LOCUS       sequence-467801-       27414 bp    DNA     circular UNA 15-APR-2025
DEFINITION  Optimized thiosulfate sensor with recombinase switch and acoustic 
            reporter genes (bARGSer).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 27414)
  AUTHORS   Buss MT, Zhu L, Kwon JH, Tabor JJ, Shapiro MG
  TITLE     Probiotic acoustic biosensors for noninvasive imaging of gut 
            inflammation
  JOURNAL   bioRxiv 2024.09.23.614598
REFERENCE   2  (bases 1 to 27414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.09.23.614598"
COMMENT     Sequence Label: MB 93C1 thsS(t3)R-Bxb1_P7-bARGSer
FEATURES             Location/Qualifiers
     source          1..27414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1241..1275
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     terminator      3111..3182
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3198..3225
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     3292..3310
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3331..3365
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     terminator      4029..4100
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4116..4143
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             4696..6195
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      complement(6573..6602)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     protein_bind    6654..6703
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    6758..6805
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     misc_RNA        6812..6861
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             15871..15882
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(22509..22520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(23627..23644)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      23797..23883
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      23975..24002
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        24021..24112
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      24570..25025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(24657..24676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     24867..24888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(25581..26240)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWCEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     26155..26174
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(26241..26343)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(26735..26752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(26869..27414)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(26949..26968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcatc ttattaatca gataaaatag ggttatttcg atgaaaggag agagtactaa
       61 tgccaattgc aataggaaat aaacggttac ccgttacgtt ggatgaaaag agacaaaaag
      121 aattgcagca actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt
      181 tagatttatt gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac
      241 gtcacttttt atcctaaaaa ctaaaagtga tagcactttt aattataaga agttagaata
      301 ttaatcattt gcttaattgt acaatataat gtacaattgt tttatagaaa taaataaggg
      361 gtgaaaggaa tggaagcagt agcttattca aatttccgcc aaaatttacg tagttatatg
      421 aaacaagtta atgaggatgc tgaaacactt attgtaacaa gtaaagatgt agaagataca
      481 gttgttgtat tatcaaaaag agattatgat tctatgcaag aaacgttgag aacactttct
      541 aataattacg tcatggaaaa aattcgtcga ggagatgaac aattctccaa aggtgcattt
      601 aaaacacatg acttaatcga ggttgaatct gatgattaag gcttggtctg atgatgcttg
      661 ggatgattat ctttattggc atgagcaagg aaacaaaagc aatataaaaa agattaacaa
      721 gttaataaaa gatatcgatc gttccccctt tgctggatta ggaaaacctg agccattaaa
      781 gcatgattta tctggaaaat ggtccagaag aattacagat gaacatagac tgatatatag
      841 agttgaaaat gaaacgatat ttatttattc tgcaaaagat cactattaac caatcggaag
      901 taaggaaagg gtcagaaact taaaagtttt tgatccttat tttatttacc ctagtcattt
      961 aaaaagctaa tatagcttag tgttgattgt tattaatgaa tgtgtttgtt acgcgtatta
