LOCUS       sequence-467498-       13897 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses Eggerthella lenta DSM 2243 El Vadh with a cumate inducible
            promoter, without accessory genes (inactive), in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 13897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN12-Pct3-ElVadh(cHis6)-noAccs(DSM2243)
FEATURES             Location/Qualifiers
     source          1..13897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHPFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             5911..5928
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7521..7548
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7680..7707
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(8139..8990)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8977..9816)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(10327..11298)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12537..12624
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      12966..13360
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13523..13566)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            13662..13771
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaagga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatgac tcagctcacg ctcacgcgcc gcagcttcat gaaggcgatg
     2641 gcggtcaccg gtgccgctgc gtcgctcacg gcggtcgccg agccgatgcg ggcgcttgcc
     2701 gagggcgtcg atgccgatgc gggggaagtc aagcgcgttc gctcgtgctg ccgcgcttgc
     2761 ggcaaggtgg agtgcggcgt ttgggtaacc gtgcgcgacg gcaaggtcgt caaggtggaa
     2821 ggcgacgagt ccgcgccgca aagccgcggg cactgctgct cgaagtcgca gtcatcgatg
     2881 caggcgctat atcatcccga ccgccttcgt ttcccggtga agcgcacgaa ccctaagggc
     2941 gaggacgatc ccggttgggt gcgcatcacg ctggacgagg cgttcgagat atgcggcgag
     3001 aagctcaagg aagtgaagga gaagtacggc ggggagtcca tcttcgtgat gtgcggcacg
     3061 tcgcgcgtgt ggtcgctcgg gccttaccag ggcatgaagc agctgttcgg cacgccgaac
     3121 gcgcatttgg cctaccaggt gtgcaaaggc ccgcgccact tcggcggcat catgaccgac
     3181 gagatggggt ccccgtggat ggaggtggag gccgagccca gcgtgtacgt gcagtggggc
     3241 acggcgtgcg agtactccaa ctacgactcc acgaaccgca cggtcaccga cgtcgcgcat
     3301 cgtgcgtcta agcatatcgt ggtggatccg cgcgtcacgc ctttgggcaa ggaagccgac
     3361 atctggctgc cgcttcgtcc cggcaccgac ggcgcgctgg cgctgtcgtg gctcaactgg
     3421 atcatcgaaa acgaagctta cgatgacacg atggtgcgtc gctggtcgaa cgcgtcgttc
     3481 ctctacgtgg acgacaagcc cgagctgacg caaggctggt tggtggaggg caacggcggc
     3541 atcaacatga agacgaagct gctgaccgag gccgacctca aggaagatgg caagtaccag
     3601 cgcttcatgg tgtgggacga ggcgaacgag cgtctcacgt actgggatgc cgagcttggc
     3661 atgtgggagg gcgaggagca ccgcatcccc accaccggca catggatcga gcatccgtac
     3721 aagccgcttg ttgccgatgc atggctgccc gatcagtcga cgttcgccga tccggctacc
     3781 gagcccgacc gcttccccga cggtttcgag gaatgcaatc ccaaggggtt gccgaagcgc
     3841 ccatctctgc ttcctggtga ggtagaggta acgctcaaag acggaaccgt gcacaaggct
     3901 cgctctgtgt gggatgcgtt ccatcagatg accagcgagt acaccatgga gaaggcagag
     3961 gagatcaccg gcgtgccggc ggcgaagagc gaggaggctg tacgcgcctg gacgacgcgc
