LOCUS       sequence-467129-       10622 bp    DNA     circular SYN 15-APR-2025
DEFINITION  SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication 
            origin and split-G418 selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10622)
  TITLE     Sequencing of a dairy isolate unlocks Kluyveromyces marxianus as a 
            host for lactose valorization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMTKM04
FEATURES             Location/Qualifiers
     source          1..10622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(129..717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(209..228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             1556..1567
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1574..2287
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2299..2370
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2386..2413
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        2427..2502
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2636..2978
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(3047..3066)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      4313..4510
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(join(4661..5452,5453..5521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5284..5303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5522..5626)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6329..10432
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6329..10432
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10442..10462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10478..10501
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      join(10505..10622,1..72)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
       61 ggacgctcga agtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      721 gatcctttga tcttttctac actagttcaa catctttgga taatatcaga atgagaaaga
      781 acagatacgc agtacgtttt ttggtgagct ctttgcactt ctttagttct ttccatcaat
      841 atcagttttt taaactttta ggactaaaag tgatgtttaa cttcaaaatg tttaaaattt
      901 tgttcttccc gacgttcatt aagaatacta atacacttta ataattattt taatattttg
      961 ttctaaataa tgacttttaa ttaaaaaaga taaaatataa aaacatcata ataactcacc
     1021 agaggttaag aacaaaaaaa caaattagat atctgctaat ccaatatagt taaatcaatc
     1081 tttccttggt ataatgggta tattacatat atttcaagga ccgacactcc taccaaatat
     1141 ctaaaattta ccatattaac ataacatgta tataaacgtc aaatcataat cagcactagt
     1201 atatgtgtta tgtagtatac tctttcttca acaattaaat actctcggta gccaagttgg
     1261 tttaaggcgc aagactgtaa tttatcacta cgaaatcttg agatcgggcg ttcgactcgc
     1321 ccccgggaga gatggccggc atggtcccag cctcctcgct ggcgccggct gggcaacacc
     1381 ttcgggtggc gaatgggact cgagaccgaa agtgaaacgt gatttcatgc gtcattttga
     1441 acattttgta aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt
     1501 ttcttaacat cgcggcaact caagaaacgg caggttcgga tcttagctac tagagaaaga
     1561 ggagaaatac tagatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt
     1621 ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga
     1681 cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggttcc
     1741 ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga
     1801 ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg
     1861 cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg
     1921 cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagagg acggcaatat
     1981 cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca ccgccgataa
     2041 acaaaaaaat ggcattaaag cgaattttaa aattcgccac aacgtggagg atggcagcgt
     2101 gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc
     2161 agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga
     2221 tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact
     2281 gtacaaatga ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2341 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2401 gggcctttct gcgtttatag gtctccgttt tagagctaga aatagcaagt taaaataagg
     2461 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttta ttttttgtca
     2521 ctattgttat gtaaaatgcc acctctgaca gtatggaacg caaacttctg tctagtggat
     2581 ataggtctag agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     2641 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     2701 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     2761 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     2821 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     2881 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     2941 aggatacagt tctcacatca catccgaaca taaacaacaa tgggtaagga aaagactcac
     3001 gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3061 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3121 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3181 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3241 actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc attccaggta
     3301 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3361 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctg
     3421 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3481 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     3541 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3601 aaattaatag gttgtattga tgttggacga gtcggaatcg acgtcgctgc ctgcaggatg
     3661 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3721 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3781 tcaccactgc gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag
     3841 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     3901 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctggc tcaggcgcaa tcacgaatga
     3961 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     4021 aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg
     4081 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     4141 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     4201 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     4261 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gtactgacaa
     4321 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt
     4381 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc
     4441 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt
     4501 cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctctcg
