LOCUS       sequence-467800-       27397 bp    DNA     circular UNA 15-APR-2025
DEFINITION  Optimized tetrathionate sensor with recombinase switch and acoustic 
            reporter genes (bARGSer).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 27397)
  AUTHORS   Buss MT, Zhu L, Kwon JH, Tabor JJ, Shapiro MG
  TITLE     Probiotic acoustic biosensors for noninvasive imaging of gut 
            inflammation
  JOURNAL   bioRxiv 2024.09.23.614598
REFERENCE   2  (bases 1 to 27397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.09.23.614598"
COMMENT     Sequence Label: MB 98w3 ttrSR(m13)-Bxb1_P7-bARGSer
FEATURES             Location/Qualifiers
     source          1..27397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1241..1275
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
     regulatory      2403..2412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      3344..3415
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3431..3458
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     3525..3543
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3564..3598
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     terminator      4269..4340
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4356..4383
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             4679..6178
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      complement(6556..6585)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     protein_bind    6637..6686
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    6741..6788
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     misc_RNA        6795..6844
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             15854..15865
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(22492..22503)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(23610..23627)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      23780..23866
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      23958..23985
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        24004..24095
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      24553..25008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(24640..24659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     24850..24871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(25564..26223)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWCEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     26138..26157
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(26224..26326)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(26718..26735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(26852..27397)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(26932..26951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcatc ttattaatca gataaaatag ggttatttcg atgaaaggag agagtactaa
       61 tgccaattgc aataggaaat aaacggttac ccgttacgtt ggatgaaaag agacaaaaag
      121 aattgcagca actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt
      181 tagatttatt gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac
      241 gtcacttttt atcctaaaaa ctaaaagtga tagcactttt aattataaga agttagaata
      301 ttaatcattt gcttaattgt acaatataat gtacaattgt tttatagaaa taaataaggg
      361 gtgaaaggaa tggaagcagt agcttattca aatttccgcc aaaatttacg tagttatatg
      421 aaacaagtta atgaggatgc tgaaacactt attgtaacaa gtaaagatgt agaagataca
      481 gttgttgtat tatcaaaaag agattatgat tctatgcaag aaacgttgag aacactttct
      541 aataattacg tcatggaaaa aattcgtcga ggagatgaac aattctccaa aggtgcattt
      601 aaaacacatg acttaatcga ggttgaatct gatgattaag gcttggtctg atgatgcttg
      661 ggatgattat ctttattggc atgagcaagg aaacaaaagc aatataaaaa agattaacaa
      721 gttaataaaa gatatcgatc gttccccctt tgctggatta ggaaaacctg agccattaaa
      781 gcatgattta tctggaaaat ggtccagaag aattacagat gaacatagac tgatatatag
      841 agttgaaaat gaaacgatat ttatttattc tgcaaaagat cactattaac caatcggaag
      901 taaggaaagg gtcagaaact taaaagtttt tgatccttat tttatttacc ctagtcattt
      961 aaaaagctaa tatagcttag tgttgattgt tattaatgaa tgtgtttgtt acgcgtatta
     1021 cggatataag gttagtaaaa tcatttctaa agttgaggaa aagtaaatat aaatggctta
     1081 aatttcaaca atttgaagtt gaatagatat gttataatac tattgtagtg tgggatgtta
     1141 gttactaaag gatgacgctt atatatatga ctgaatagaa taagcaatag gtttaataat
     1201 ctattttaaa ttttttgtac tagttttagt taattagcgg tttacagcta gctcagtcct
     1261 agggactgtg ctagcataat cattaacaac atcttatatc aaattttaat atgttttctt
     1321 ccggcatgag cctgaaatgg cctctgagca tccagggttc catcgaaccg aaaatgactc
     1381 cgcgcatcat cttccgcatg gccctgcgca ctaaagtgtt catcaccctg ctggcttgcc