     4021 gtcaatccgc tgcatggcaa cggcggcatc cacttccagc tggccaccga ccagaacggc
     4081 aactccatcc agaacgtgcg tgcgctgcag atcctgtcgt gcatcaccgg caactccgac
     4141 gagccggccg gcaaccgcgg ctcgtcgaag gcgcagttcg acggcaatcc agggcgttcg
     4201 aacatgcagg ccggcgcgcc gtacggcgac gaggccaaga tgtgggacgg ccgcgacgtc
     4261 gacctcgaag ggctcgccgc gaagatgcag gatttcgtgc agtacctcat cgacaacgat
     4321 tcgcccctgg ccgagcgcta cggcaacaag gtgcccagcc atgaggaagc cctcgttatc
     4381 gccaagcgca tgggcggcgc attccgcacg tcgcaggcgt ggccgaaccc caagaccgtg
     4441 ttcgagcgac aagccaacga ggttgacgcc gagcgattcc ccctcaaccg ctactgggcg
     4501 cgctgggcgg acgcgaactc catctgggac ggctgcctgg acaacgacgt gccgtaccag
     4561 ttgcatgcgg gcgtgtgcca gtcgggcgac ttcatgaata tgggcaacat cgacgtggcg
     4621 tgggaggcca tgacgaagct ggacttcttc accgacatca acctgtggtt ctgcccgaac
     4681 aacggcaacg ccgacgtcat ctttccgtgc taccactggt tggaagttga cacgacgcgc
     4741 gtcagccagg gcgccggcgg gttcttcggc tgcgggtgcg ccgctgtcga ggcgccgggc
     4801 gagtgcatct acgatcccga ctggaacgtg ggcatgtaca aggccatggg cgtgccatgg
     4861 aacacgaagg acgagtcggc cgagccgtcc gagatcctca acttcggcga gaagaccgac
     4921 taccgttggc cgaaccgcag ccgcgtgctc aaggacaacg tggacgcgtg gaagacagcc
     4981 gagttccccg acggtcccac gtgggagcaa gcgaaggagc acttccagaa gaacggctgg
     5041 atggattgcc gcacgtggca tcccgagcgt tggggcacgt atcgccggca cgagatgggc
     5101 tggcgtcgcc agcagggcgg cttcaacctg ttcccgttgg tggacgacca tccgggcttc
     5161 atgacgccgt cgggcctcat tgaaatctgg tcgcttgtgt gcgaggcgta cttaggcgac
     5221 gaggacaagt tccccgtgta ccgcgaaccc acgaactcgc cggtgaccac gcccgagtac
     5281 ttcgacgccg cgaaggttgc cgacatcgat gagtcgaagc tgcgcaatat ggattatccc
     5341 gccagcctgc aggagcacgc cgatgcgacg ttcctcatga ccacgggcgc gcgccagccg
     5401 gtgtacttcc acagcgagca tcgccagctg ccgtggtgcc gcgagctgtg gcccgcgccg
     5461 cgcctggaga tgaacccgaa cgacgctgcg cgtctggggc tcgagcaggg cgactgggtg
     5521 tggatcgaga gcccgtgggg caaggtgcgc gaggtgctgg atctgtacta cggcatcagc
     5581 aagggcgtgg tgaacgcgaa ccacgcgtgg tggttccccg agatggacac ggccagccac
     5641 ggctacgagc tggtgaacat caactgcgtg atggatcctt acggccagga tgtggtgtgc
     5701 ggtgcagcaa cgatgcgctc ggttcccgtg ctggtttata aggctacggc ggaaaactcg
     5761 ccgttcggca acccggtgcc gtgcgatccg cagggtaacc cgtgcatctg cgatgcgggc
     5821 gatccgcggt tgaaggaatg gatgggaacg ggattgcgat ctcgcgttga aggcgaagag
     5881 gcgtggaccg gagagggggc gggcagcagc catcaccatc accatcacta gacgatgaca
     5941 cagtacgcaa tcatcaccga tttgaaccgg tgcgtcggat gccttgcgtg cacggtcgca
     6001 tgcaaagcgg tgaacaacgt gcccgtcggg gcgttttgga tcaagacgct gcgcgtgggt
     6061 ccgaatccga tcgagggcgg aagcggcgat tggcccgacg ttgagatgta tttcctgccc
     6121 atccaatgcc agcattgcgc cgatcccgag tgcgtgaagg tgtgtcccac gggcgcctcg
     6181 cataaggcag ccgacggcac cgtgcagatc gacaagtcga agtgcattgg ctgccagttc
     6241 tgcgcgatga gctgccccta caacgttcgc tacctcaacg aagaggagcg cgtggtggag
     6301 aagtgcacgc tgtgcgagca gaagatcgcc caaggcgagc ttccgcagtg cgtggcgcaa
     6361 tgcggctcgc gtgcgcgctt cttcggcgac ttggagcagg gcgtggacgg gttcgaggca
     6421 ccggctccgc ctcaagccct gtcgtgcgac tacgccgaaa tgcagcagac gcgcgtcacg