     1021 cggatataag gttagtaaaa tcatttctaa agttgaggaa aagtaaatat aaatggctta
     1081 aatttcaaca atttgaagtt gaatagatat gttataatac tattgtagtg tgggatgtta
     1141 gttactaaag gatgacgctt atatatatga ctgaatagaa taagcaatag gtttaataat
     1201 ctattttaaa ttttttgtac tagttttagt taattagcgg ttgacagcta gctcagtcct
     1261 aggtattgtg ctagcctagt atcgatctcc ataactatcc tatagatcat gtcccgcctg
     1321 ctgctgtgta tctgtgttct gctgttctct tctgtggcgt ggtctaaacc gcagcagttt
     1381 tatgtgggcg tactggctaa ctggggtcat cagcaagccg ttgaacgttg gaccccgatg
     1441 atggagtatc tgaacgaaca cgtgccggac gcggaatttc acgtctaccc gggcaacttc
     1501 aaagcactga acctggcaat ggaactgggc cagattcagt tcattatcac taacccgggc
     1561 caatatctgt acctgagcaa tcagtacccg ctgtcttggc tggcgaccat gcgttctaag
     1621 cgtcacgatg gtaccacttc tgcgatcggt tccgccatta ttgtccgcgc ggacagcgac
     1681 taccgcaccc tgtacgacct gaaaggtaaa gtggtggctg cgtccgaccc gcatgctctg
     1741 ggtggctacc aagcgaccgt cggtctgatg cattccctgg gcatggatcc ggacaccttc
     1801 ttcggtgaaa ccaagtttct gggctttcca ctggatccgc tgctgtacca agttcgtgat
     1861 ggcaacgttg acgcggccat taccccactg tgcactctgg aggacatggt tgcacgcggc
     1921 gtactgaaat cttccgattt tcgtgtgctg aaccctagcc gcccggatgg tgtagaatgc
     1981 cagtgctcta ccaccctgta cccgaactgg tctttcgctg cgactgagtc tgtatccacc
     2041 gaactgtcta aagaaatcac gcaggcactg ctggaactgc catccgacag cccggcagct
     2101 atcaaagcgc aactgaccgg ctggaccagc ccgatctccc aactggcggt aatcaaactg
     2161 ttccaagagc tgcacgtaaa aaccccggac tctagccgtt gggaagccgt taagaagtgg
     2221 ctggaagaaa accgtcactg gggtatcctg tctgttctgg tgttcatcat tgcaacgctg
     2281 tatcacctgt ggattgaata ccgcttccac caaaaaagct cctctctgat cgaatctgaa
     2341 cgtcagctga aacagcatgc tgttgccctg gaacgtctgc aatctgctag catcgttggt
     2401 gaaattggtg cgggtctggc ccacgagatt aatcagccga tcgctgcaat tacctcttat
     2461 tctgaaggtg gcatcatgcg cctgcaaggt aaagaacagg cggatacgga tagctgcatc
     2521 gaactgctgg aaaaaatcca caaacagagc actcgcgcag gcgaagtggt gcaccgcatc
     2581 cgtggtctgc tgaaacgtcg tgaagcggtg atggtagatg ttaacatcct gaccctggtg
     2641 gaagaatcca tcagcctgct gcgtctggag ctggcacgtc gcgaaatcca gatcaacact
     2701 cagatcaaag gtgaaccgtt cttcattact gccgaccgcg ttggcctgct gcaagttctg
     2761 attaacctga tcaaaaactc cctggacgcg atcgctgaat ctgataatgc ccgttctggt
     2821 aaaatcaaca tcgaactgga ctttaaagag taccaggtaa acgtctccat catcgataac
     2881 ggtccgggcc tggcgatgga ttctgacact ctgatggcta cgttttacac taccaaaatg
     2941 gatggcctgg gcctgggtct ggcagtctgc cgcgaagtta tcagcaacca cgacggccac
     3001 atcctgctgt ccaaccgtga cgacggcgtt ctgggctgtg tggcaaccct gaatctgaaa
     3061 aaacgcggtt ctgaagtgcc gatcgaagtc taataatcta gaccaggcat caaataaaac
     3121 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     3181 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat aggtacccgg
     3241 ggatcctcta gcgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3301 atcacgaggc cctttcgtct tcacctcgag tttacggcta gctcagtcct aggtactatg
     3361 ctagcagaaa tataaagaac gatctattta tccgcgtaca tgcagcagca aatcaacggc
     3421 ccggtctacc tggtggatga tgatgaagcc attatcgact ccatcgattt tttgatggag
     3481 ggctacggtt acaaactgaa ctcgtttaac tgcggcgatc gctttttggc agaagtcgat
     3541 ctcacccagg caggatgtgt aattctggat gcgcgtatgc caggcttaac tggtcctcag
     3601 gtgcaacagc tgctgagcga cgcgaaaagc ccgcttgcgg tcatcttcct gaccggccat
     3661 ggcgatgttc cgatggcggt tgatgcgttc aaaaatggcg cgttcgattt ctttcaaaaa
     3721 cctgtgccgg gtagcttgct cagtcagtca attgccaaag gcttgactta ttcaatcgat
     3781 caacatctga aacgtactaa ccaagcgtta atcgacacgc tctcggaacg cgaagctcaa
     3841 atttttcaac tggtgattgc aggcaacacc aacaaacaga tggctaacga gctttgcgtg
     3901 gctattcgta ccattgaggt tcaccgtagc aaactgatga ccaaactggg tgttaacaac
     3961 ctggctgaac tggttaaact ggcgccgctg ctggcacata aatccgaata ataatctaga
     4021 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     4081 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     4141 gcgtttatag taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