     1441 tgtttgcttc tgccaacgta ttcgcggtgg aaccgcagaa ccagtccgcg ccttccgatg
     1501 tgatggacgc tgaggtggtt gctccggttg cgacggagga accgggttac caggtcgttg
     1561 atgttggtgt gctggcgatt cgcggttata aagctaccat caaccgttgg cagccactga
     1621 tgggttggct ggagactcag atcccgaact cctacttccg cctgcacccg ctgtctctgg
     1681 acgagctggc aaagggtgta gaaacccagg gtctggactt cgtaatcacc aatccaggcc
     1741 agtccgtact gctggcgcgt cagtattctc tgacgtggct ggcgaccctg cgctctccgc
     1801 tgaacaacgg tgctgcgatg caggtgggtt ccgcgctggt agttcgtgcc gacagcccgt
     1861 accagaccct gaccgatctg aaaggccgtc gtctgggcat cgtcagcaaa aacgcattcg
     1921 gtggttatct gacgctggta tatgaggcac agctgaaagg catcgatctg ccgcgctttg
     1981 ttggtgaaat catcccactg ggcttcccgc tggataacct gctgtatcag ctggatgatc
     2041 agaaatctgg taatgacgcc gcaaaagaca atcgtctgga tgccaccgtt gtaccggtat
     2101 gccaactgga acagatgcag gccgaaggtc tgatcaacat cggccactat cgcgttctgg
     2161 acaaccagac cccggtaggt ttccactgtc aagtaagcac tcgtctgtat ccgaactggt
     2221 ctatggctaa aactaaccgt gccagccagt ccctggcaaa atctgtgacc caggctctgc
     2281 tggctctgcc ggaagatcac ctggcagcga aaagctctga ctctgcaggt tggaccactg
     2341 cagtgtccca gctggcgatc gaccagctgc tgaaagacct ggacatgcat ccactgcaga
     2401 ccccatggtg gcagcgcgct tggcagtggg taaaactgca ccagcagtgg gcgtggttca
     2461 ttctgggtat cctggtcctg ctgaacgcat accatttctg gctggaatac cgtttctccc
     2521 gtcgtggtcg tgaactgatc aacacccagc gccagctgaa cgaaaaccgt gctctgctgg
     2581 agcacgcaca acgtatcgcc atcgctggcg aactgggtgc tagcctgtcc catgaactga
     2641 accagccgct ggctgcaatt ggtcactatt gccatggtgc ggaagtgcgt ctgcagcgtg
     2701 gtacttctcc tgaagaactg cagtctgttc tgaccctgat ccagcaggaa gttacgcgcg
     2761 ccgactctat catcagccgt ctgcgtaacc tgctgaaaaa acgcccggtt agcaaacagg
     2821 ctctgtatct gcacgaactg gtaaacgaaa cggtgccgct gctggcgtac gaattcgaac
     2881 agcaccagat caacctggcg gtaaacgtca gcggtgaacc ttacctgcaa tctctggacg
     2941 aggtcggtat gcagcagctg ctgctgaacc tgctgaaaaa cgcgtttgac gcgtgcgttc
     3001 agcgcctgga actggaatcc tccggcactg aacagagcgg tatgcgtaaa ccgtatgttc
     3061 ctacgatcga tattgatctg cgctaccagg agcgtactct gctgctgact gtgacggata
     3121 acggcactgg tctgaccgag gagactagcc tgctgatgca ggccttctat tccaccaaat
     3181 ctgaaggcct gggcctgggt ctggttattt gccgtgatat cgctgagtcc cacggtggca
     3241 ccttctctct ggaatccgcc atgggtggtg gctgccaggc tcaggtggcc atcccgcgca
     3301 agccggaacc gaatggcgtt ctgtaataat ctagaccagg catcaaataa aacgaaaggc
     3361 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     3421 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tataggtacc cggggatcct
     3481 ctagcgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3541 ggccctttcg tcttcacctc gagttgatag ctagctcagt cctaggtatt gtgctagcac
     3601 caaatataga gaatcacaag gatctattca atgagcctgg cgctgccggt gtacctgatt
     3661 gacgacgatg actccgttcg ccgttccctg cgtttcatgc tggaaagcta cggcctgaaa
     3721 atcactgact tcgattctgc tgaagcgttc ttcaccgcgg tagacctgac cctgccgggt
     3781 tgcgcactgg tggacgtacg tatgccgggc ctgagcggcc cacagctgca cctggaactg
     3841 gtgtctaaga acagcccgct ggccgtgatc tatctgaccg gtcacggcga cgttccgatg
     3901 gcggttgaag cgctgaaact gggtgcggta gatttctttc agaaacctgc agacggcgct
     3961 aaactggctg aagctgtggt caaagccctg gaacacgcga aaacccacta ccaagacaac
     4021 cagtacctgg aaacctatca ggctctgacc ccacgtgagc gcgaaatcct gaacctgatt
     4081 gcgcaaggtc tgaaaaacca ggaaatcgcg gacaacctgt gcattgcgat gcgcaccgta
     4141 gaaatccacc gtgcgaacct gatgaaaggt atgcaggtgg gcagcctggc agagatgatg
     4201 ctgatttact ctcgtatcgc ggagcgtctg cgcctggaag ataaccgtta ataatctaga
     4261 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     4321 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     4381 gcgtttatag taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
     4441 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctggttagc
     4501 tagtcaatat ttgttgcgct agatcaaatc cacgcctgat atgtggaaaa ccactatagt
     4561 tatgccgcct cgccttttac agaatgcctg tacgcactga ccaccatgaa ggtgacgctc
     4621 atgacttact tgctgaatct caggagttta tgaccttttt tttttagatc ctatcggtat
     4681 gagagccctg gtagtcatcc gcctgtcccg cgtcaccgat gctacgactt caccggagcg
     4741 tcagctggag tcttgccagc agctctgcgc ccagcgcggc tgggacgtcg tcggggtagc
     4801 ggaggatctg gacgtctccg gggcggtcga tccgttcgac cggaagcgca gaccgaacct
     4861 ggcccggtgg ctagcgttcg aggagcaacc gtttgacgtg atcgtggcgt accgggtaga
     4921 tcggttgacc cgatcgatcc ggcatcttca gcagctggtc cactgggccg aggaccacaa
     4981 gaagctggtc gtctccgcga ccgaagcgca cttcgatacg acgacgccgt ttgcggcggt
     5041 cgtcatcgcg cttatgggaa cggtggcgca gatggaatta gaagcgatca aagagcggaa