     6481 ctgaaggagt acgtcgaacc gtatacggaa gacgagatcc atcgtctgcc cgatgttgga
     6541 aatggcccgg agctcatgta cctgctgcgc tccgaccgaa agtggagggg gtagaccatg
     6601 gaactgcaat ggcctttgat cgtgtttacc accctggttg cctggtcggc ggggctgttc
     6661 gggacgcagg cgctgatggc ggtgttcggc gtcgggaaga aggcgcaggt gccggcttgg
     6721 gtctgctcgg ccgtgctgct ggcggccggc gggatcgcgg tgttcttcca tctcgagcac
     6781 tgggagcgga tattcaacgg gttcgggcac ctgacgagcg ggatcaccca ggaattgatc
     6841 gcgatcatcg tgcttgccgt ggtggctatc gtctacttgg tgctcatgcg caagtccgac
     6901 gacggcgcga gcgtgccgaa gtggctggcc tgggtcgccg tcgcgctgtc ggtggtgctg
     6961 gtcgccgtga tggcgcactc gtacacgatg gccgcccgcc ccgcctggga ctcggccctc
     7021 tggatcctct acgtcctcgg caacgcctgc gtgttgggcc ccgcgacgtt cgccctcctc
     7081 tccgccttgg ctgcaggggg accccgggac cagcccgccg agcgcgcagc cgacgccggg
     7141 gcccccgcgg gtaggacgcc cctggtaggc gcagccgcca acgccctcgc aaccctcgcc
     7201 ttcgccgcct tcctccagct ctccgccggc tccttcgccg acgtcggcct ctacttcgac
     7261 ccgacgcacc ccaccaaggc catggccgac gcagccgcga cggtcgcctc ccaggccccg
     7321 ctcctgtggc tgggcgccgt ggccgtcggc gcgatcgtcc ccctggccgc cgccttccta
     7381 ggcaggcgca cggggaactg gaagctctgg gccccggcag ccataatcgc cgccctcgtc
     7441 ggagccgtct gcatgcgcgt cgtcttctac aacctggggc tctcggtctt catgttctac
     7501 tagactagtc gactcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     7561 agagagaata taaaaagcca gattattaat ccggcttttt tattatttaa ttcctgttat
     7621 aaaaaaagga tcaattttga actctctccc aaagttgatc ccttaacgat ccctgcagga
     7681 gaaggccatc ctgacggatg gcctttttga agctttctag aatcgggggc ccccgggggg
     7741 attctagaac aatggaagtc ctcgtctcgc cctcgaatta gcccgcttaa tgagcgggct
     7801 ttttttgaat taattctcgc gagctggcac gacaggtttc ccgactggaa agcgggcagt
     7861 gagcgcaacg caattaatgt aagttagcgc gaattgcaag ctggccgacg cgctgggcta
     7921 cgtcttgctg gcgttcggga gcagaagagc atacatctgg aagcaaagcc aggaaagcgg
     7981 cctatggagc tgtgcggcag cgctcagtag gcaatttttc aaaatattgt taagcctttt
     8041 ctgagcatgg tatttttcat ggtattacca attagcagga aaataagcca ttgaatataa
     8101 aagataaaaa tgtcttgttt acaatagagt ggggggggtc agcctgccgc cttgggccgg
     8161 gtgatgtcgt acttgcccgc cgcgaactcg gttaccgtcc agcccagcgc gaccagctcc
     8221 ggcaacgcct cgcgcacccg ctggcggcgc ttgcgcatgg tcgaaccact ggcctctgac
     8281 ggccagacat agccgcacaa ggtatctatg gaagccttgc cggttttgcc ggggtcgatc
     8341 cagccacaca gccgctggtg cagcaggcgg gcggtttcgc tgtccagcgc ccgcacctcg
     8401 tccatgctga tgcgcacatg ctggccgcca cccatgacgg cctgcgcgat caaggggttc
     8461 agggccacgt acaggcgccc gtccgcctcg tcgctggcgt actccgacag cagccgaaac
     8521 ccctgccgct tgcggccatt ctgggcgatg atggatacct tccaaaggcg ctcgatgcag
     8581 tcctgtatgt gcttgagcgc cccaccacta tcgacctctg ccccgatttc ctttgccagc
     8641 gcccgatagc tacctttgac caccatggca tcagcggtga cggcctccca cttgggttcc
     8701 aggaacagcc ggagctgccg tccgccttcg gtcttgggtt ccgggccaag cactaggcca
     8761 ttaggcccag ccatggccac cagcccttgc aggatgcgca gatcatcagc gcccagcggc
     8821 tccgggccgc tgaactcgat ccgcttgccg tcgccgtagt catacgtcac gtccagcttg
     8881 ctgcgcttgc gctcgccccg cttgagggca cggaacaggc cgggggccag acagtgcgcc