     4201 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctggttagc
     4261 tagtcattca agcattatta tgctgttttt tgaagtgaat gtgcggccat ctagccgcac
     4321 attttgcatc taaaacatgc agtcatcagc aaaataataa acttttcccc aatatgtggt
     4381 ttaccacaat ttacaggaat tcactcctgt ggtggtgcaa atttgaactg tgaattgctt
     4441 cacaaacgcc gctatcgcaa tgtcagtatg tggtttacca caatatctaa tatcactctg
     4501 ctcaataaca atgatgaaaa ccttaggaag aagttaattg tgttaaacag ttaactaggg
     4561 gctttatcta acgctctcct aaggacaact gtcattggga gatttaacac gcactgacca
     4621 ccatgaaggt gacgctcatg acttacttgc tgaatctcag gagtttatga cctttttttt
     4681 ttagatccta tcggtatgag agccctggta gtcatccgcc tgtcccgcgt caccgatgct
     4741 acgacttcac cggagcgtca gctggagtct tgccagcagc tctgcgccca gcgcggctgg
     4801 gacgtcgtcg gggtagcgga ggatctggac gtctccgggg cggtcgatcc gttcgaccgg
     4861 aagcgcagac cgaacctggc ccggtggcta gcgttcgagg agcaaccgtt tgacgtgatc
     4921 gtggcgtacc gggtagatcg gttgacccga tcgatccggc atcttcagca gctggtccac
     4981 tgggccgagg accacaagaa gctggtcgtc tccgcgaccg aagcgcactt cgatacgacg
     5041 acgccgtttg cggcggtcgt catcgcgctt atgggaacgg tggcgcagat ggaattagaa
     5101 gcgatcaaag agcggaaccg ttcggctgcg catttcaata tccgcgccgg gaaataccga
     5161 ggatccctgc cgccgtgggg atacctgcct acgcgcgtgg acggggagtg gcggctggtg
     5221 ccggaccctg tgcagcgaga gcgcatcctc gaggtgtatc accgcgtcgt cgacaaccac
     5281 gagccgctgc atctggtggc ccacgacctg aaccggcgtg gtgtcctgtc gccgaaggac
     5341 tacttcgcgc agctgcaagg ccgcgagccg cagggccggg agtggtcggc taccgcgctg
     5401 aagcgatcga tgatctccga ggcgatgctc gggtacgcga ctctgaacgg taagaccgtc
     5461 cgagacgacg acggagcccc gctggtgcgg gctgagccga tcctgacccg tgagcagctg
     5521 gaggcgctgc gcgccgagct cgtgaagacc tcccgggcga agcccgcggt gtctaccccg
     5581 tcgctgctgc tgcgggtgtt gttctgcgcg gtgtgcgggg agcccgcgta caagttcgcc
     5641 gggggaggac gtaagcaccc gcgctaccgc tgccgctcga tggggttccc gaagcactgc
     5701 gggaacggca cggtggcgat ggccgagtgg gacgcgttct gcgaggagca ggtactggat
     5761 ctgctcgggg acgcggagcg tctggagaaa gtctgggtag cgggctcgga ctccgcggtc
     5821 gaactcgcgg aggtgaacgc ggagctggtg gacctgacgt cgctgatcgg ctccccggcc
     5881 taccgggcgg gctctccgca gcgagaagca ctggatgccc gtattgcggc gctggccgcg
     5941 cggcaagagg agctggaggg cctggaggct cgcccgtctg gctgggagtg gcgcgagacc
     6001 gggcagcggt tcggggactg gtggcgggag caggacaccg cggcaaagaa cacctggctt
     6061 cggtcgatga acgttcggct gacgttcgac gtccgcggcg ggctgactcg cacgatcgac
     6121 ttcggggatc ttcaggagta cgagcagcat ctcaggctcg gcagcgtggt cgaacggcta
     6181 cacaccggga tgtcgggttc agcagcaaac gacgaaaact acgctgcaca ccgatgatgc
     6241 ttaagtcgaa cagaaagtaa tcgtatgcga aacgatcctc atcctgtctc ttgatcagat
     6301 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     6361 ggcttcccaa ccttaccaga gggcggccca actggcaatt ccgacgtctg tgcaagtact
     6421 actgttctgc agtcacttgc ctaagatact agctccggca aaaaaacggg caaggtgtca
     6481 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgtttgcca cctgacgtct
     6541 aagaaaagga atattcagca atttgcccgt gccgaagaaa ggcccacccg tgaaggtgag
     6601 ccagtgagtt gattgctacg taattagtta gttagccctt agtgactcga atttcggccg
     6661 gcttgtcgac gacggcggtc tccgtcgtca ggatcatccg ggcaagcttt ccacacatta
     6721 taggtacaaa aagatgcgaa agcaaataaa ttttttgggt ttgtaccgta caccactgag
     6781 accgcggtgg ttgaccagac aaaccacgaa gctgtcaccg gatgtgcttt ccggtctgat
     6841 gagtccgtga ggacgaaaca gcctctacaa ataattttgt ttaagggccc aagttcacga
     6901 gaacgatata gaaagttctt cattgtggtg gatttattaa cattaataga gcgctgatgc
     6961 ctataggttg gcgcatgagt tgcattctac atgggcgtgc agtaacaagt ctgtacgtag
     7021 aacctaatta catatgtaat ttattaacat tttctaggag acgccatcat ggctaaagta
     7081 caaaaatcaa cagactcttc cagcttggct gaagtagtag accgtattct ggacaaaggc
     7141 attgtgattg acgcttgggt caaagtctca ttggttggta ttgaactgtt atccatcgaa
     7201 gcacgtgttg ttattgcttc tgttgaaact tatttgaaat acgcagaagc gattggcctt
     7261 acagccagtg cagcgactcc agcgtaatta agtaagcaga ataaaagtgc ccaagtggct
     7321 gagtcattca gctgcttgag tacttttcaa cgcttcaaga gtgctctatc acataaggag
