LOCUS       sequence-467294-        8954 bp    DNA     circular SYN 15-APR-2025
DEFINITION  PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry 
            as marker, EGFP as output) for stable integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ054 (PB)SFFV-LIDARsensor (hygR)
FEATURES             Location/Qualifiers
     source          1..8954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2479..2886
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2894..2903
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2900..3607
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3045..3063)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3313..3332
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3653..3718
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3887..3952
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(3959..3961,3962..4672)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             4694..4723
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4779..4796)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4785..4992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5025..5045)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5030..5359
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5210..5345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5272..5291
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5408..6433
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5408..6433
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(6493..6513)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6555..6676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6592..6611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6646..6665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7439..7473
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7964..7980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7964..7980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7977..7999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7988..8004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8012..8018,8019..8036,8037..8042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8057..8078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8195..8212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8366..8954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8446..8465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catcccagag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccaca attgccgata
     2401 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     2461 tttggcaagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga
     2521 atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga
     2581 tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt
     2641 tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt
     2701 ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct
     2761 tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag
     2821 ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg
     2881 cccgggaagc ttcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     2941 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     3001 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     3061 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     3121 caaggcctac gtgaagcacc ccgccgacat tcccgactac ttgaagctgt ccttccccga
     3181 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     3241 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     3301 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     3361 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     3421 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     3481 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     3541 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     3601 gtacaagagc gacgcgttgg aagccagaca caaacagaaa attgtggctc cggtgaaaca
     3661 gactttgaat tttgaccttc tgaagctggc gggagacgtc gagtccaacc ccggtcccgg
     3721 taccggatcc tccgtttagg tgggtggaat agtaacgtac accatcaggg tacgtctcag
     3781 acaccatcag ggtctgtctg gtacagcatc agcgtaccat agtatcccac ctacccagac
     3841 gaccggtact agtgaggcac ggcataagca aaagatcgta gccccagtaa agcaaacact
     3901 caacttcgat ctactcaaac tcgcaggtga tgtggaatct aatccaggac cctcgagtgt
     3961 ctcaaagggc gaggaacttt ttacgggcgt tgttccgatc ctcgtcgaat tggacgggga
     4021 cgttaatggg cataaattct cagttagtgg tgagggtgag ggggacgcga catatgggaa
     4081 acttacgctg aagttcatat gtaccaccgg aaaattgcca gtgccgtggc ctactctcgt
     4141 caccaccctg acatatggtg ttcagtgttt tagccggtat ccagaccata tgaaacagca
     4201 cgattttttt aaatccgcta tgcctgaagg ttacgtacag gagcgaacga tattttttaa
     4261 agacgacgga aactacaaaa ctagagccga ggtaaaattc gagggtgaca ctctcgtgaa
     4321 ccggatcgaa ctgaagggca tagattttaa ggaggatggc aatatactgg gccacaagct
     4381 cgaatacaac tataattctc ataatgtcta tatcatggcg gacaaacaga aaaacggcat
     4441 caaggtaaac tttaaaatcc gacacaatat tgaggacggc tctgtccagc ttgcggacca
     4501 ttaccaacag aacacgccga taggggatgg tccagtactt ctccctgata atcactattt
     4561 gtctactcag tcagctctta gcaaggaccc aaacgaaaaa cgggatcaca tggtccttct
     4621 tgagttcgtc actgcggctg ggataacact cgggatggac gaactgtata agtaggccgc
     4681 tcgaggaggg cccgaacaaa aactcatctc agaagaggat ctgaatagcg ccgtcgacca
     4741 tcatcataat catcattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     4801 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4861 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4921 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4981 gcatgctggg gacgcggccg caatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5041 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5101 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5161 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5221 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5281 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     5341 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     5401 gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     5461 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     5521 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     5581 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     5641 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     5701 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     5761 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     5821 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     5881 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     5941 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     6001 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     6061 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     6121 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     6181 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     6241 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     6301 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     6361 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     6421 ggcaaaggaa taggtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6541 tcatgcgtta actaaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6661 tcatcaatgt atcttatcat gtctggaatt gactcaaatg atgtcaatta gtctatcaga
     6721 agctcatctg gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt
     6781 gttcccaaac tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt
     6841 cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac
     6901 tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg
     6961 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     7021 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     7081 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     7141 gggctttggg ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt
     7201 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     7261 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     7321 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     7381 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     7441 tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc
     7501 gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag
     7561 gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat
     7621 gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt
     7681 gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg
     7741 tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac
     7801 aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg
     7861 ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg
     7921 ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg
     7981 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8041 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8101 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8161 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8221 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8281 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8341 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8401 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8461 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8521 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8581 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8641 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8701 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8761 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8821 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8881 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8941 aaaaaggatc tcaa
//