     8941 gggtcgtgtc ggacgtggct gaggctgtgc ttgttcttag gcttcaccac ggggcacccc
     9001 cttgctcttg cgctgcctct ccagcacggc gggcttgagc accccgccgt catgccgcct
     9061 gaaccaccga tcagcgaacg gtgcgccata gttggccttg ctcacaccga agcggacgaa
     9121 gaaccggcgc tggtcgtcgt ccacacccca ttcctcggcc tcggcgctgg tcatgctcga
     9181 caggtaggac tgccagcgga tgttatcgac cagtaccgag ctgccccggc tggcctgctg
     9241 ctggtcgcct gcgcccatca tggccgcgcc cttgctggca tggtgcagga acacgataga
     9301 gcacccggta tcggcggcga tggcctccat gcgaccgatg acctgggcca tggggccgct
     9361 ggcgttttct tcctcgatgt ggaaccggcg cagcgtgtcc agcaccatca ggcggcggcc
     9421 ctcggcggcg cgcttgaggc cgtcgaacca ctccggggcc atgatgttgg gcaggctgcc
     9481 gatcagcggc tggatcagca ggccgtcagc cacggcttgc cgttcctcgg cgctgaggtg
     9541 cgccccaagg gcgtgcaggc ggtgatgaat ggcggtgggc gggtcttcgg cgggcaggta
     9601 gatcaccggg ccggtgggca gttcgcccac ctccagcaga tccggcccgc ctgcaatctg
     9661 tgcggccagt tgcagggcca gcatggattt accggcacca ccgggcgaca ccagcgcccc
     9721 gaccgtaccg gccaccatgt tgggcaaaac gtagtccagc ggtggcggcg ctgctgcgaa
     9781 cgcctccaga atattgatag gcttatgggt agccattgat tgcctccttt gcaggcagtt
     9841 ggtggttagg cgctggcggg gtcactaccc ccgccctgcg ccgctctgag ttcttccagg
     9901 cactcgcgca gcgcctcgta ttcgtcgtcg gtcagccaga acttgcgctg acgcatccct
     9961 ttggccttca tgcgctcggc atatcgcgct tggcgtacag cgtcagggct ggccagcagg
    10021 tcgccggtct gcttgtcctt ttggtctttc atatcagtca ccgagaaact tgccggggcc
    10081 gaaaggcttg tcttcgcgga acaaggacaa ggtgcagccg tcaaggttaa ggctggccat
    10141 atcagcgact gaaaagcggc cagcctcggc cttgtttgac gtataaccaa agccaccggg
    10201 caaccaatag cccttgtcac ttttgatcag gtagaccgac cctgaagcgc ttttttcgta
    10261 ttccataaaa cccccttctg tgcgtgagta ctcatagtat aacaggcgtg agtaccaacg
    10321 caagcactac atgctgaaat ctggcccgcc cctgtccatg cctcgctggc ggggtgccgg
    10381 tgcccgtgcc agctcggccc gcgcaagctg gacgctgggc agacccatga ccttgctgac
    10441 ggtgcgctcg atgtaatccg cttcgtggcc gggcttgcgc tctgccagcg ctgggctggc
    10501 ctcggccatg gccttgccga tttcctcggc actgcggccc cggctggcca gcttctgcgc
    10561 ggcgataaag tcgcacttgc tgaggtcatc accgaagcgc ttgaccagcc cggccatctc
    10621 gctgcggtac tcgtccagcg ccgtgcgccg gtggcggcta agctgccgct cggacagttc
    10681 gaggctggcc agcctgcggg ccttctcctg ctgccgctgg gcctgctcga tctgctggcc
    10741 agcctgctgc accagcgccg ggccagcggt ggcggtcttg cccttggatt cacgcagcag
    10801 cacccacggc tgataaccgg cgcgggtggt gtgcttgtcc ttgcggttgg tgaagcccgc
    10861 caagcggcca tagtggcggc tgtcggcgct ggccgggtcg gcgtcgtact cgctggccag
    10921 cgtccgggca atctgccccc gaagttcacc gcctgcggcg tcggccacct tgacccatgc
    10981 ctgatagttc ttcgggctgg tttccactac cagggcaggc tcccggccct cggctttcat
    11041 gtcatccagg tcaaactcgc tgaggtcgtc caccagcacc agaccatgcc gctcctgctc
    11101 ggcgggcctg atatacacgt cattgccctg ggcattcatc cgcttgagcc atggcgtgtt
    11161 ctggagcact tcggcggctg accattcccg gttcatcatc tggccggtgg tggcgtccct
    11221 gacgccgata tcgaagcgct cacagcccat ggccttgagc tgtcggccta tggcctgcaa
    11281 agtcctgtcg ttcttcatcg ggccaccaag cgcagccaga tcgagccgtc ctcggttgtc
    11341 agtggcgtca ggtcgagcaa gagcaacgat gcgatcagca gcaccaccgt aggcatcatg
