LOCUS       sequence-467222-        2361 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the PST1 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2361)
  TITLE     Sequencing of a dairy isolate unlocks Kluyveromyces marxianus as a 
            host for lactose valorization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMTKM09
FEATURES             Location/Qualifiers
     source          1..2361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(726..760)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(831..1490)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1405..1424
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1672..2260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2161..2180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcatttca gagttctttt gtctttttta atcttcaatc ttcggctttt
       61 tgtcatctta ttaacataca attcaagtac atttacattt acaatcacaa tcacaatcaa
      121 tcatcagtca ttggatcttt ttctcttccg aaaagattcg tttaaacaaa ttaagataat
      181 gcatataaca aagtacatcg ttaaagaaca tttaacagta tagttcatca tatagtaacc
      241 acaacattac atggatattc tgtcaaaaac agagtagttg gtttcaaccc tacttaattg
      301 tttttttttt tttttttttt ttttgatata gctgaagagt aaaaaagtct tgacaatgaa
      361 aaaacaaaaa tccagaaggg aaaaatagtg acaggcgaca aatgacagat gacagaaagc
      421 agagattttc ggttgaaaac aaaaacagta tggaacagtg actgacgcaa aaccacaaag
      481 atccaaaacg tgccaaaata cccttttgtg ttgagcaatt ttcaaatata taaatatgcg
      541 acacttgatg aatacaatag aagcttccta tacattatta taattatctt tgttattgag
      601 acagagtatt tttgatattg ttttcagttc agaatactgt aagtacaggg cttctaatat
      661 tctaggggag tatattataa gtggagtgac ctgttcatat taacagatta tgtgagacca
      721 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      781 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      841 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      901 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      961 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1021 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     1081 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1141 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1201 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1261 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1321 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1381 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1441 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1501 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1561 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1801 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1921 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2041 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2161 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2221 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2281 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2341 tgattctgtg gataaccgta g
//