     7381 aatcctatgg gatgcttaac agacggtatg gcgcagctgc gtaagaatat tgatgacagt
     7441 catgaatctc gcatagcaca gcaaaatgcg cgggtttcca gtgtgagtgc gcagattgcc
     7501 ggtttttcta caactcgggc tagaaacgcg gctcaagacg cacgcgcacg cgcgacattc
     7561 gttgccgata atgtcagagg cgttaatcgg atgttgagtg acttctgtca tacccgcgag
     7621 gtaatgagtc gtcaacagag tgaagaacgt gccaccttcg tgacggatat gtcgaagaaa
     7681 acgctagctt tattagacgg ttttaatgct gagcgtaaaa gtatggctga acgctgtgct
     7741 aaagaacgag ctgatttcat tgccaacgtg gcaaacgatg tcgctgcttt tcttagtgct
     7801 tctgagaagg acagaatggc tgcccatgct gtatttttcg gcatgaccct ggctaaaaaa
     7861 aaaacgagcc tcgcagtcta gccactcgtc ctcttgataa tactctggcg gtaagtagtg
     7921 aagatgaaaa aaggtaagta gcggtattag cctctgtcgt ccatggaggg tgaggtggat
     7981 gaacagtctg ctcacaaaca cggagtgaca ttattgggca tacagtttcg attaatcgct
     8041 aagttaaatc gaaatataag aatccgctaa aatcgaccga ttctagcggc taagacgatc
     8101 actgatttgg ccatttcgta gaactggtgt tggctagggc gtacacggtt tggcacagga
     8161 gtatttccat gattaagcaa aataccgttt cgcagtatac cgtagacgat gatcttgtcg
     8221 ttccggaagc gagtgaacat tttgtagcga cttcttatgt gaacgacatt atagaacgcg
     8281 cattggttta tttacgcgct ggctatcccg tgcattttgc cggcccatct gggataggga
     8341 aaacaaccct ggcatttcat ctcgcagcac tatggggtcg tcccgttacc atgttgcaag
     8401 gtaacgaaga atttgtgagc tccgatttga cggggaaaga catcggctat cgtaaaagca
     8461 gcctggtgga taactacatt cattcagtgc ttaaaactga agaacagatg aaccgcatgt
     8521 gggtggataa ccgtttgacc accgcgtgtc gtaacggcga catgctgatc tatgatgagt
     8581 tcaatcgttc caaagcagaa accaataatg tgctgttatc cgtattatcc gaagggattt
     8641 taaaccttcc tggtttacgt ggtgtagggg aagggtatct cgatgtacac ccagaatttc
     8701 gcgctatttt tacctccaat ccagaagagt atgccggtac gcataagacc caggacgctc
     8761 tgatggatcg tatgattact atcaacattg gtcttgtcga tcgggatact gaactacaga
     8821 tactgcatgc gcgctctgaa ttggaattaa aagaagcggc atatatcgtt gatataatca
     8881 gagagttgcg tggtaacgaa cacgaaacca aacatgggtt acgtgcagga attgccatcg
     8941 cccacatcct acaccagcaa ggaataaaac cacgttatgg tgacaaatta ttccatgcta
     9001 tctgctacga cgtcttgagt atggacgctg ccaaaataca acacgctgga cgttctatat
     9061 accgtgaaat ggtcgatggc gttattcgta aaatctgtcc accaataggt tcagacacgg
     9121 ttaaagcatc cacacaaaaa ataaaagcag tggagtaatt atcatggcta taagtactcg
     9181 tcctctcaga acactgtcag acataaaaac tcattcaggt cgagtgagtg gtgagcatca
     9241 aacctatcgt gattattttc agataggtgc gctggaatta gagcgttggc ggcgaacacg
     9301 agaacgtgag gctgcctcat cgcgtattgc cagtattgat gagcgtatcg cggatattga
     9361 taaagaaaaa gcagcgctgc ttgcagacgc gacagcggcg agtgctgtgg ctgaaaataa
     9421 tgataaatca gaagcggccg agaagaagaa aaagtcttct ggtttgcgaa tcaagtattg
     9481 aggttgtcat tatgaacaaa attcgcaccc tcgctcatat ccgtacaccg cgtatggtga
     9541 agagctttcg cacatgcgtc aacatgacgg ggaagtcaca aattctcgct gattttggac
     9601 gtatttattt agaagaatat cgacgattga accgtgtgca atgcgccaaa agtaaacgta
     9661 tttcattgag gagtgcatga tgagtattga taaatccaga aatcatcgtg ccaaggtgct
     9721 ttatgcgctc tgtgtttccg atgactcaac gcctaactat aaaatacgcg gattggaagc
     9781 agcaccggtt tattctattg atcaagatgg cttaagagct gtggtcagcg atacgctcag
     9841 tacccggtta cgtccagaaa gacgtaatat cacggcgcat caggctgtat tacataagtt
     9901 gactgaagaa ggtaccgtat tgccgatgcg ctttggggtt attgcccgta atgctgaagc
     9961 agtaaagaat ttgctggtcg ctaatcagga tacgatcaga gaacattttg aacgcttgga
    10021 tggctgtgtg gaaatggggc tacgtgtttc ctgggatgtt actaatattt atgaatattt
    10081 tgttgctaca tatccagtct taagtgaaac gcgcgatgaa atctggaatg gaaattccaa
    10141 tgccaataat caccgtgaag agaagattcg gctgggtaat ttgtacgagt cattgcgtag
    10201 tggagacaga aaagaatcga cggaaaaagt aaaagaagtt ctgttggatt attgcgaaga
    10261 gattattgaa aacccggtta aaaaagaaaa agacgtaatg aatctagcct gtttggtagc
    10321 aagagaacgg atggatgaat ttgccaaagg cgtttttgag gcatcaaagt tattcgataa
    10381 cgtttatctg ttcgattata ccggcccatg ggcaccgcat aatttcgtta ctctggattt
    10441 acatgctcct acagcgaaga agaaaacgtt gacccgcgct ggtactctct ctgactgagg
    10501 ctaagccatg ctgctgatcg atgacatcct gttttctcct gtcaaaggcg tcatgtggat
