccccgggaga gatggccggc atggtcccag cctcctcgct ggcgccggct gggcaacacc
     1381 ttcgggtggc gaatgggact cgagaccgaa agtgaaacgt gatttcatgc gtcattttga
     1441 acattttgta aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt
     1501 ttcttaacat cgcggcaact caagaaacgg caggttcgga tcttagctac tagagaaaga
     1561 ggagaaatac tagatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt
     1621 ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga
     1681 cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggttcc
     1741 ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga
     1801 ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg
     1861 cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg
     1921 cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagagg acggcaatat
     1981 cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca ccgccgataa
     2041 acaaaaaaat ggcattaaag cgaattttaa aattcgccac aacgtggagg atggcagcgt
     2101 gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc
     2161 agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga
     2221 tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact
     2281 gtacaaatga ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2341 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2401 gggcctttct gcgtttatag gtctccgttt tagagctaga aatagcaagt taaaataagg
     2461 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttta ttttttgtca
     2521 ctattgttat gtaaaatgcc acctctgaca gtatggaacg caaacttctg tctagtggat
     2581 ataggtctag agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     2641 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     2701 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     2761 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     2821 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     2881 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     2941 aggatacagt tctcacatca catccgaaca taaacaacaa tgggtactac cttagatgat
     3001 acagcctaca gatacagaac atcagtccct ggtgatgctg aagcaattga ggctttagac
     3061 ggttcattca ccaccgacac cgtctttaga gtaaccgcca ccggtgatgg atttacctta
     3121 agagaagtcc cagtcgaccc tccattaact aaagtctttc cagatgatga atctgatgac
     3181 gaaagcgacg acggagaaga tggtgaccca gattcaagaa ctttcgtagc atacggtgat
     3241 gacggtgatt tggctggttt tgtagtcgtt tcttattcag gttggaatag aaggttgacc
     3301 gttgaagata tagaagtcgc cccagagcat agaggtcatg gtgtaggaag agctttgatg
     3361 ggtttggcta cagaatttgc aagagagaga ggagccggtc atttatggtt gacgtcgctg
     3421 tcctgcagga tttaccttaa gagaagtccc agtcgaccct ccattaacta aagtctttcc
     3481 agatgatgaa tctgatgacg aaagcgacga cggagaagat ggtgacccag attcaagaac
     3541 tttcgtagca tacggtgatg acggtgattt ggctggtttt gtagtcgttt cttattcagg
     3601 ttggaataga aggttgaccg ttgaagatat agaagtcgcc ccagagcata gaggtcatgg
     3661 tgtaggaaga gctttgatgg gtttggctac agaatttgca agagagagag gagccggtca
     3721 tttatggtta gaagttacta atgttaacgc ccctgctatc catgcttata gaagaatggg
     3781 tttcacatta tgtggtttag atactgcttt atatgatgga acagcatctg acggtgaaca
     3841 ggccttgtat atgtctatgc cttgccctta atcagtactg acaataaaaa gattcttgtt
     3901 ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat
     3961 gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt
     4021 gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg
     4081 attcgatact aacgccgcca tccagtgtcg aaaacgagct ctcgagaacc cttaatcaaa
     4141 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4201 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4261 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4321 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
     4381 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4441 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4501 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4561 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4621 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4681 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4741 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4801 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4861 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4921 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4981 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5041 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5101 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5161 ttagaaaaat aaacaaatag gggttcgtcg acagtcgaac aagaagcagg caaagtttag
     5221 agcactgccc ctccgcactc aaaaaagaaa aaactaggag gaaaataaaa ttctcaacca
     5281 cacaaacaca taaacacata caaatacaaa tacaagctta tttacttgac atcgcgcgat
     5341 cttccactat tcagcgccgt ccgccctctc tcgtgttttt tgtttacgcg acaactatgc
     5401 gaaatccgga gcaacgggca accgtttggg gaaagaccac acccacgcgc gatcgccatg
     5461 gcaacgaggt cgcacacgcc ccacacccag acctccctgc gagcgggcat gggtacaatg
     5521 tccccgttgc cacagacacc acttcgtagc acagcgcaga gcgtagcgtg ttgttgctgc
     5581 tgacaaaaga aaatttttct tagcaaagca aaggagggga agcacgggca gatagcaccg
     5641 taccataccc ttggaaactc gaaatgaacg aagcaggaaa tgagagaatg agagttttgt
     5701 aggtatatat agcggtagtg tttgcgcgtt accatcatct tctggatcta tctattgttc
     5761 ttttcctcat cactttcccc tttttcgctc ttcttcttgt cttttatttc tttctttttt
     5821 ttaattgttc cctcgattgg ctatctacca aagaatccaa acttaataca cgtatttatt
     5881 tgtccaatta ccatggataa gaaatactca ataggcttag atatcggcac aaatagcgtc
     5941 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
     6001 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
     6061 gagacagcgg aagcgactcg actcaaacgg acagctcgta gaaggtatac acgtcggaag
     6121 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
     6181 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
     6241 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
     6301 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
     6361 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
     6421 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
     6481 gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg
     6541 agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc
     6601 ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt
     6661 gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat
     6721 aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta
     6781 tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc
     6841 ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa
     6901 gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa
     6961 aacggatatg caggttatat tgatggggga gctagccaag aagaatttta taaatttatc
     7021 aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa
     7081 gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg
     7141 ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat
     7201 cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg
     7261 cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg
     7321 aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca
     7381 aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag
     7441 tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa
     7501 ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat
     7561 cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat
     7621 agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat
     7681 ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta
     7741 gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt
     7801 aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat
     7861 actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc
     7921 aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg
     7981 atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa
     8041 ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt
     8101 attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca
     8161 gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat
     8221 tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt
     8281 aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc
     8341 caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat
     8401 gatgtcgatc acattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc
     8461 ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc
     8521 aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag
     8581 tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt
     8641 atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat
     8701 agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt
     8761 accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt
     8821 gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct
     8881 ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat
     8941 gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat
     9001 ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag
     9061 attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa
     9121 gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag
     9181 aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg
     9241 gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt
     9301 ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag
     9361 ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa
     9421 aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt
     9481 aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt
     9541 gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta
     9601 tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa
     9661 ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt
     9721 tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa
     9781 catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg
     9841 aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat
     9901 acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat
     9961 gaaacacgca ttgatttgag tcagctagga ggtgacggtg gagggccaaa aaagaaaaga
    10021 aaagttgaag atgcttctgc tcatcatcac catcaccatc accattaaat ccgctctaac
    10081 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat
    10141 gttagtatta agaacgttat ttatatttca aatttttctt tttttt
//