    11401 gaagccagca tcacggttag ccatagcttc cagtgccacc cccgcgacgc gctccgggcg
    11461 ctctgcgcgg cgctgctcac ctcggcggct acctcccgca actctttggc cagctccacc
    11521 catgccgccc ctgtctggcg ctgggctttc agccactccg ccgcctgcgc ctcgctggcc
    11581 tgctgggtct ggctcatgac ctgccgggct tcgtcggcca gtgtcgccat gctctgggcc
    11641 agcggttcga tctgctccgc taactcgttg atgcctctgg atttcttcac tctgtcgatt
    11701 gcgttcatgg tctattgcct cccggtattc ctgtaagtcg atgatctggg cgttggcggt
    11761 gtcgatgttc agggccacgt ctgcccggtc ggtgcggatg ccccggcctt ccatctccac
    11821 cacgttcggc cccaggtgaa caccgggcag gcgctcgatg ccctgcgcct caagtgttct
    11881 gtggtcaatg cgggcgtcgt ggcctgcccg ctctaatgcc cggttggcat ggtcggccca
    11941 tgcctcgcgg gtctgctcaa gccatgcctt gggcttgagc gcttcggtct tctgtgcccc
    12001 gcccttctcc ggggtcttgc cgttgtaccg cttgaaccac tgagcggcgg gccgctcgat
    12061 gccgtcattg atccgctcgg agatcatcag gtggcagtgc gggttctcgc cgccaccggc
    12121 atggatggcc agcgtatacg gcaggcgctc ggcaccggtc aggtgctggg cgaactcgga
    12181 cgccagcgcc ttctgctggt cgagggtcag ctcgaccggc agggcaaatt cgacctcctt
    12241 gaacagccgc ccattggcgc gttcatacag gtcggcagca tcccagtagt cggcgggccg
    12301 ctcgacgaac tccggcatgt gcccggattc ggcgtgcaag acttcatcca tgtcgcgggc
    12361 atacttgcct tcgcgctgga tgaagtcggc cttggccctg gccgattggc cgcccgacct
    12421 gctgccggtt ttcgccgtaa ggtgataaat cgccatgctg cctcgctgtt gcttttgctt
    12481 ttcggctcca tgcaatggcc ctcggagagc gcaccgcccg aagggtggcc gttaggccag
    12541 tttctcgaag agaaaccggt aagtgcgccc tcccctacaa agtagggtcg ggattgccgc
    12601 cgctgtgcct ccatgatagc ctacgagaca gcacattaac aatggggtgt caagatggtt
    12661 aaggggagca acaaggcggc ggatcggctg gccaagctcg aagaacaacg agcgcgaatc
    12721 aatgccgaaa ttcagcgggt gcgggcaagg gaacagcagc aagagcgcaa gaacgaaaca
    12781 aggcgcaagg tgctggtggg ggccatgatt ttggccaagg tgaacagcag cgagtggccg
    12841 gaggatcggc tcatggcggc aatggatgcg taccttgaac gcgaccacga ccgcgccttg
    12901 ttcggtctgc cgccacgcca gaaggatgag ccgggctgaa tgatcgaccg agacaggccc
    12961 tgcggggctg cacacgcgcc cccacccttc gggtaggggg aaaggccgct aaagcggcta
    13021 aaagcgctcc agcgtatttc tgcggggttt ggtgtggggt ttagcgggct ttgcccgcct
    13081 ttccccctgc cgcgcagcgg tggggcggtg tgtagcctag cgcagcgaat agaccagcta
    13141 tccggcctct ggccgggcat attgggcaag ggcagcagcg ccccacaagg gcgctgataa
    13201 ccgcgcctag tggattattc ttagataatc atggatggat ttttccaaca ccccgccagc
    13261 ccccgcccct gctgggtttg caggtttggg ggcgtgacag ttattgcagg ggttcgtgac
    13321 agttattgca ggggggcgtg acagttattg caggggttcg tgacagttag tacgggagtg
    13381 acgggcactg gctggcaatg tctagcaacg gcaggcattt cggctgaggg taaaagaact
    13441 ttccgctaag cgatagactg tatgtaaaca cagtattgca aggacgcgga acatgcctca
    13501 tgtggcggcc ctcgcgagaa ttaattcaga taaaaaaaat ccttagcttt cgctaaggat
    13561 gatttctagc gatgaccctg ctgattggtt cgctgaccgt agactggttg gcttggtttc
    13621 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
    13681 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
    13741 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
    13801 gtctacacga accctttggc aaaatcctgt atatcgtgcg acatcgcgcg cgcgcgcgcg
    13861 gatgctagcg gcgcgccaaa aaaaagcccg ctcatta
//