    10561 ttttcggcaa attcacgaac tggcggaaga tgaattggct ggtgaggcgg atcgtattcg
    10621 tgaaagtttg actgatcttt atatgttact ggagactggc cagattaccg aagatgaatt
    10681 tgaacaacag gaagctgtac tgctggatag attagacgca ctggatgaag aagatgacat
    10741 gctaggcgac gagcctgggg atgacgaaga cgacgaatac gaagaggacg acgacgaaga
    10801 ggacgacgac gaagaggatg acgacgacga ggatgacgac gacgaggatg acgacgacga
    10861 agaggatgac gacgacgacg aggatgacga cgacgaggat gagccagagg gtacgacgaa
    10921 atgaaacctg ctatttatcc caagttctta ctggagtcgc cgctgaaact ggttttcttc
    10981 ggcggtaagg gaggagtagg taaaagtacc tgtgcgacgt cgacggcgtt aaggctggcg
    11041 caggaacagc cgcagcacca ttttttgctg gtttctaccg atccagcaca ttccctgcaa
    11101 aatattctct ccgatctggt attgccaaaa aatctggacg tacgtgaatt aaatgccgca
    11161 gcctccttgc atgaatttaa atcgcagcat gaaggtgttc tgaaagagat agcgtatcgc
    11221 ggcactgttc tcgatcaaaa cgacgtacaa ggattgatgg ataccgcgct tcccggcatg
    11281 gatgaattgg ccgcttatct tgaaatagct gaatggattc aaaaagatac ttactatcgt
    11341 attattattg atactgctcc tacaggtcat acattgcggc ttttggaaat gccggatttg
    11401 atttatcggt ggttgaccgc tctggatacc ctgcttgcca aacagcgtta tatccggaaa
    11461 cgttttgccg gtgataatcg attggatcat ctggatcatt ttctgctgga catgaatgat
    11521 tcacttaaag ctatgcacga gctggtgacg gattccacgc gttgttgttt tgtgttagtg
    11581 atgctggcgg aagcgatgag tgtggaagaa agcattgatc ttgctggcgc gttgaatcag
    11641 caacgggtgt ttctgtccga tttagtggtc aatcgactat ttcctgaaaa tgactgtcca
    11701 acctgttgtg ttgagcgtaa cagacaaatg cttgcgctcc agaacggata ccaacgtttg
    11761 cctgggcacg tattctggac gttgcctttg cttgccatcg aaccgcgtgg tgcgttgctt
    11821 cacgaatttt ggtccggggt ccgtttactt gatgaaaacg aagtcatggc tacgacgtgt
    11881 catcatcaac tgcctttacg tgtagagagt tccatttcgc tgcctgccag cacattccga
    11941 ctgctgattt ttgcaggtaa aggtggggta ggtaaaacga cgctggcctg tgctacggca
    12001 ttgcgtctga acagtgaata tccagaattg cgtattctgt tgttctcagc cgatcctgct
    12061 cactcgctga gtgattgtct gggtgtaacc ctacagcagc agcctatatc ggtgttggtt
    12121 aacattgatg cccaggaaat taatgcacaa gctgattttg acaaaatccg gcagggatat
    12181 cgtgcggaat tagaagcttt cttgctggat acgttaccca atctggatat tacttttgat
    12241 cgtgaagtgt tggagcattt gctcgatctc gcgccccccg gattagatga aatcatggca
    12301 ttgactgcga tcatggatca tctagacagc ggtcgttatg acatggtcat cgtggatggc
    12361 gcacctagcg gacatttgct acgtttatta gagttgcctg aattgattcg ggattggcta
    12421 aaacagtttt tctcgttatt gctgaaatat cgcaaagtca tgcgtttccc gcatttatcg
    12481 gaacgtttgg tacagctttc acgcgaatta aaaaatctac gcgctctact gcaggatacc
    12541 aaacaaacgg ggttgtatgc agttactgtt ccgacgcatt tggcacttga gaagacctat
    12601 gaaatgacat gcgcattgca gcgattaggc ttaacggcaa acgcgttgtt tatcaaccag
    12661 attacacctc ccagcgattg cacactgtgc caggcaatca cgagtcgaga gtcgttggag
    12721 ctgaagtgcg cagatgagat gttccacagc caaccacacg cgcagatttt ccggcaaaca
    12781 gaacctacgg gattgagcaa gctaaagaca ttgggttcgg cattgttttt ataaactgtg
    12841 agcgtacaca ttatgccagt gaacaaacag tatcaggatg aacagcaaca ggtctctctg
    12901 tgtgaggcct tggaccgcgt tctgaacaaa ggtgtggtga ttgtcgctga tatcaccatt
    12961 tcagtcgcta atattgattt gatttattta agcctacagg ctttggtttc ttctgtagaa
    13021 gcgaaaaacc gtttacccgg tagggagtaa cactatgaca accaatcagt tatcgcacca
    13081 tagtcctgtg ttcggcccga catcgccagc aatacaacgg ccaattactg aagctaatcg
    13141 acataaaata gacatcgatg gagaacgcgt ccgtgatggt ttggcgcaac tcgtcctgac
    13201 cctggtcaaa ctgctgcacg aactactgga gcgtcaagcc ataaggcgga tggattcggg
    13261 ttcgcttagt gatgaagaag tcgagcgttt gggtttggcg ctaatgcgcc aagccgagga
    13321 gttgacacat ttgtgcgacg tttttggctt caaggatgat gatttgaact tggatttagg
    13381 cccgctaggc cgattgctgt aaggaaattg tatgatgaac aacaacatac gattattttt
    13441 tagttcccat ataaatgtta tcagtctgtt ttcggcagag ggtgtgtacc atggttaata
    13501 ctaccaatga cataaatgcg gctacgcgtg gtcttctact gcgtatgggt aatgcatggt
    13561 ttgaacaaga tgaactgcga caagcagtgg atatctatct gaaaataatc gagcaatatc
    13621 ctgacagcaa ggagagtaaa accgcgcaaa cggcgctgtt gaccatttca caacgttatg
    13681 aacgagatgg tttgttccgg ctgtcacttg atatccttga acgcgttggg gaaataacac
