LOCUS       sequence-467799-        9985 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Optimized tetrathionate sensor with fluorescent reporter (GFP), 
            constitutive mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 42 to 76)
  AUTHORS   Buss MT, Zhu L, Kwon JH, Tabor JJ, Shapiro MG
  TITLE     Probiotic acoustic biosensors for noninvasive imaging of gut 
            inflammation
  JOURNAL   bioRxiv 2024.09.23.614598
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.09.23.614598"
COMMENT     Sequence Label: MB 101A ttrSR(m13)-sfGFP_mCherry
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..76
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             86..97
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             105..815
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(250..268)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     518..537
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2093..2127
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
     regulatory      3255..3264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      4196..4267
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4283..4310
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     4377..4395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4416..4450
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     terminator      5121..5192
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5208..5235
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             5444..5455
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5462..6175
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6198..6215)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6368..6454
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6546..6573
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6592..6683
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7141..7596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7228..7247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7438..7459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8152..8811)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWCEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8726..8745
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8812..8914)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(9306..9323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9440..9985)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9520..9539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcatc ttattaatca gataaaatcg ccagatatcg attgacggct agctcagtcc
       61 taggtacagt gctagcgaat tcattaaaga ggagaaaggt acccatggtg agcaagggcg
      121 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      181 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      241 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
      301 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      361 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      421 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      481 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      541 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
      601 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
      661 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
      721 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
      781 actccaccgg cggcatggac gagctgtaca agtaagcttt ctaactaaaa aggcctccca
      841 aatcgggggg ccttttttat tgataacaaa acgctcgtct agggttattt cgatgaaagg
      901 agagagtact aatgccaatt gcaataggaa ataaacggtt acccgttacg ttggatgaaa
      961 agagacaaaa agaattgcag caactaaagc agaagtacgg caaaagtgaa tccaggatta
     1021 tgtgtattgc gttagattta ttgattgccc aagaaaaagc aggatttgag gtaccagcac
     1081 tcaaaaagtg acgtcacttt ttatcctaaa aactaaaagt gatagcactt ttaattataa
     1141 gaagttagaa tattaatcat ttgcttaatt gtacaatata atgtacaatt gttttataga
     1201 aataaataag gggtgaaagg aatggaagca gtagcttatt caaatttccg ccaaaattta
     1261 cgtagttata tgaaacaagt taatgaggat gctgaaacac ttattgtaac aagtaaagat
     1321 gtagaagata cagttgttgt attatcaaaa agagattatg attctatgca agaaacgttg
     1381 agaacacttt ctaataatta cgtcatggaa aaaattcgtc gaggagatga acaattctcc
     1441 aaaggtgcat ttaaaacaca tgacttaatc gaggttgaat ctgatgatta aggcttggtc
     1501 tgatgatgct tgggatgatt atctttattg gcatgagcaa ggaaacaaaa gcaatataaa
     1561 aaagattaac aagttaataa aagatatcga tcgttccccc tttgctggat taggaaaacc
     1621 tgagccatta aagcatgatt tatctggaaa atggtccaga agaattacag atgaacatag
     1681 actgatatat agagttgaaa atgaaacgat atttatttat tctgcaaaag atcactatta
     1741 accaatcgga agtaaggaaa gggtcagaaa cttaaaagtt tttgatcctt attttattta
     1801 ccctagtcat ttaaaaagct aatatagctt agtgttgatt gttattaatg aatgtgtttg
     1861 ttacgcgtat tacggatata aggttagtaa aatcatttct aaagttgagg aaaagtaaat
     1921 ataaatggct taaatttcaa caatttgaag ttgaatagat atgttataat actattgtag
     1981 tgtgggatgt tagttactaa aggatgacgc ttatatatat gactgaatag aataagcaat
     2041 aggtttaata atctatttta aattttttgt actagtttta gttaattagc ggtttacagc
     2101 tagctcagtc ctagggactg tgctagcata atcattaaca acatcttata tcaaatttta
     2161 atatgttttc ttccggcatg agcctgaaat ggcctctgag catccagggt tccatcgaac
     2221 cgaaaatgac tccgcgcatc atcttccgca tggccctgcg cactaaagtg ttcatcaccc
     2281 tgctggcttg cctgtttgct tctgccaacg tattcgcggt ggaaccgcag aaccagtccg
     2341 cgccttccga tgtgatggac gctgaggtgg ttgctccggt tgcgacggag gaaccgggtt
     2401 accaggtcgt tgatgttggt gtgctggcga ttcgcggtta taaagctacc atcaaccgtt
     2461 ggcagccact gatgggttgg ctggagactc agatcccgaa ctcctacttc cgcctgcacc
     2521 cgctgtctct ggacgagctg gcaaagggtg tagaaaccca gggtctggac ttcgtaatca
     2581 ccaatccagg ccagtccgta ctgctggcgc gtcagtattc tctgacgtgg ctggcgaccc
     2641 tgcgctctcc gctgaacaac ggtgctgcga tgcaggtggg ttccgcgctg gtagttcgtg
     2701 ccgacagccc gtaccagacc ctgaccgatc tgaaaggccg tcgtctgggc atcgtcagca
     2761 aaaacgcatt cggtggttat ctgacgctgg tatatgaggc acagctgaaa ggcatcgatc
     2821 tgccgcgctt tgttggtgaa atcatcccac tgggcttccc gctggataac ctgctgtatc
     2881 agctggatga tcagaaatct ggtaatgacg ccgcaaaaga caatcgtctg gatgccaccg
     2941 ttgtaccggt atgccaactg gaacagatgc aggccgaagg tctgatcaac atcggccact
     3001 atcgcgttct ggacaaccag accccggtag gtttccactg tcaagtaagc actcgtctgt
     3061 atccgaactg gtctatggct aaaactaacc gtgccagcca gtccctggca aaatctgtga
     3121 cccaggctct gctggctctg ccggaagatc acctggcagc gaaaagctct gactctgcag
     3181 gttggaccac tgcagtgtcc cagctggcga tcgaccagct gctgaaagac ctggacatgc
     3241 atccactgca gaccccatgg tggcagcgcg cttggcagtg ggtaaaactg caccagcagt
     3301 gggcgtggtt cattctgggt atcctggtcc tgctgaacgc ataccatttc tggctggaat
     3361 accgtttctc ccgtcgtggt cgtgaactga tcaacaccca gcgccagctg aacgaaaacc
     3421 gtgctctgct ggagcacgca caacgtatcg ccatcgctgg cgaactgggt gctagcctgt
     3481 cccatgaact gaaccagccg ctggctgcaa ttggtcacta ttgccatggt gcggaagtgc
     3541 gtctgcagcg tggtacttct cctgaagaac tgcagtctgt tctgaccctg atccagcagg
