LOCUS       sequence-467255-        2370 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the HSP26 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2370)
  TITLE     Sequencing of a dairy isolate unlocks Kluyveromyces marxianus as a 
            host for lactose valorization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMTKM17
FEATURES             Location/Qualifiers
     source          1..2370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(735..769)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(840..1499)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1414..1433
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1681..2269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2170..2189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcgcgcat gacaagggtg catgtcccct tggcacaggg ttgactcttt
       61 ttgagacaca acacctgtgt gcctggcttg gcctggccta gcctggcctg gcctagcacc
      121 atggggccgc ttcttagggg agccataatt ctcgggcata gaaaaccccg tagtgacggt
      181 tgctagaaat ttctggactg gcatcgaaaa agtattaccc ggctgaatta tagaggaaag
      241 ttagcgttat tttcattgat tcgcttttct ttttgtagtt gccgacagtg cggaacagcg
      301 acagtttaat gaatgtgatg agctaaggca aagggtaatt agaattagac actagaacga
      361 aggggaaata gggaggaaga tgttacgaga gagttctttg catgtagttg taaggtgatt
      421 agggattgtg taataacttt aatccatata gtagaaagtt ctaatggttt ctaaggggta
      481 gttgctgtgg tatacagtgc gccagtatta ttggtttggc aacagtgatg gatttggtgg
      541 acctgaaacg tataaattag acacgagtct ggcaagttga tgagagatgg gaaggttgtt
      601 tcttgtatta attgtattca gtgttatttc tatagcccca atcaccagag atatttgatt
      661 tcataagaga ctatatctaa gagattaaca acaactaaga acaaaagcga ttacgagtat
      721 gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      781 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      841 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      901 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      961 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1021 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
     1081 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1141 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1201 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1261 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1321 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1381 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1441 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1501 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1561 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     1621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2341 gttatcccct gattctgtgg ataaccgtag
//