    13741 cgacatccat ataagtttca ggatattaac gtggcctatt tatcaggttt gaatcctata
    13801 ttaagtcgca agcgatatcg ccagcagtgt tttctacgca gtgagatcca tgttcaggat
    13861 accctggatc caagaattaa gagcatcagt acggtataga tcatctttca agttgcaggt
    13921 gcgttgtctg cccacgctaa ccccagtcac ttactggagt aagccttggg gatttactcg
    13981 attgtcgcct tcctgtgtgt tgaaatttat tgggtatata tgcttttgat tttagagaga
    14041 aggggatgag gatttgaggt ctttaggttg tttaccctat aaggatttgc accatgagcg
    14101 gcaacaaaaa actaacccat tcaaccgatt cgaccaccgt agcggatttg ttagagcgat
    14161 tactggacaa aggggttgtc atcagcggtg acattcgcat cagactggtt gaagtcgaat
    14221 tgctgacact ggaaattcgc ctgcttatct gttccgtaga taaagcggta gaaatggggc
    14281 tggattggtg gagtggcaat ccggcgtttg attccagagc cagagtgtca tcatcagcgc
    14341 ctgctccaga gttggaagaa cgtctgcagc ggctagaggc tcgtttggaa gcggcaccgt
    14401 ccgtcataga agaaactcat ttataaggat tatctttttt atgcgagcgc tgattcattt
    14461 tcccattatt catagcccta aagatttagg gacattgagc gaagcggcaa gtcatttgcg
    14521 aaccgaaact caaacgcgtg cgtatcttgc tgccgtcgag gggttctgga caatgattac
    14581 cactacgatt gagggtttag atctggacta tacacatctg aaactttatc aggatggtct
    14641 gccggtttgt ggaaaagaaa atgaaatagt gaccgatgtt gctaacgctg gtagccagaa
    14701 ttacaagctc ttgcttacgt tgcaacataa aggcgcaatt ctgatgggaa cagagtcccc
    14761 tgaactattg ttgcaagagc gcgatctgat gacgcagttg ttacaatcta cggagcaaac
    14821 cgaagcgtcc ttggaaacag caaaaacatt attgaaccgg cgggatgatt atattgccca
    14881 gcgcattgat gaaacgctgc aagatggcga gatggcgata cttttcctgg ggcttatgca
    14941 caatattgaa gcgaagctgc ccgcagatat tgtttttatt caaccgttag gtaaaccccc
    15001 aggaggggaa agtatttagt gactacattc cttgttctgc attatcgttg gcattagaag
    15061 tggatttatt caccgagaat attagaggta gtataaatat aatttatatg tcattttaat
    15121 agactacata ataatgggac tttgttgctt tttttatatt tttatgcgtt tttttgactg
    15181 atgaaaaacg taataataac caactatgtt tgtttgtaaa agtatcggca tagcagcaat
    15241 atagtgcgta tacggtgata aataaatatt atggaacccg tggtcggaat ttccatcacg
    15301 ggatgatttt gttaatacta cccttcctgt ttgtctgttt cagccttcat ttttgtgtaa
    15361 agaaatcgaa atcaatattc attattttgt tagataatat atggtgcttc tagtggaatt
    15421 gatgccagat gtgacatagg caaacgtttt ttggcggtgg tttattagct atttatgtga
    15481 tgtatgtctc ttagttatta ccagttttat cgatagccct atgatctttt gatactaaaa
    15541 atgtgaccta atcggctaaa aagggagtgt cgcccttgat atggctgata gccatcagta
    15601 gacagatgat gggatgatgt ttatcatgtc cagaataaag tctctcgctt atttgaccgg
    15661 gtagttaaaa tattcgttaa taaatagaaa tacttgccta aattattgtc acaaaatcga
    15721 aaagaaaatt gtgttatctt aaatttgtac tataagtatt aaggatgcta ttcattaaaa
    15781 ttaggcaccg tctaggcgat gattaggcgg ttgtgttaaa aaaataacta gagtgttaat
    15841 aattcttttt ttgcgtcaaa tatatacgtg atagaaggga gatttatcct cttcttcccc
    15901 gtatctttta actattatta aaaatgaaat aaccaattgg atgtagtcga tgaaaagtat
    15961 tgatcctggc tatgttttaa taattgatga cgacaaagat atttgtgacc tgctatcaca
    16021 cttgctggaa aaggctggtt gtgaaactca gcaaggttac gatggcgaca tggcaataaa
    16081 gttgttgtct cagcgtgaac cggatgtact gttacttgat agtattctcc ctaaacacag
    16141 tggcatggag gtgttagcca atgcccatgc tctgtatccg caactccccg ttatcattat
    16201 tactggcaat gtcggcattc ttagcgcggt tggtgctatc aaagctggtg catgggatta
    16261 cattcccaag ccgtttgata acaaacgagt attagatctg gtgaatcgtg caatgcaaat
    16321 gcgttggggt aactacgatt caaactgcaa agccagcaag gatactaaaa cgcgtatcgc
    16381 tgccatgatg gggaatagcc gcgagattca gtcggtgatc agagatgtcg ttaacgtggc
    16441 tcatacggat ttttctgtcg ttattcaggg tgaaacggga actggcaaag aattggttgc
    16501 gaaaaacatc catttagcca gtcagcatgc cacaggggtt tttgttccca ttgattgtgg
    16561 ctccataccc gactcgttga tcgaaaatga actatttggt catgaaaaag gctcgtacac
    16621 cggggctgat caggttcatg cgggtaagtt tgaagcggct gacggcggta cgttattcct
    16681 ggatgaaatc gccaacatgt ctttatccgc acaggccaag ctactgcgtg tgctacaaga
    16741 gcacgttgtc tatcgtattg gcgcgactaa acccattcct gtcaatgtgc gagtcctggc
    16801 tgccagcaat gatgatctac tggacgcggt agttaaaggg aaatttcgcg aggatctcta
    16861 ttatcgattg aatgagtatg tcattcggat tccgccgtta cgcaatcggc acgaggatat
