LOCUS       sequence-467797-       10001 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Optimized thiosulfate sensor with fluorescent reporter (GFP), 
            constitutive mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 680 to 714)
  AUTHORS   Buss MT, Zhu L, Kwon JH, Tabor JJ, Shapiro MG
  TITLE     Probiotic acoustic biosensors for noninvasive imaging of gut 
            inflammation
  JOURNAL   bioRxiv 2024.09.23.614598
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.09.23.614598"
COMMENT     Sequence Label: MB 45t3 thsS(t3)R-sfGFP_mCherry
FEATURES             Location/Qualifiers
     source          1..10001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(93..638)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(173..192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        680..714
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             724..735
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             743..1453
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(888..906)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1156..1175
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2730..2764
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     terminator      4600..4671
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4687..4714
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     4781..4799
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4820..4854
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     terminator      5518..5589
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5605..5632
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             6098..6109
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6116..6829
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6852..6869)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7022..7108
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7200..7227
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        7246..7337
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7795..8250
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7882..7901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8092..8113
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8806..9465)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWCEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9380..9399
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(9466..9568)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(9960..9977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
       61 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
      121 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
      181 caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
      241 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
      301 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
      361 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
      421 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
      481 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
      541 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
      601 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga
      661 taaaatcgcc agatatcgat tgacggctag ctcagtccta ggtacagtgc tagcgaattc
      721 attaaagagg agaaaggtac ccatggtgag caagggcgag gaggataaca tggccatcat
      781 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      841 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      901 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      961 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     1021 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     1081 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     1141 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     1201 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     1261 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     1321 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     1381 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     1441 gctgtacaag taagctttct aactaaaaag gcctcccaaa tcggggggcc ttttttattg
     1501 ataacaaaac gctcgtctag ggttatttcg atgaaaggag agagtactaa tgccaattgc
     1561 aataggaaat aaacggttac ccgttacgtt ggatgaaaag agacaaaaag aattgcagca
     1621 actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
     1681 gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac gtcacttttt
     1741 atcctaaaaa ctaaaagtga tagcactttt aattataaga agttagaata ttaatcattt
     1801 gcttaattgt acaatataat gtacaattgt tttatagaaa taaataaggg gtgaaaggaa
     1861 tggaagcagt agcttattca aatttccgcc aaaatttacg tagttatatg aaacaagtta
     1921 atgaggatgc tgaaacactt attgtaacaa gtaaagatgt agaagataca gttgttgtat
     1981 tatcaaaaag agattatgat tctatgcaag aaacgttgag aacactttct aataattacg
     2041 tcatggaaaa aattcgtcga ggagatgaac aattctccaa aggtgcattt aaaacacatg
     2101 acttaatcga ggttgaatct gatgattaag gcttggtctg atgatgcttg ggatgattat
     2161 ctttattggc atgagcaagg aaacaaaagc aatataaaaa agattaacaa gttaataaaa
     2221 gatatcgatc gttccccctt tgctggatta ggaaaacctg agccattaaa gcatgattta
     2281 tctggaaaat ggtccagaag aattacagat gaacatagac tgatatatag agttgaaaat
     2341 gaaacgatat ttatttattc tgcaaaagat cactattaac caatcggaag taaggaaagg
     2401 gtcagaaact taaaagtttt tgatccttat tttatttacc ctagtcattt aaaaagctaa
     2461 tatagcttag tgttgattgt tattaatgaa tgtgtttgtt acgcgtatta cggatataag
     2521 gttagtaaaa tcatttctaa agttgaggaa aagtaaatat aaatggctta aatttcaaca
     2581 atttgaagtt gaatagatat gttataatac tattgtagtg tgggatgtta gttactaaag
     2641 gatgacgctt atatatatga ctgaatagaa taagcaatag gtttaataat ctattttaaa
     2701 ttttttgtac tagttttagt taattacggt tgacagctag ctcagtccta ggtattgtgc
     2761 tagcctagta tcgatctcca taactatcct atagatcatg tcccgcctgc tgctgtgtat
     2821 ctgtgttctg ctgttctctt ctgtggcgtg gtctaaaccg cagcagtttt atgtgggcgt
     2881 actggctaac tggggtcatc agcaagccgt tgaacgttgg accccgatga tggagtatct
     2941 gaacgaacac gtgccggacg cggaatttca cgtctacccg ggcaacttca aagcactgaa
     3001 cctggcaatg gaactgggcc agattcagtt cattatcact aacccgggcc aatatctgta
     3061 cctgagcaat cagtacccgc tgtcttggct ggcgaccatg cgttctaagc gtcacgatgg
     3121 taccacttct gcgatcggtt ccgccattat tgtccgcgcg gacagcgact accgcaccct
     3181 gtacgacctg aaaggtaaag tggtggctgc gtccgacccg catgctctgg gtggctacca
     3241 agcgaccgtc ggtctgatgc attccctggg catggatccg gacaccttct tcggtgaaac
     3301 caagtttctg ggctttccac tggatccgct gctgtaccaa gttcgtgatg gcaacgttga
     3361 cgcggccatt accccactgt gcactctgga ggacatggtt gcacgcggcg tactgaaatc
     3421 ttccgatttt cgtgtgctga accctagccg cccggatggt gtagaatgcc agtgctctac
     3481 caccctgtac ccgaactggt ctttcgctgc gactgagtct gtatccaccg aactgtctaa
     3541 agaaatcacg caggcactgc tggaactgcc atccgacagc ccggcagcta tcaaagcgca
     3601 actgaccggc tggaccagcc cgatctccca actggcggta atcaaactgt tccaagagct
     3661 gcacgtaaaa accccggact ctagccgttg ggaagccgtt aagaagtggc tggaagaaaa
     3721 ccgtcactgg ggtatcctgt ctgttctggt gttcatcatt gcaacgctgt atcacctgtg
     3781 gattgaatac cgcttccacc aaaaaagctc ctctctgatc gaatctgaac gtcagctgaa