     5101 ccgttcggct gcgcatttca atatccgcgc cgggaaatac cgaggatccc tgccgccgtg
     5161 gggatacctg cctacgcgcg tggacgggga gtggcggctg gtgccggacc ctgtgcagcg
     5221 agagcgcatc ctcgaggtgt atcaccgcgt cgtcgacaac cacgagccgc tgcatctggt
     5281 ggcccacgac ctgaaccggc gtggtgtcct gtcgccgaag gactacttcg cgcagctgca
     5341 aggccgcgag ccgcagggcc gggagtggtc ggctaccgcg ctgaagcgat cgatgatctc
     5401 cgaggcgatg ctcgggtacg cgactctgaa cggtaagacc gtccgagacg acgacggagc
     5461 cccgctggtg cgggctgagc cgatcctgac ccgtgagcag ctggaggcgc tgcgcgccga
     5521 gctcgtgaag acctcccggg cgaagcccgc ggtgtctacc ccgtcgctgc tgctgcgggt
     5581 gttgttctgc gcggtgtgcg gggagcccgc gtacaagttc gccgggggag gacgtaagca
     5641 cccgcgctac cgctgccgct cgatggggtt cccgaagcac tgcgggaacg gcacggtggc
     5701 gatggccgag tgggacgcgt tctgcgagga gcaggtactg gatctgctcg gggacgcgga
     5761 gcgtctggag aaagtctggg tagcgggctc ggactccgcg gtcgaactcg cggaggtgaa
     5821 cgcggagctg gtggacctga cgtcgctgat cggctccccg gcctaccggg cgggctctcc
     5881 gcagcgagaa gcactggatg cccgtattgc ggcgctggcc gcgcggcaag aggagctgga
     5941 gggcctggag gctcgcccgt ctggctggga gtggcgcgag accgggcagc ggttcgggga
     6001 ctggtggcgg gagcaggaca ccgcggcaaa gaacacctgg cttcggtcga tgaacgttcg
     6061 gctgacgttc gacgtccgcg gcgggctgac tcgcacgatc gacttcgggg atcttcagga
     6121 gtacgagcag catctcaggc tcggcagcgt ggtcgaacgg ctacacaccg ggatgtcggg
     6181 ttcagcagca aacgacgaaa actacgctgc acaccgatga tgcttaagtc gaacagaaag
     6241 taatcgtatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca
     6301 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     6361 agagggcggc ccaactggca attccgacgt ctgtgcaagt actactgttc tgcagtcact
     6421 tgcctaagat actagctccg gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc
     6481 tttaaaaccg aaaagattac ttcgcgtttg ccacctgacg tctaagaaaa ggaatattca
     6541 gcaatttgcc cgtgccgaag aaaggcccac ccgtgaaggt gagccagtga gttgattgct
     6601 acgtaattag ttagttagcc cttagtgact cgaatttcgg ccggcttgtc gacgacggcg
     6661 gtctccgtcg tcaggatcat ccgggcaagc tttccacaca ttataggtac aaaaagatgc
     6721 gaaagcaaat aaattttttg ggtttgtacc gtacaccact gagaccgcgg tggttgacca
     6781 gacaaaccac gaagctgtca ccggatgtgc tttccggtct gatgagtccg tgaggacgaa
     6841 acagcctcta caaataattt tgtttaaggg cccaagttca cgagaacgat atagaaagtt
     6901 cttcattgtg gtggatttat taacattaat agagcgctga tgcctatagg ttggcgcatg
     6961 agttgcattc tacatgggcg tgcagtaaca agtctgtacg tagaacctaa ttacatatgt
     7021 aatttattaa cattttctag gagacgccat catggctaaa gtacaaaaat caacagactc
     7081 ttccagcttg gctgaagtag tagaccgtat tctggacaaa ggcattgtga ttgacgcttg
     7141 ggtcaaagtc tcattggttg gtattgaact gttatccatc gaagcacgtg ttgttattgc
     7201 ttctgttgaa acttatttga aatacgcaga agcgattggc cttacagcca gtgcagcgac
     7261 tccagcgtaa ttaagtaagc agaataaaag tgcccaagtg gctgagtcat tcagctgctt
     7321 gagtactttt caacgcttca agagtgctct atcacataag gagaatccta tgggatgctt
     7381 aacagacggt atggcgcagc tgcgtaagaa tattgatgac agtcatgaat ctcgcatagc
     7441 acagcaaaat gcgcgggttt ccagtgtgag tgcgcagatt gccggttttt ctacaactcg
     7501 ggctagaaac gcggctcaag acgcacgcgc acgcgcgaca ttcgttgccg ataatgtcag
     7561 aggcgttaat cggatgttga gtgacttctg tcatacccgc gaggtaatga gtcgtcaaca
     7621 gagtgaagaa cgtgccacct tcgtgacgga tatgtcgaag aaaacgctag ctttattaga
     7681 cggttttaat gctgagcgta aaagtatggc tgaacgctgt gctaaagaac gagctgattt
     7741 cattgccaac gtggcaaacg atgtcgctgc ttttcttagt gcttctgaga aggacagaat
     7801 ggctgcccat gctgtatttt tcggcatgac cctggctaaa aaaaaaacga gcctcgcagt
     7861 ctagccactc gtcctcttga taatactctg gcggtaagta gtgaagatga aaaaaggtaa
     7921 gtagcggtat tagcctctgt cgtccatgga gggtgaggtg gatgaacagt ctgctcacaa
     7981 acacggagtg acattattgg gcatacagtt tcgattaatc gctaagttaa atcgaaatat
     8041 aagaatccgc taaaatcgac cgattctagc ggctaagacg atcactgatt tggccatttc
     8101 gtagaactgg tgttggctag ggcgtacacg gtttggcaca ggagtatttc catgattaag
     8161 caaaataccg tttcgcagta taccgtagac gatgatcttg tcgttccgga agcgagtgaa
     8221 cattttgtag cgacttctta tgtgaacgac attatagaac gcgcattggt ttatttacgc
     8281 gctggctatc ccgtgcattt tgccggccca tctgggatag ggaaaacaac cctggcattt
     8341 catctcgcag cactatgggg tcgtcccgtt accatgttgc aaggtaacga agaatttgtg
     8401 agctccgatt tgacggggaa agacatcggc tatcgtaaaa gcagcctggt ggataactac
     8461 attcattcag tgcttaaaac tgaagaacag atgaaccgca tgtgggtgga taaccgtttg
     8521 accaccgcgt gtcgtaacgg cgacatgctg atctatgatg agttcaatcg ttccaaagca
     8581 gaaaccaata atgtgctgtt atccgtatta tccgaaggga ttttaaacct tcctggttta
     8641 cgtggtgtag gggaagggta tctcgatgta cacccagaat ttcgcgctat ttttacctcc
