LOCUS       sequence-467486-        9195 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses LbNOX in mouse cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  TITLE     Genetically encoded tool for manipulation of Delta-Psi-m identifies 
            the latter as the driver of integrative stress response induced by 
            ATP synthase dysfunction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-TRE3G-LbNOX-Flag
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1119..1752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1959..2547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2065..2076
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2474..2494
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2564..3163)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2647..2667)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3144..3163
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3184..3683)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3232..3251)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3625..3648
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(3735..3758)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5156..5505)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(5193..5217)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5263..5281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5299..5317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5335..5353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5371..5389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5407..5425
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5443..5461
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5479..5497
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    5568..5683
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(5946..5990)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6175..6408
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6905..7030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7077..7710
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7878..8012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7902..7921)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7956..7975
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8060..8164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8165..8233,8234..9025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8383..8402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgc ccgtcaggca ccgggcttgc
     2581 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2641 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     2701 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     2761 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     2821 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     2881 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     2941 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     3001 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt
     3061 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     3121 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca
     3181 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     3241 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     3301 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     3361 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     3421 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     3481 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     3541 accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag
     3601 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     3661 tgggaaaagc gcctccccta cccggtagaa ttccatatga cgcgtcccgg ggcggccgcg
     3721 ggcccggatc cttacttgtc atcgtcatcc ttgtaatcca tagatccacc agatccacca
     3781 gatccaccag cgttgacgga ttgggccact ttagcttggg cagcctgggc gagaatgttc
     3841 aggtagttga agggcctgtc gaaattgggc tggaacagca tgtccaccat ggccaggtca
     3901 tcgatggtgt tctcgttctg gatgcagacg gacagggtat tagcgctctg ggacacgtcg
     3961 tatttggaca tcagggcgcc tcccaggatt ctgtgtgtat cggggtcgaa gaccaggctc
     4021 atgaggacgg gctcggtgga aggcatgaac tcgggcctat agttgtcctc cacgatgacc
     4081 tgctcggcat tgaggccttg ttgcttggcg gcggccagtg tgaggcctgt ggagacaatt
     4141 gtcctgtcat agagggccag tccggagctg gactgggttc ccatgtactt cactgtgggc
     4201 ttgacgaggt tctttccgac gaggattccc tgcctgacgg cgttggtagc gaggggaatg
     4261 taggcgtttt ggtgtgtggg gttgtaatgg acggcggcgg agtcgccagc agcgaaaata
     4321 tcagggttgc tggacctcat gtagtcgtcg gtaatgatgg cgccgttagg ggccatgtcc
     4381 actttgcctt tcagcaggtc tgtgttgggc ctaaagccaa tgcagaggat ggccaggtcg
     4441 gtctcatagg agttcttgtc tgtcttgatt gtgaggccgg tggcgctgtc ggtgaagctc
     4501 tccactgtct cgcccagagc gagttgcacg ccatggtccc tgtagtcctg ttcgatgacg
     4561 tcggtgaagt cggcatcgaa gtacttgggc atgaccctag ccatggcgtc aatcagggtg
     4621 acgtcgtggc ctgttgtgga gtaggcctcg gccagttcgg ctccgatgta tccggcgccg
     4681 atgacggtga tcctcttggc ctccttggcg tcctcaatca gagcctgggc gtgggcccaa
     4741 ttcttgcaga gcttcaccct atcgctgtcg atgccgggga ttttgggcac gataggccag
     4801 cttccggagg tcatcaccag tttgtcgtag gactcggtgg tctgggcgtg attggtgagg
     4861 tcctcgactg tgacggtctt ttggtcaggg tcgatggcca gcacgttgtg gttcatctgc
     4921 acattagcgc ccagcttctg cagttcttca ggggagctgt agaagaggcc ttggggatcg
     4981 gccacttttc cgcccaggta gagggcgatg ccacaggaca ggaaggagat cacatcgttc
     5041 ctctcgtaga cggtgacctc ggcgtcaggg tgctcagcga ggatttgctt gatggcgaag
     5101 gtgccggcat gggtgcatcc gaccacggtg accttcatcc ccatggtggg gatccaagta
     5161 taagacaaaa gtgttgtgga attgctccag gcgatctgac ggttcactaa acgagctctg
     5221 cttttatagg cgcccaccgt acacgcctac ctcgacatac gttctctatc actgataggg
     5281 agtaaactgg atatacgttc tctatcactg atagggagta aactgtagat acgttctcta
     5341 tcactgatag ggagtaaact ggtcatacgt tctctatcac tgatagggag taaactcctt
     5401 atacgttctc tatcactgat agggagtaaa gtctgcatac gttctctatc actgataggg
     5461 agtaaactct tcatacgttc tctatcactg atagggagta aactcgagtg aagacgaaag
     5521 ggcctcatcg ataaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     5581 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     5641 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaggcgata ccgtcgagat
     5701 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
     5761 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     5821 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     5881 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     5941 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     6001 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     6061 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     6121 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     6181 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     6241 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     6301 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     6361 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     6421 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     6481 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     6541 ccaggtctga agatcagcgg ccggccgctt gctgtgcggt ggtcttactt ttgttttgct
     6601 cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac
     6661 aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg
     6721 atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat
     6781 taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt
     6841 tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa
     6901 tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg
     6961 tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc
     7021 gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct
     7081 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
     7141 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg
     7201 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt
     7261 acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc ccagtcccgc
     7321 ccaggccacg cctccctgga aagtccccag cggaaagtcc cttgtagcaa gctcgatatc
     7381 agcagttctt gaagtactcc ggatgcagct ctcgggccac gtgatgaaat gctaggcggc
     7441 tgtcaaacct ccactctaac acttctctct ccgggtcatc catcccatgc aggctcacag
     7501 ggtgtaacaa gctggtgttc tctcctttat tggcctcttc taccttatct ggctcaactg
     7561 gtactagctt gtagcaccat ccaaaggtca gtggatatct gacccctggc cctggtgtgt
     7621 agttctgcta atcagggaag tagccttgtg tgtggtagat ccacagatca aggatatctt
     7681 gtcttctttg ggagtgaatt agcccttcca actactaagt ttgtagtaca tatttaacaa
     7741 atacaatttc tttaaaatga aaataattca gaggaatcac aggtttagag taaatgaaac
     7801 cacaggtaat tggcagtggt aatagggtat ggggtgggaa gtttgggatg attttggtta
     7861 gcttgagtta tccagttgat ccagacatga taagatacat tgatgagttt ggacaaacca
     7921 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     7981 ttgtaaccat tataagctgc aataaacaag ttgatctccc gatccgtcga cgtcaggtgg
     8041 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8101 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8161 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8221 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8281 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8341 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8401 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8461 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8521 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8581 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8641 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8701 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8761 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8821 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8881 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8941 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9001 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9061 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9181 gatcaaagga tcttc
//