     3841 acagcatgct gttgccctgg aacgtctgca atctgctagc atcgttggtg aaattggtgc
     3901 gggtctggcc cacgagatta atcagccgat cgctgcaatt acctcttatt ctgaaggtgg
     3961 catcatgcgc ctgcaaggta aagaacaggc ggatacggat agctgcatcg aactgctgga
     4021 aaaaatccac aaacagagca ctcgcgcagg cgaagtggtg caccgcatcc gtggtctgct
     4081 gaaacgtcgt gaagcggtga tggtagatgt taacatcctg accctggtgg aagaatccat
     4141 cagcctgctg cgtctggagc tggcacgtcg cgaaatccag atcaacactc agatcaaagg
     4201 tgaaccgttc ttcattactg ccgaccgcgt tggcctgctg caagttctga ttaacctgat
     4261 caaaaactcc ctggacgcga tcgctgaatc tgataatgcc cgttctggta aaatcaacat
     4321 cgaactggac tttaaagagt accaggtaaa cgtctccatc atcgataacg gtccgggcct
     4381 ggcgatggat tctgacactc tgatggctac gttttacact accaaaatgg atggcctggg
     4441 cctgggtctg gcagtctgcc gcgaagttat cagcaaccac gacggccaca tcctgctgtc
     4501 caaccgtgac gacggcgttc tgggctgtgt ggcaaccctg aatctgaaaa aacgcggttc
     4561 tgaagtgccg atcgaagtct aataatctag accaggcatc aaataaaacg aaaggctcag
     4621 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     4681 cacactggct caccttcggg tgggcctttc tgcgtttata ggtacccggg gatcctctag
     4741 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4801 ctttcgtctt cacctcgagt ttacggctag ctcagtccta ggtactatgc tagcagaaat
     4861 ataaagaacg atctatttat ccgcgtacat gcagcagcaa atcaacggcc cggtctacct
     4921 ggtggatgat gatgaagcca ttatcgactc catcgatttt ttgatggagg gctacggtta
     4981 caaactgaac tcgtttaact gcggcgatcg ctttttggca gaagtcgatc tcacccaggc
     5041 aggatgtgta attctggatg cgcgtatgcc aggcttaact ggtcctcagg tgcaacagct
     5101 gctgagcgac gcgaaaagcc cgcttgcggt catcttcctg accggccatg gcgatgttcc
     5161 gatggcggtt gatgcgttca aaaatggcgc gttcgatttc tttcaaaaac ctgtgccggg
     5221 tagcttgctc agtcagtcaa ttgccaaagg cttgacttat tcaatcgatc aacatctgaa
     5281 acgtactaac caagcgttaa tcgacacgct ctcggaacgc gaagctcaaa tttttcaact
     5341 ggtgattgca ggcaacacca acaaacagat ggctaacgag ctttgcgtgg ctattcgtac
     5401 cattgaggtt caccgtagca aactgatgac caaactgggt gttaacaacc tggctgaact
     5461 ggttaaactg gcgccgctgc tggcacataa atccgaataa taatctagac caggcatcaa
     5521 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     5581 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatagt
     5641 aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc
     5701 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctggttagct agtcattcaa
     5761 gcattattat gctgtttttt gaagtgaatg tgcggccatc tagccgcaca ttttgcatct
     5821 aaaacatgca gtcatcagca aaataataaa cttttcccca atatgtggtt taccacaatt
     5881 tacaggaatt cactcctgtg gtggtgcaaa tttgaactgt gaattgcttc acaaacgccg
     5941 ctatcgcaat gtcagtatgt ggtttaccac aatatctaat atcactctgc tcaataacaa
     6001 tgatgaaaac cttaggaaga agttaattgt gttaaacagt taactagggg ctttatctaa
     6061 cgctctccta aggacaactg tcattgggag atttaacaaa gaggagaaat actagatgcg
     6121 taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt
     6181 caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact
     6241 gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga ctctggtaac
     6301 gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga
     6361 cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga
     6421 tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg
     6481 cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga
     6541 atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa
     6601 agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta
     6661 ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag
     6721 cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga
     6781 gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat gatgagagag
     6841 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
     6901 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
     6961 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
     7021 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     7081 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     7141 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca
     7201 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct
     7261 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7321 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7381 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7441 aagatcagtt gggtgcagca aactattaac tggcgaacta cttactctag cttcccggca
     7501 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7561 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7621 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7681 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7741 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttacgcgc
     7801 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7861 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7921 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7981 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     8041 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8101 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     8161 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8221 attttaacaa aatattaacg tttacaattt aaaaggatct aggtgaagat cctttttgat
     8281 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8341 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8401 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8461 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8521 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8581 atcctgttac cagtcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata
     8641 aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga
     8701 ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc
     8761 gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg
     8821 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     8881 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     8941 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     9001 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     9061 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     9121 ctgccggaaa tcgtcgtggt attcacacca gagcgatgaa aacgtttcag tttgctcatg
     9181 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     9241 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     9301 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     9361 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     9421 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     9481 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     9541 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     9601 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     9661 aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat
     9721 gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag
     9781 ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg
     9841 tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg
     9901 agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc
     9961 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc g
//