     8701 aatccagaag agtatgccgg tacgcataag acccaggacg ctctgatgga tcgtatgatt
     8761 actatcaaca ttggtcttgt cgatcgggat actgaactac agatactgca tgcgcgctct
     8821 gaattggaat taaaagaagc ggcatatatc gttgatataa tcagagagtt gcgtggtaac
     8881 gaacacgaaa ccaaacatgg gttacgtgca ggaattgcca tcgcccacat cctacaccag
     8941 caaggaataa aaccacgtta tggtgacaaa ttattccatg ctatctgcta cgacgtcttg
     9001 agtatggacg ctgccaaaat acaacacgct ggacgttcta tataccgtga aatggtcgat
     9061 ggcgttattc gtaaaatctg tccaccaata ggttcagaca cggttaaagc atccacacaa
     9121 aaaataaaag cagtggagta attatcatgg ctataagtac tcgtcctctc agaacactgt
     9181 cagacataaa aactcattca ggtcgagtga gtggtgagca tcaaacctat cgtgattatt
     9241 ttcagatagg tgcgctggaa ttagagcgtt ggcggcgaac acgagaacgt gaggctgcct
     9301 catcgcgtat tgccagtatt gatgagcgta tcgcggatat tgataaagaa aaagcagcgc
     9361 tgcttgcaga cgcgacagcg gcgagtgctg tggctgaaaa taatgataaa tcagaagcgg
     9421 ccgagaagaa gaaaaagtct tctggtttgc gaatcaagta ttgaggttgt cattatgaac
     9481 aaaattcgca ccctcgctca tatccgtaca ccgcgtatgg tgaagagctt tcgcacatgc
     9541 gtcaacatga cggggaagtc acaaattctc gctgattttg gacgtattta tttagaagaa
     9601 tatcgacgat tgaaccgtgt gcaatgcgcc aaaagtaaac gtatttcatt gaggagtgca
     9661 tgatgagtat tgataaatcc agaaatcatc gtgccaaggt gctttatgcg ctctgtgttt
     9721 ccgatgactc aacgcctaac tataaaatac gcggattgga agcagcaccg gtttattcta
     9781 ttgatcaaga tggcttaaga gctgtggtca gcgatacgct cagtacccgg ttacgtccag
     9841 aaagacgtaa tatcacggcg catcaggctg tattacataa gttgactgaa gaaggtaccg
     9901 tattgccgat gcgctttggg gttattgccc gtaatgctga agcagtaaag aatttgctgg
     9961 tcgctaatca ggatacgatc agagaacatt ttgaacgctt ggatggctgt gtggaaatgg
    10021 ggctacgtgt ttcctgggat gttactaata tttatgaata ttttgttgct acatatccag
    10081 tcttaagtga aacgcgcgat gaaatctgga atggaaattc caatgccaat aatcaccgtg
    10141 aagagaagat tcggctgggt aatttgtacg agtcattgcg tagtggagac agaaaagaat
    10201 cgacggaaaa agtaaaagaa gttctgttgg attattgcga agagattatt gaaaacccgg
    10261 ttaaaaaaga aaaagacgta atgaatctag cctgtttggt agcaagagaa cggatggatg
    10321 aatttgccaa aggcgttttt gaggcatcaa agttattcga taacgtttat ctgttcgatt
    10381 ataccggccc atgggcaccg cataatttcg ttactctgga tttacatgct cctacagcga
    10441 agaagaaaac gttgacccgc gctggtactc tctctgactg aggctaagcc atgctgctga
    10501 tcgatgacat cctgttttct cctgtcaaag gcgtcatgtg gatttttcgg caaattcacg
    10561 aactggcgga agatgaattg gctggtgagg cggatcgtat tcgtgaaagt ttgactgatc
    10621 tttatatgtt actggagact ggccagatta ccgaagatga atttgaacaa caggaagctg
    10681 tactgctgga tagattagac gcactggatg aagaagatga catgctaggc gacgagcctg
    10741 gggatgacga agacgacgaa tacgaagagg acgacgacga agaggacgac gacgaagagg
    10801 atgacgacga cgaggatgac gacgacgagg atgacgacga cgaagaggat gacgacgacg
    10861 acgaggatga cgacgacgag gatgagccag agggtacgac gaaatgaaac ctgctattta
    10921 tcccaagttc ttactggagt cgccgctgaa actggttttc ttcggcggta agggaggagt
    10981 aggtaaaagt acctgtgcga cgtcgacggc gttaaggctg gcgcaggaac agccgcagca
    11041 ccattttttg ctggtttcta ccgatccagc acattccctg caaaatattc tctccgatct
    11101 ggtattgcca aaaaatctgg acgtacgtga attaaatgcc gcagcctcct tgcatgaatt
    11161 taaatcgcag catgaaggtg ttctgaaaga gatagcgtat cgcggcactg ttctcgatca
    11221 aaacgacgta caaggattga tggataccgc gcttcccggc atggatgaat tggccgctta
    11281 tcttgaaata gctgaatgga ttcaaaaaga tacttactat cgtattatta ttgatactgc
    11341 tcctacaggt catacattgc ggcttttgga aatgccggat ttgatttatc ggtggttgac
    11401 cgctctggat accctgcttg ccaaacagcg ttatatccgg aaacgttttg ccggtgataa
    11461 tcgattggat catctggatc attttctgct ggacatgaat gattcactta aagctatgca
    11521 cgagctggtg acggattcca cgcgttgttg ttttgtgtta gtgatgctgg cggaagcgat
    11581 gagtgtggaa gaaagcattg atcttgctgg cgcgttgaat cagcaacggg tgtttctgtc
    11641 cgatttagtg gtcaatcgac tatttcctga aaatgactgt ccaacctgtt gtgttgagcg
    11701 taacagacaa atgcttgcgc tccagaacgg ataccaacgt ttgcctgggc acgtattctg
    11761 gacgttgcct ttgcttgcca tcgaaccgcg tggtgcgttg cttcacgaat tttggtccgg
    11821 ggtccgttta cttgatgaaa acgaagtcat ggctacgacg tgtcatcatc aactgccttt
    11881 acgtgtagag agttccattt cgctgcctgc cagcacattc cgactgctga tttttgcagg
    11941 taaaggtggg gtaggtaaaa cgacgctggc ctgtgctacg gcattgcgtc tgaacagtga
    12001 atatccagaa ttgcgtattc tgttgttctc agccgatcct gctcactcgc tgagtgattg
    12061 tctgggtgta accctacagc agcagcctat atcggtgttg gttaacattg atgcccagga
    12121 aattaatgca caagctgatt ttgacaaaat ccggcaggga tatcgtgcgg aattagaagc
    12181 tttcttgctg gatacgttac ccaatctgga tattactttt gatcgtgaag tgttggagca
    12241 tttgctcgat ctcgcgcccc ccggattaga tgaaatcatg gcattgactg cgatcatgga
    12301 tcatctagac agcggtcgtt atgacatggt catcgtggat ggcgcaccta gcggacattt