    16921 tctgtttttg tccaatcggt ttatcgccga aaccagcgtc gaattgaaca aaacaccgcc
    16981 tgatttgtcc gttgcggcta aagaaacact gttacgttat ccatggccgg gtaacgtgcg
    17041 agagttacgc gctgtcatcc gccgtgctgt tttggtatcc gacaaaagag taggggttga
    17101 tgatttaggc ctggtcatca aagaagatca ctttccgcaa tcaacgaaaa atccgtatct
    17161 gagaacgata cagaatttct gtctggaagg ttcgtctctt aaagagatca ctcaactcaa
    17221 tattgatcaa atagagcgaa ttatcattca agacacatta gagaaaacag gcaataacaa
    17281 ggcagaagca gcgcggcgtt tgaatattga ctataaaacg ctgcactcta aactcaaaaa
    17341 gatcaatgca gccgaggtaa acaatcatga gtagtaaaaa aaaaatgatg tgacagggaa
    17401 tgttgaagga attttgcgcg gtttgggcga tctggtggaa aaattggttg aaaccggcga
    17461 acagataaag cgtagcggtg cgttcgatat tgacacgaac gacggtaaga atgccaaagc
    17521 cgtgtatggt ttctccatca agatgggttt ggatggaaac caggaaaatc gggtagaacc
    17581 ttttggcaac atacgccggg atgagcaaac cggggaagct acagtgcagg aagtgtctga
    17641 accgttggtt gatgttatcg aagaaagtga tcatgtgctg gtactggcag aaatgccggg
    17701 ggtcgctgat gaagatgtgc aagtcgaatt gaatggcgac attctgacgt tacactcgga
    17761 aagaggcagt aaaaaatatc ataaagaaat cgtgttgccg tgctcattcg atgacaaggc
    17821 gatggaacgg tcttgccgta atggcatttt ggaagtcaaa ttaggaaaat gaggcagtca
    17881 tgtctgaaga actcaagctc aaggtcgccg aggctctgcc aaaggatgct gggcgcggtt
    17941 atgctcgctt agatccggcc gatatggcca ggttaaatct ggctgtcggt gacatagtgc
    18001 agttaaccag taaaaaaggc accgggattg ctaaattgat gccgacttat cccgatatgc
    18061 ggaataaagg catcgtacaa ctggatggtt tgacgcggcg taataccagc ctgagtttag
    18121 atgaaaaagt gcagattgaa cccgcttcct gcaaacacgc cacacagatc gtcctcattc
    18181 ccaccacgat tacgcccaac caacgtgacc tgtattatat cggtagcctg ctcgatgggt
    18241 tgcccgtaca gaaaggcgat ctgctgcgcg cgcacctgtt tggcagtcgt tctgctgatt
    18301 ttaaagtcga aagcaccata cctgatggtg cggtactgat tgatccgacc acgacactgg
    18361 tgattggcaa atcaaatgct gtcgggaatt cgagtcatag cacacaacgt ttgtcgtatg
    18421 aggatgttgg cggccttaaa aaccaggtgc ggcgtattcg agaaatgatc gaattaccat
    18481 tacgctatcc tgaggtcttt gaacgtttag gtatagatgc ccccaaaggt gtactgctct
    18541 ctggtccccc gggatgtggt aaaaccctca tcgcccgcat tattgcgcaa gaaaccgatg
    18601 cgcaattttt taccatcagc ggcccggaaa ttgtgcataa gttttatggt gaaagtgaag
    18661 cccacttacg caaaattttt gaggaagccg gacgcaaagg gccaagtatc atttttctgg
    18721 atgaaattga ctctattgca ccacaccgcg acaaggtcgt cggtgatgta gaaaaacgta
    18781 tcgttgcgca actgctcgca ttaatggatg gtctcaagaa tcgaggcaaa gtcattgtca
    18841 ttgccgcgac caatctacct aatgcgattg atcccgcgtt gcgccgtccg ggacgctttg
    18901 accgtgaaat cagcattcct attccagacc gtgaaggtcg ccgcgagatt atcgaaattc
    18961 atagcacggg aatgccgctg aatgctgatg tcgatctgaa cgtgcttgcc gatatcactc
    19021 acggttttgt cggtgccgat ctggaagcgt tatgccgtga agccgcgatg agtgcgttac
    19081 gccgtttact gcctgagatc gatttcagtt cggcggagct accttatgat cgtctggccg
    19141 aattaacggt gatgatggat gattttcgtg ccgccttgtg tgaagtatca ccgtcagcga
    19201 ttcgtgagct gttcgtcgat attcccgatg tgcgttggga agatgtcggc ggtttggatg
    19261 acgtacgccg tcgattgatc gaatccgtcg agtggcctat caagtaccct gagctctatg
    19321 aacaagcggg tgttaagccg cctaaaggtc tgctgttagc tgggcctccc ggcgttggga
    19381 aaacgctgat cgccaaagcc gttgctaatg aaagtggcgt taatgtcatt tcagttaaag
    19441 gtccggcgct gatgtcgcgt tatgtcggtg attcggaaaa aggtgtccga gagctgtttc
    19501 tcaaagctcg tcaggctgcg ccctgcatta ttttccttga tgaagtcgat tctgtgattc
    19561 ctgcccgtaa cgagggcgct atcgactcac atgtcgcaga acgggtgttg agccaattct
    19621 tatctgaaat ggatgggctg gaagaactca aaggtgtttt cgttatgggg gcgaccaatc
    19681 gtgcggactt gatcgatccc gcgatgctgc gaccaggacg ttttgatgaa ataatcgaat
    19741 tgggcctgcc cgacgaggac gcccgtcggc agattctggc ggtgcattta cgtaataaac
    19801 cgctgggtga caatattcat gctgacgatt tggcagaacg ttgtgatggt gccagcggtg
    19861 cagaattggc cgcggtgtgt aatcgtgccg cattagcggc attgcgtcgt gcaatccagc
    19921 agtctgaaga ggctgttctg agcccgtcaa cggtaggaga aacccctgtg gcgctgacgg
    19981 tacgcattga acagcatgat tttgctgaag tgattgccga aatgtttggc gatgatgcat
    20041 gacgatgaac acagaagcac agactgagca ggccatttat ctgtatggcc tgacgttgcc
