LOCUS       sequence-467551-        8456 bp    DNA     circular SYN 15-APR-2025
DEFINITION  equimolar expression of ymBaojin and mCherry in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Teusink B
  TITLE     Expression of FPs in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDRF1 ymBaojin-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    812..836
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             843..1544
                     /codon_start=1
                     /product="bright and photostable monomeric green 
                     fluorescent protein derived from StayGold (Zhang et al., 
                     2024)"
                     /label=mBaoJin
                     /label=mStayGold
                     /note="/vntifkey=21"
                     /translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
                     LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
                     EAHLKGMDELYK"
     CDS             1551..1604
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1620..1629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1626..2336
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1771..1789)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2039..2058
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2346..2370)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2432..2451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2433..2451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2599..2786
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2654..2678)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2798..2815)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2798..2814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2807..2829)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3250..4596
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4707..5510)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5511..5726)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5751..5769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5837..5941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5942..6010,6011..6802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6160..6179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6973..7561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7462..7481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7715..7732
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7849..7870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7885..7890,7891..7908,7909..7915)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7923..7939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7928..7950
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7947..7963
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7947..7963
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7993..8448)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8130..8151)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8342..8361
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttttttt tttttttacc tctgtggaaa ttgttactct cacactcttt
      421 agttcgtttg tttgttttgt ttattccaat tatgaccggt gacgaaacgt ggtcgatggt
      481 gggtaccgct tatgctcccc tccattagtt tcgattatat aaaaaggcca aatattgtat
      541 tattttcaaa tgtcctatca ttatcgtcta acatctaatt tctcttaaat tttttctctt
      601 tctttcctat aacaccaata gtgaaaatct ttttttcttc tatatctaca aaaacttttt
      661 ttttctatca acctcgttga taaatttttt ctttaacaat cgttaataat taattaattg
      721 gaaaataacc attttttctc tcttttatac acacattcaa aagaaagaaa aaaaatatac
      781 cccagcctcg actagtgaat tcgcggccat cacaagtttg tacaaaaaag caggctgcta
      841 gcatggtttc taagggtgaa gaagaaaaca tggcttctac tccattcaag ttccaattga
      901 agggtactat taacggtaag tctttcactg ttgaaggtga aggtgaaggt aactctcatg
      961 aaggttctca caagggtaag tacgtttgta cttctggtaa gttgccaatg tcttgggctg
     1021 ctttgggtac tactttcggt tacggtatga agtactacac taagtaccca tctggtttaa
     1081 agaactggtt cagagaagtt atgccaggtg gtttcactta cgatagacat attcaataca
     1141 agggtgatgg ttctattcat gctaagcatc aacatttcat gaagaacggt acttaccata
     1201 acattgttga attcactggt caagatttca aggaaaattc tccagttttg accggtgata
     1261 tgaacgtttc tttgccaaat gaagttccac aaattccaag agatgatggt gttgaatgtc
     1321 cagttacttt attgtaccca ttgttgtctg ataagtctaa gtatgttgaa gctcatcaat
     1381 acactatttg taagccattg cataaccaac cagctccaga tgtcccatac cattggatta
     1441 gaaagcaata cactcaatct aaggatgatg ctgaagaaag agatcatatt tgtcaatctg
     1501 aaactttaga agctcatttg aagggtatgg atgaattgta caagtccgga gagggcagag
     1561 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctggggat ccaccggtcg
     1621 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     1681 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1741 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     1801 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     1861 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     1921 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     1981 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     2041 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     2101 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     2161 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     2221 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2281 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaagcg
     2341 gccgcaccca gctttcttgt acaaagtggt gtagttaatt catgatggcc gctgcaggtc
     2401 gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcggat
     2461 ccagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg
     2521 tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt
     2581 gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
     2641 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     2701 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
     2761 gctcttattg accacacctc taccggcatg ccaattcact ggccgtcgtt ttacaacgtc
     2821 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     2881 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     2941 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3001 accgcataat cggatcgtac ttgttaccca tcattgaatt ttgaacatcc gaacctggaa
     3061 gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt ctagcgcttt
     3121 acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat tgcataggta
     3181 atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact tcaatagcat
     3241 atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg
     3301 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa
     3361 gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga
     3421 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     3481 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
     3541 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
     3601 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
     3661 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
     3721 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
     3781 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
     3841 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
     3901 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
     3961 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
     4021 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
     4081 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
     4141 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt
     4201 caaaagcgct ctgaagttcc tatactttct agctagagaa taggaacttc ggaataggaa
     4261 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     4321 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     4381 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     4441 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     4501 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     4561 atccttcaat gctatcattt cctttgatat tggatcgatc cgatgataag ctgtcaaaca
     4621 tgagaattgg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata
     4681 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct
     4741 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat
     4801 agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta
     4861 tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac
     4921 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa
     4981 tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag
     5041 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct
     5101 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg
     5161 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca
     5221 atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt
     5281 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt
     5341 aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca
     5401 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca
     5461 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt
     5521 cgtttcctgc atgtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt
     5581 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
     5641 tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa
     5701 gaataaaaaa aaaatgatga attgaaaagc taattcttga agacgaaagg gcctcgtgat
     5761 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5821 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5881 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5941 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6001 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6061 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6121 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6181 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6241 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6301 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6361 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6421 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6481 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6541 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6601 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6661 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6721 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6781 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6841 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6901 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7561 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7621 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7681 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7741 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7801 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7861 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7921 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     7981 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     8041 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     8101 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     8161 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     8221 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     8281 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     8341 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     8401 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgc
//