    12361 gctacgttta ttagagttgc ctgaattgat tcgggattgg ctaaaacagt ttttctcgtt
    12421 attgctgaaa tatcgcaaag tcatgcgttt cccgcattta tcggaacgtt tggtacagct
    12481 ttcacgcgaa ttaaaaaatc tacgcgctct actgcaggat accaaacaaa cggggttgta
    12541 tgcagttact gttccgacgc atttggcact tgagaagacc tatgaaatga catgcgcatt
    12601 gcagcgatta ggcttaacgg caaacgcgtt gtttatcaac cagattacac ctcccagcga
    12661 ttgcacactg tgccaggcaa tcacgagtcg agagtcgttg gagctgaagt gcgcagatga
    12721 gatgttccac agccaaccac acgcgcagat tttccggcaa acagaaccta cgggattgag
    12781 caagctaaag acattgggtt cggcattgtt tttataaact gtgagcgtac acattatgcc
    12841 agtgaacaaa cagtatcagg atgaacagca acaggtctct ctgtgtgagg ccttggaccg
    12901 cgttctgaac aaaggtgtgg tgattgtcgc tgatatcacc atttcagtcg ctaatattga
    12961 tttgatttat ttaagcctac aggctttggt ttcttctgta gaagcgaaaa accgtttacc
    13021 cggtagggag taacactatg acaaccaatc agttatcgca ccatagtcct gtgttcggcc
    13081 cgacatcgcc agcaatacaa cggccaatta ctgaagctaa tcgacataaa atagacatcg
    13141 atggagaacg cgtccgtgat ggtttggcgc aactcgtcct gaccctggtc aaactgctgc
    13201 acgaactact ggagcgtcaa gccataaggc ggatggattc gggttcgctt agtgatgaag
    13261 aagtcgagcg tttgggtttg gcgctaatgc gccaagccga ggagttgaca catttgtgcg
    13321 acgtttttgg cttcaaggat gatgatttga acttggattt aggcccgcta ggccgattgc
    13381 tgtaaggaaa ttgtatgatg aacaacaaca tacgattatt ttttagttcc catataaatg
    13441 ttatcagtct gttttcggca gagggtgtgt accatggtta atactaccaa tgacataaat
    13501 gcggctacgc gtggtcttct actgcgtatg ggtaatgcat ggtttgaaca agatgaactg
    13561 cgacaagcag tggatatcta tctgaaaata atcgagcaat atcctgacag caaggagagt
    13621 aaaaccgcgc aaacggcgct gttgaccatt tcacaacgtt atgaacgaga tggtttgttc
    13681 cggctgtcac ttgatatcct tgaacgcgtt ggggaaataa caccgacatc catataagtt
    13741 tcaggatatt aacgtggcct atttatcagg tttgaatcct atattaagtc gcaagcgata
    13801 tcgccagcag tgttttctac gcagtgagat ccatgttcag gataccctgg atccaagaat
    13861 taagagcatc agtacggtat agatcatctt tcaagttgca ggtgcgttgt ctgcccacgc
    13921 taaccccagt cacttactgg agtaagcctt ggggatttac tcgattgtcg ccttcctgtg
    13981 tgttgaaatt tattgggtat atatgctttt gattttagag agaaggggat gaggatttga
    14041 ggtctttagg ttgtttaccc tataaggatt tgcaccatga gcggcaacaa aaaactaacc
    14101 cattcaaccg attcgaccac cgtagcggat ttgttagagc gattactgga caaaggggtt
    14161 gtcatcagcg gtgacattcg catcagactg gttgaagtcg aattgctgac actggaaatt
    14221 cgcctgctta tctgttccgt agataaagcg gtagaaatgg ggctggattg gtggagtggc
    14281 aatccggcgt ttgattccag agccagagtg tcatcatcag cgcctgctcc agagttggaa
    14341 gaacgtctgc agcggctaga ggctcgtttg gaagcggcac cgtccgtcat agaagaaact
    14401 catttataag gattatcttt tttatgcgag cgctgattca ttttcccatt attcatagcc
    14461 ctaaagattt agggacattg agcgaagcgg caagtcattt gcgaaccgaa actcaaacgc
    14521 gtgcgtatct tgctgccgtc gaggggttct ggacaatgat taccactacg attgagggtt
    14581 tagatctgga ctatacacat ctgaaacttt atcaggatgg tctgccggtt tgtggaaaag
    14641 aaaatgaaat agtgaccgat gttgctaacg ctggtagcca gaattacaag ctcttgctta
    14701 cgttgcaaca taaaggcgca attctgatgg gaacagagtc ccctgaacta ttgttgcaag
    14761 agcgcgatct gatgacgcag ttgttacaat ctacggagca aaccgaagcg tccttggaaa
    14821 cagcaaaaac attattgaac cggcgggatg attatattgc ccagcgcatt gatgaaacgc
    14881 tgcaagatgg cgagatggcg atacttttcc tggggcttat gcacaatatt gaagcgaagc
    14941 tgcccgcaga tattgttttt attcaaccgt taggtaaacc cccaggaggg gaaagtattt
    15001 agtgactaca ttccttgttc tgcattatcg ttggcattag aagtggattt attcaccgag
    15061 aatattagag gtagtataaa tataatttat atgtcatttt aatagactac ataataatgg
    15121 gactttgttg ctttttttat atttttatgc gtttttttga ctgatgaaaa acgtaataat
    15181 aaccaactat gtttgtttgt aaaagtatcg gcatagcagc aatatagtgc gtatacggtg
    15241 ataaataaat attatggaac ccgtggtcgg aatttccatc acgggatgat tttgttaata
    15301 ctacccttcc tgtttgtctg tttcagcctt catttttgtg taaagaaatc gaaatcaata
    15361 ttcattattt tgttagataa tatatggtgc ttctagtgga attgatgcca gatgtgacat
    15421 aggcaaacgt tttttggcgg tggtttatta gctatttatg tgatgtatgt ctcttagtta
    15481 ttaccagttt tatcgatagc cctatgatct tttgatacta aaaatgtgac ctaatcggct
    15541 aaaaagggag tgtcgccctt gatatggctg atagccatca gtagacagat gatgggatga
    15601 tgtttatcat gtccagaata aagtctctcg cttatttgac cgggtagtta aaatattcgt
    15661 taataaatag aaatacttgc ctaaattatt gtcacaaaat cgaaaagaaa attgtgttat
    15721 cttaaatttg tactataagt attaaggatg ctattcatta aaattaggca ccgtctaggc
    15781 gatgattagg cggttgtgtt aaaaaaataa ctagagtgtt aataattctt tttttgcgtc
    15841 aaatatatac gtgatagaag ggagatttat cctcttcttc cccgtatctt ttaactatta
    15901 ttaaaaatga aataaccaat tggatgtagt cgatgaaaag tattgatcct ggctatgttt