     3601 aagttacgcg cgccgactct atcatcagcc gtctgcgtaa cctgctgaaa aaacgcccgg
     3661 ttagcaaaca ggctctgtat ctgcacgaac tggtaaacga aacggtgccg ctgctggcgt
     3721 acgaattcga acagcaccag atcaacctgg cggtaaacgt cagcggtgaa ccttacctgc
     3781 aatctctgga cgaggtcggt atgcagcagc tgctgctgaa cctgctgaaa aacgcgtttg
     3841 acgcgtgcgt tcagcgcctg gaactggaat cctccggcac tgaacagagc ggtatgcgta
     3901 aaccgtatgt tcctacgatc gatattgatc tgcgctacca ggagcgtact ctgctgctga
     3961 ctgtgacgga taacggcact ggtctgaccg aggagactag cctgctgatg caggccttct
     4021 attccaccaa atctgaaggc ctgggcctgg gtctggttat ttgccgtgat atcgctgagt
     4081 cccacggtgg caccttctct ctggaatccg ccatgggtgg tggctgccag gctcaggtgg
     4141 ccatcccgcg caagccggaa ccgaatggcg ttctgtaata atctagacca ggcatcaaat
     4201 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     4261 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggta
     4321 cccggggatc ctctagcgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4381 ggcgtatcac gaggcccttt cgtcttcacc tcgagttgat agctagctca gtcctaggta
     4441 ttgtgctagc accaaatata gagaatcaca aggatctatt caatgagcct ggcgctgccg
     4501 gtgtacctga ttgacgacga tgactccgtt cgccgttccc tgcgtttcat gctggaaagc
     4561 tacggcctga aaatcactga cttcgattct gctgaagcgt tcttcaccgc ggtagacctg
     4621 accctgccgg gttgcgcact ggtggacgta cgtatgccgg gcctgagcgg cccacagctg
     4681 cacctggaac tggtgtctaa gaacagcccg ctggccgtga tctatctgac cggtcacggc
     4741 gacgttccga tggcggttga agcgctgaaa ctgggtgcgg tagatttctt tcagaaacct
     4801 gcagacggcg ctaaactggc tgaagctgtg gtcaaagccc tggaacacgc gaaaacccac
     4861 taccaagaca accagtacct ggaaacctat caggctctga ccccacgtga gcgcgaaatc
     4921 ctgaacctga ttgcgcaagg tctgaaaaac caggaaatcg cggacaacct gtgcattgcg
     4981 atgcgcaccg tagaaatcca ccgtgcgaac ctgatgaaag gtatgcaggt gggcagcctg
     5041 gcagagatga tgctgattta ctctcgtatc gcggagcgtc tgcgcctgga agataaccgt
     5101 taataatcta gaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     5161 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     5221 gtgggccttt ctgcgtttat agtaaatcac tgcataattc gtgtcgctca aggcgcactc
     5281 ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat
     5341 gagctggtta gctagtcaat atttgttgcg ctagatcaaa tccacgcctg atatgtggaa
     5401 aaccactata gttatgccgc ctcgcctttt acagaatgcc tgtaaagagg agaaatacta
     5461 gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg
     5521 tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg
     5581 taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct
     5641 ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca
     5701 gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt
     5761 taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt
     5821 aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc tgggccataa
     5881 gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg
     5941 cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga
     6001 tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta
     6061 tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct
     6121 gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaaatgatg
     6181 agagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
     6241 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
     6301 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
     6361 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     6421 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     6481 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
     6541 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
     6601 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6661 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6721 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6781 atgctgaaga tcagttgggt gcagcaaact attaactggc gaactactta ctctagcttc
     6841 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6901 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6961 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7021 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7081 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7321 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     7381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7441 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     7501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7561 acgcgaattt taacaaaata ttaacgttta caatttaaaa ggatctaggt gaagatcctt
     7621 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7681 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7741 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7801 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7861 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7921 ctgctaatcc tgttaccagt caggcatttg agaagcacac ggtcacactg cttccggtag
     7981 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
     8041 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
     8101 tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
     8161 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     8221 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     8281 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     8341 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     8401 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     8461 tagaaactgc cggaaatcgt cgtggtattc acaccagagc gatgaaaacg tttcagtttg
     8521 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     8581 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     8641 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     8701 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     8761 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     8821 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     8881 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
     8941 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
     9001 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta
     9061 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct
     9121 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc
     9181 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
     9241 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
     9301 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
     9361 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
     9421 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     9481 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9541 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     9601 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     9661 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     9721 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
     9781 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
     9841 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
     9901 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
     9961 tacgcgcaga ccaaaacgat ctcaa
//