     4561 agaaccctta atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4621 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4681 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4741 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4801 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4861 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4921 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4981 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5041 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5101 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5161 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5221 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5281 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5341 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5401 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5461 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5521 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5581 acatatttga atgtatttag aaaaataaac aaataggggt tcgtcgacag tcgaacaaga
     5641 agcaggcaaa gtttagagca ctgcccctcc gcactcaaaa aagaaaaaac taggaggaaa
     5701 ataaaattct caaccacaca aacacataaa cacatacaaa tacaaataca agcttattta
     5761 cttgacatcg cgcgatcttc cactattcag cgccgtccgc cctctctcgt gttttttgtt
     5821 tacgcgacaa ctatgcgaaa tccggagcaa cgggcaaccg tttggggaaa gaccacaccc
     5881 acgcgcgatc gccatggcaa cgaggtcgca cacgccccac acccagacct ccctgcgagc
     5941 gggcatgggt acaatgtccc cgttgccaca gacaccactt cgtagcacag cgcagagcgt
     6001 agcgtgttgt tgctgctgac aaaagaaaat ttttcttagc aaagcaaagg aggggaagca
     6061 cgggcagata gcaccgtacc atacccttgg aaactcgaaa tgaacgaagc aggaaatgag
     6121 agaatgagag ttttgtaggt atatatagcg gtagtgtttg cgcgttacca tcatcttctg
     6181 gatctatcta ttgttctttt cctcatcact ttcccctttt tcgctcttct tcttgtcttt
     6241 tatttctttc ttttttttaa ttgttccctc gattggctat ctaccaaaga atccaaactt
     6301 aatacacgta tttatttgtc caattaccat ggataagaaa tactcaatag gcttagatat
     6361 cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa
     6421 gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct
     6481 tttatttgac agtggagaga cagcggaagc gactcgactc aaacggacag ctcgtagaag
     6541 gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc
     6601 gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa
     6661 gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa
     6721 atatccaact atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt
     6781 gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga
     6841 gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac
     6901 ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat
     6961 tctttctgca cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg
     7021 tgagaagaaa aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa
     7081 ttttaaatca aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta
     7141 cgatgatgat ttagataatt tattggcgca aattggagat caatatgctg atttgttttt
     7201 ggcagctaag aatttatcag atgctatttt actttcagat atcctaagag taaatactga
     7261 aataactaag gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga
     7321 cttgactctt ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt
     7381 ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga
     7441 attttataaa tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt
     7501 gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc
     7561 ccatcaaatt cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc
     7621 atttttaaaa gacaatcgtg agaagattga aaaaatcttg acttttcgaa ttccttatta
     7681 tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga
     7741 aacaattacc ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt
     7801 tattgaacgc atgacaaact ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca
     7861 tagtttgctt tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac
     7921 tgaaggaatg cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt
     7981 actcttcaaa acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa
     8041 aatagaatgt tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt
     8101 aggtacctac catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga
     8161 aaatgaagat atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat
     8221 gattgaggaa agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct
     8281 taaacgtcgc cgttatactg gttggggacg tttgtctcga aaattgatta atggtattag
     8341 ggataagcaa tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg
     8401 caattttatg cagctgatcc atgatgatag tttgacattt aaagaagaca ttcaaaaagc
     8461 acaagtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc
     8521 tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat
     8581 ggggcggcat aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca
     8641 aaagggccag aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt
     8701 aggaagtcag attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct
     8761 ctatctctat tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa
     8821 tcgtttaagt gattatgatg tcgatcacat tgttccacaa agtttcctta aagacgattc
     8881 aatagacaat aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc
     8941 aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt
     9001 aatcactcaa cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact
     9061 tgataaagct ggttttatca aacgccaatt ggttgaaact cgccaaatca ctaagcatgt
     9121 ggcacaaatt ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg
     9181 agaggttaaa gtgattacct taaaatctaa attagtttct gacttccgaa aagatttcca
     9241 attctataaa gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc
     9301 cgtcgttgga actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg
     9361 tgattataaa gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa
     9421 agcaaccgca aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac
     9481 acttgcaaat ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga
     9541 aattgtctgg gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca
     9601 agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg agtcaatttt
     9661 accaaaaaga aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata
     9721 tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa
     9781 agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa ttatggaaag
     9841 aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa
     9901 aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa
     9961 acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa
    10021 atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga
    10081 taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga
    10141 gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct
    10201 tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca
    10261 tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat
    10321 tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc
    10381 catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg acggtggagg
    10441 gccaaaaaag aaaagaaaag ttgaagatgc ttctgctcat catcaccatc accatcacca
    10501 ttaaatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
    10561 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
    10621 tt
//