    15961 taataattga tgacgacaaa gatatttgtg acctgctatc acacttgctg gaaaaggctg
    16021 gttgtgaaac tcagcaaggt tacgatggcg acatggcaat aaagttgttg tctcagcgtg
    16081 aaccggatgt actgttactt gatagtattc tccctaaaca cagtggcatg gaggtgttag
    16141 ccaatgccca tgctctgtat ccgcaactcc ccgttatcat tattactggc aatgtcggca
    16201 ttcttagcgc ggttggtgct atcaaagctg gtgcatggga ttacattccc aagccgtttg
    16261 ataacaaacg agtattagat ctggtgaatc gtgcaatgca aatgcgttgg ggtaactacg
    16321 attcaaactg caaagccagc aaggatacta aaacgcgtat cgctgccatg atggggaata
    16381 gccgcgagat tcagtcggtg atcagagatg tcgttaacgt ggctcatacg gatttttctg
    16441 tcgttattca gggtgaaacg ggaactggca aagaattggt tgcgaaaaac atccatttag
    16501 ccagtcagca tgccacaggg gtttttgttc ccattgattg tggctccata cccgactcgt
    16561 tgatcgaaaa tgaactattt ggtcatgaaa aaggctcgta caccggggct gatcaggttc
    16621 atgcgggtaa gtttgaagcg gctgacggcg gtacgttatt cctggatgaa atcgccaaca
    16681 tgtctttatc cgcacaggcc aagctactgc gtgtgctaca agagcacgtt gtctatcgta
    16741 ttggcgcgac taaacccatt cctgtcaatg tgcgagtcct ggctgccagc aatgatgatc
    16801 tactggacgc ggtagttaaa gggaaatttc gcgaggatct ctattatcga ttgaatgagt
    16861 atgtcattcg gattccgccg ttacgcaatc ggcacgagga tattctgttt ttgtccaatc
    16921 ggtttatcgc cgaaaccagc gtcgaattga acaaaacacc gcctgatttg tccgttgcgg
    16981 ctaaagaaac actgttacgt tatccatggc cgggtaacgt gcgagagtta cgcgctgtca
    17041 tccgccgtgc tgttttggta tccgacaaaa gagtaggggt tgatgattta ggcctggtca
    17101 tcaaagaaga tcactttccg caatcaacga aaaatccgta tctgagaacg atacagaatt
    17161 tctgtctgga aggttcgtct cttaaagaga tcactcaact caatattgat caaatagagc
    17221 gaattatcat tcaagacaca ttagagaaaa caggcaataa caaggcagaa gcagcgcggc
    17281 gtttgaatat tgactataaa acgctgcact ctaaactcaa aaagatcaat gcagccgagg
    17341 taaacaatca tgagtagtaa aaaaaaaatg atgtgacagg gaatgttgaa ggaattttgc
    17401 gcggtttggg cgatctggtg gaaaaattgg ttgaaaccgg cgaacagata aagcgtagcg
    17461 gtgcgttcga tattgacacg aacgacggta agaatgccaa agccgtgtat ggtttctcca
    17521 tcaagatggg tttggatgga aaccaggaaa atcgggtaga accttttggc aacatacgcc
    17581 gggatgagca aaccggggaa gctacagtgc aggaagtgtc tgaaccgttg gttgatgtta
    17641 tcgaagaaag tgatcatgtg ctggtactgg cagaaatgcc gggggtcgct gatgaagatg
    17701 tgcaagtcga attgaatggc gacattctga cgttacactc ggaaagaggc agtaaaaaat
    17761 atcataaaga aatcgtgttg ccgtgctcat tcgatgacaa ggcgatggaa cggtcttgcc
    17821 gtaatggcat tttggaagtc aaattaggaa aatgaggcag tcatgtctga agaactcaag
    17881 ctcaaggtcg ccgaggctct gccaaaggat gctgggcgcg gttatgctcg cttagatccg
    17941 gccgatatgg ccaggttaaa tctggctgtc ggtgacatag tgcagttaac cagtaaaaaa
    18001 ggcaccggga ttgctaaatt gatgccgact tatcccgata tgcggaataa aggcatcgta
    18061 caactggatg gtttgacgcg gcgtaatacc agcctgagtt tagatgaaaa agtgcagatt
    18121 gaacccgctt cctgcaaaca cgccacacag atcgtcctca ttcccaccac gattacgccc
    18181 aaccaacgtg acctgtatta tatcggtagc ctgctcgatg ggttgcccgt acagaaaggc
    18241 gatctgctgc gcgcgcacct gtttggcagt cgttctgctg attttaaagt cgaaagcacc
    18301 atacctgatg gtgcggtact gattgatccg accacgacac tggtgattgg caaatcaaat
    18361 gctgtcggga attcgagtca tagcacacaa cgtttgtcgt atgaggatgt tggcggcctt
    18421 aaaaaccagg tgcggcgtat tcgagaaatg atcgaattac cattacgcta tcctgaggtc
    18481 tttgaacgtt taggtataga tgcccccaaa ggtgtactgc tctctggtcc cccgggatgt
    18541 ggtaaaaccc tcatcgcccg cattattgcg caagaaaccg atgcgcaatt ttttaccatc
    18601 agcggcccgg aaattgtgca taagttttat ggtgaaagtg aagcccactt acgcaaaatt
    18661 tttgaggaag ccggacgcaa agggccaagt atcatttttc tggatgaaat tgactctatt
    18721 gcaccacacc gcgacaaggt cgtcggtgat gtagaaaaac gtatcgttgc gcaactgctc
    18781 gcattaatgg atggtctcaa gaatcgaggc aaagtcattg tcattgccgc gaccaatcta
    18841 cctaatgcga ttgatcccgc gttgcgccgt ccgggacgct ttgaccgtga aatcagcatt
    18901 cctattccag accgtgaagg tcgccgcgag attatcgaaa ttcatagcac gggaatgccg
    18961 ctgaatgctg atgtcgatct gaacgtgctt gccgatatca ctcacggttt tgtcggtgcc
    19021 gatctggaag cgttatgccg tgaagccgcg atgagtgcgt tacgccgttt actgcctgag
    19081 atcgatttca gttcggcgga gctaccttat gatcgtctgg ccgaattaac ggtgatgatg
    19141 gatgattttc gtgccgcctt gtgtgaagta tcaccgtcag cgattcgtga gctgttcgtc
    19201 gatattcccg atgtgcgttg ggaagatgtc ggcggtttgg atgacgtacg ccgtcgattg
    19261 atcgaatccg tcgagtggcc tatcaagtac cctgagctct atgaacaagc gggtgttaag
    19321 ccgcctaaag gtctgctgtt agctgggcct cccggcgttg ggaaaacgct gatcgccaaa
    19381 gccgttgcta atgaaagtgg cgttaatgtc atttcagtta aaggtccggc gctgatgtcg
    19441 cgttatgtcg gtgattcgga aaaaggtgtc cgagagctgt ttctcaaagc tcgtcaggct
    19501 gcgccctgca ttattttcct tgatgaagtc gattctgtga ttcctgcccg taacgagggc
    19561 gctatcgact cacatgtcgc agaacgggtg ttgagccaat tcttatctga aatggatggg
    19621 ctggaagaac tcaaaggtgt tttcgttatg ggggcgacca atcgtgcgga cttgatcgat
    19681 cccgcgatgc tgcgaccagg acgttttgat gaaataatcg aattgggcct gcccgacgag
    19741 gacgcccgtc ggcagattct ggcggtgcat ttacgtaata aaccgctggg tgacaatatt
    19801 catgctgacg atttggcaga acgttgtgat ggtgccagcg gtgcagaatt ggccgcggtg
    19861 tgtaatcgtg ccgcattagc ggcattgcgt cgtgcaatcc agcagtctga agaggctgtt
    19921 ctgagcccgt caacggtagg agaaacccct gtggcgctga cggtacgcat tgaacagcat
    19981 gattttgctg aagtgattgc cgaaatgttt ggcgatgatg catgacgatg aacacagaag
    20041 cacagactga gcaggccatt tatctgtatg gcctgacgtt gcccgattta gccgcaccgc
    20101 caattttggg cgttgataat cagcatccga ttaatacaca tcaatgtgcc ggattgaatg
    20161 cggtgattag cccagtggca ctctccgatt tcacgggtga aaagggtgaa gacaatgtac
    20221 agaatgtcac ttggctaacg ccgcgtattt gtcggcatgc gcagattatt gacagcctga
    20281 tggcccaagg tccggtctat cctttgccct tcggcacgct gttttccagt cagaacgcgt
    20341 tggaacagga aatgaaaagc cgcgctactg acgtatttgt gtcgctacgt cgcataaccg
    20401 gctgtcagga atgggcactg gaagcgacac tggatcgtaa acaggccgtt gacgttttat
    20461 tcaccgaagg tctggactcc ggtcgctttt gcctacccga agccataggc cgccgccatt
    20521 tggaagagca gaaattacgc cgcaggttaa ccactgagtt gagtgactgg ttggcacatg
    20581 ctttgactgc catgcagaac gaattacatc ctttggtacg tgatttccgt tcacgtcggt
    20641 tgttggacga taaaattttg cattgggcgt atttactccc ggtggaggac gttgccgcgt
    20701 ttcaacagca ggttgctgac attgttgaac gctatgaagc ttacggtttt agttttcgcg
    20761 tgacaggccc gtgggcagcc tacagttttt gccaacctga cgaatcatga gcttactact
    20821 gtacggcatt gttgccgagg atactcaact ggcgcttgaa ccagacggtt cgccgcacgc
    20881 cggtgaagag ccgatgcagc ttgttaaagc ggcaacgttg gcggcgctgg tcaagccttg
    20941 tgaagcagac gtcagtcgcg agcccgcggc agcgttggct tttggccaac agataatgca
    21001 tgttcatcaa cagaccacga ttattccgat tcgttatggt tgtgtgttgg cagatgaaga
    21061 tgcggtaaca cagcatttac tcaaccatga ggcacattat caaacgcaat tggttgagct
    21121 ggaaaattgt gacgaaatgg gtattcggtt atccctggcg tcggctgagg ataatgccgt
    21181 tactacgccg caagcgagtg gtctggacta tttgcggagt cgaaaactcg cttatgccgt
    21241 acctgaacac gctgaacggc aggctgcact actgaataat gcgtttacgg ggttgtatcg
    21301 ccgacactgc gcggaaatca gtatgtttaa tggtcagcgg acttacctgt tgagctatct
    21361 ggtgccacga accgggttac aggcgtttcg cgaccaattt aatactctgg ccaacaacat
    21421 gacggatata ggggttatca gtgggccttg gcctccctat aacttcgcca gctaatttgt
    21481 taacgacagg ctaccatgtc agataatcct tttgaacagc agtcagaaaa ccactttgaa
    21541 cttctttaca acatgctatt ggatgcaatt ccttcttccg tgttaatggt ggatgagaaa
    21601 ctgcatgtgg tttcagtcaa tcagaatttt ttaatcaagg gtcatcgcgg caacccggat
    21661 acaattggtc gcccgttatc ggacgtcttt ccgagcgtca ttttggaaaa tacgggtctt
    21721 gaagcacata tccgggatgt attccgtagc ggattacccg ttaatggtca gaaaatgacc
    21781 taccgtgcgc caggcgtacc gattcggatt tattactatt ctattctacc catccccatt
    21841 aaccagatta tgctgttcat ggaggatgtc accgagcagg ttcgcctgtc tgaagaagtc
    21901 catcgagtag aacggcacct cgcttcagtt gtagaaaacg ccagcgacat tgttctttcg
    21961 accgatattg aaggatgcat attgacgtgg aattcagcgg cggaaaagct gtcaggttta
    22021 cccacgcatg aagtctatgg gcgatacttt tttgagttct gcgataccaa ccaaaaggag
    22081 gcggtgcgtc tgattttcat gcgtatgaag aacagcaatg aatcacatac gactgaattt
    22141 catttattaa cgcctaacaa tggtggcagc atattgattt cctgggtatt ttcaccaatg
    22201 aaggatgact atgggcaaac ggtgggcacc gtagttgtcg gtcgtgattt ggctgaacgt
    22261 cggaaattgg aactgcaatt acgtcaatca cagaagttga ccgcgttggg cgtcatggcg
    22321 ggcggtatcg ctcatgaaat tcgtaatcct cttgctgttt gttcttcagc ggcgcaattt
    22381 ttgctggaag acgagattac ggctgaattt cataaagagt gttcaagaaa aatacagact
    22441 aacattcaaa aagcctcggc catcattgag aatttactcc gttttgcccg ttcttccctc
    22501 gataccgaaa tggtagaggt caatctgatc acggtgctga aagaaactat cgacctgatc
    22561 gccaaccagg ccaaagtgca gaaaattcag atcctgttgc cgttgcaaaa aggaaatata
    22621 ttggtgtcag gtgtgtcggg cttactgcaa caggtgttca tgaatatttt tcttaatgcc
    22681 attaatgcga tgccggaagg cggtgtgtta agtattgcgg tagagacctt tgaagaccgg
    22741 gttattgtgc gtatttctga tactggcgta ggcattcccg ccggcgagtt ggataaaatt
    22801 ttcgatccgt tccacacgtc ttcacccgta ggccaaggta ctggtttggg tttgtccatt
    22861 tgttacagca ttatccaaca gcaccttggt tccatacagg cagagagttg tctgggtgaa
    22921 ggttccacta ttattgttac cttacctata ctctaaaatg atgaacgcac ccattctgat
    22981 agttgatgat gaacctgaca tgtgttgggc catttcccat attattcaat cacaaggcat
    23041 gagtgtcatt accgcttaca gtggggaaga tgcttgcgtt aagctgatga ccggcaattt
    23101 ttcctttgtg tttttagatg ccaagttacc ggatatggac ggtctggaag tggcgcgtcg
    23161 agccagtacc ttgctagagc gaagaccgcg tgtagtgctg gtttccggtt atcactatta
    23221 cgatgatccg gttattcgcc atgctatcgt tgaaggcatt atctgcggtt ttctggcgaa
    23281 gccttttacc aataaagagc tgctgaaaac attacagaca ctatcacagc cgagcgagta
    23341 gttgcgtcgc tagcttaatc aacgctctgc ttcttcgggg ggaattactg acacgctgca
    23401 ccttaaaacg ggcattatga cgatagtgct cgttttaagg tgcaaatggt gttttatcgt
    23461 tagcgtgatg tgatgagatg tgcagcttct tttttcgata catgagcgga gtcatcttca
    23521 tttcttttcg aaactgatta ataaaatagc ttgtactatt aaaccctact tgataggcga
    23581 cttccgtcac attgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
    23641 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
    23701 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
    23761 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
    23821 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
    23881 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
    23941 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
    24001 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    24061 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    24121 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    24181 tgaaagtaaa agatgctgaa gatcagttgg gtgcagcaaa ctattaactg gcgaactact
    24241 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    24301 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    24361 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    24421 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    24481 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    24541 ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    24601 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    24661 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    24721 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
    24781 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    24841 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
    24901 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    24961 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa aaggatctag
    25021 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    25081 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    25141 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    25201 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    25261 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    25321 acatacctcg ctctgctaat cctgttacca gtcaggcatt tgagaagcac acggtcacac
    25381 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac
    25441 cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt
    25501 attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac tgccttaaaa
    25561 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
    25621 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
    25681 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
    25741 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
    25801 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
    25861 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcacaccaga gcgatgaaaa
    25921 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
    25981 ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat
    26041 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
    26101 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
    26161 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
    26221 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
    26281 tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg
    26341 ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc
    26401 gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa
    26461 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
    26521 gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac
    26581 atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga
    26641 agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac
    26701 aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
    26761 gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac
    26821 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
    26881 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
    26941 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
    27001 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
    27061 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
    27121 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
    27181 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
    27241 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
    27301 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
    27361 agagcaagag attacgcgca gaccaaaacg atctcaa
//