    20101 cgatttagcc gcaccgccaa ttttgggcgt tgataatcag catccgatta atacacatca
    20161 atgtgccgga ttgaatgcgg tgattagccc agtggcactc tccgatttca cgggtgaaaa
    20221 gggtgaagac aatgtacaga atgtcacttg gctaacgccg cgtatttgtc ggcatgcgca
    20281 gattattgac agcctgatgg cccaaggtcc ggtctatcct ttgcccttcg gcacgctgtt
    20341 ttccagtcag aacgcgttgg aacaggaaat gaaaagccgc gctactgacg tatttgtgtc
    20401 gctacgtcgc ataaccggct gtcaggaatg ggcactggaa gcgacactgg atcgtaaaca
    20461 ggccgttgac gttttattca ccgaaggtct ggactccggt cgcttttgcc tacccgaagc
    20521 cataggccgc cgccatttgg aagagcagaa attacgccgc aggttaacca ctgagttgag
    20581 tgactggttg gcacatgctt tgactgccat gcagaacgaa ttacatcctt tggtacgtga
    20641 tttccgttca cgtcggttgt tggacgataa aattttgcat tgggcgtatt tactcccggt
    20701 ggaggacgtt gccgcgtttc aacagcaggt tgctgacatt gttgaacgct atgaagctta
    20761 cggttttagt tttcgcgtga caggcccgtg ggcagcctac agtttttgcc aacctgacga
    20821 atcatgagct tactactgta cggcattgtt gccgaggata ctcaactggc gcttgaacca
    20881 gacggttcgc cgcacgccgg tgaagagccg atgcagcttg ttaaagcggc aacgttggcg
    20941 gcgctggtca agccttgtga agcagacgtc agtcgcgagc ccgcggcagc gtttgctttt
    21001 ggccaacaga taatgcatgt tcatcaacag accacgatta ttccgattcg ttatggttgt
    21061 gtgttggcag atgaagatgc ggtaacacag catttactca accatgaggc acattatcaa
    21121 acgcaattgg ttgagctgga aaattgtgac gaaatgggta ttcggttatc cctggcgtcg
    21181 gctgaggata atgccgttac tacgccgcaa gcgagtggtc tggactattt gcggagtcga
    21241 aaactcgctt atgccgtacc tgaacacgct gaacggcagg ctgcactact gaataatgcg
    21301 tttacggggt tgtatcgccg acactgcgcg gaaatcagta tgtttaatgg tcagcggact
    21361 tacctgttga gctatctggt gccacgaacc gggttacagg cgtttcgcga ccaatttaat
    21421 actctggcca acaacatgac ggatataggg gttatcagtg ggccttggcc tccctataac
    21481 ttcgccagct aatttgttaa cgacaggcta ccatgtcaga taatcctttt gaacagcagt
    21541 cagaaaacca ctttgaactt ctttacaaca tgctattgga tgcaattcct tcttccgtgt
    21601 taatggtgga tgagaaactg catgtggttt cagtcaatca gaatttttta atcaagggtc
    21661 atcgcggcaa cccggataca attggtcgcc cgttatcgga cgtctttccg agcgtcattt
    21721 tggaaaatac gggtcttgaa gcacatatcc gggatgtatt ccgtagcgga ttacccgtta
    21781 atggtcagaa aatgacctac cgtgcgccag gcgtaccgat tcggatttat tactattcta
    21841 ttctacccat ccccattaac cagattatgc tgttcatgga ggatgtcacc gagcaggttc
    21901 gcctgtctga agaagtccat cgagtagaac ggcacctcgc ttcagttgta gaaaacgcca
    21961 gcgacattgt tctttcgacc gatattgaag gatgcatatt gacgtggaat tcagcggcgg
    22021 aaaagctgtc aggtttaccc acgcatgaag tctatgggcg atactttttt gagttctgcg
    22081 ataccaacca aaaggaggcg gtgcgtctga ttttcatgcg tatgaagaac agcaatgaat
    22141 cacatacgac tgaatttcat ttattaacgc ctaacaatgg tggcagcata ttgatttcct
    22201 gggtattttc accaatgaag gatgactatg ggcaaacggt gggcaccgta gttgtcggtc
    22261 gtgatttggc tgaacgtcgg aaattggaac tgcaattacg tcaatcacag aagttgaccg
    22321 cgttgggcgt catggcgggc ggtatcgctc atgaaattcg taatcctctt gctgtttgtt
    22381 cttcagcggc gcaatttttg ctggaagacg agattacggc tgaatttcat aaagagtgtt
    22441 caagaaaaat acagactaac attcaaaaag cctcggccat cattgagaat ttactccgtt
    22501 ttgcccgttc ttccctcgat accgaaatgg tagaggtcaa tctgatcacg gtgctgaaag
    22561 aaactatcga cctgatcgcc aaccaggcca aagtgcagaa aattcagatc ctgttgccgt
    22621 tgcaaaaagg aaatatattg gtgtcaggtg tgtcgggctt actgcaacag gtgttcatga
    22681 atatttttct taatgccatt aatgcgatgc cggaaggcgg tgtgttaagt attgcggtag
    22741 agacctttga agaccgggtt attgtgcgta tttctgatac tggcgtaggc attcccgccg
    22801 gcgagttgga taaaattttc gatccgttcc acacgtcttc acccgtaggc caaggtactg
    22861 gtttgggttt gtccatttgt tacagcatta tccaacagca ccttggttcc atacaggcag
    22921 agagttgtct gggtgaaggt tccactatta ttgttacctt acctatactc taaaatgatg
    22981 aacgcaccca ttctgatagt tgatgatgaa cctgacatgt gttgggccat ttcccatatt
    23041 attcaatcac aaggcatgag tgtcattacc gcttacagtg gggaagatgc ttgcgttaag
    23101 ctgatgaccg gcaatttttc ctttgtgttt ttagatgcca agttaccgga tatggacggt
    23161 ctggaagtgg cgcgtcgagc cagtaccttg ctagagcgaa gaccgcgtgt agtgctggtt
    23221 tccggttatc actattacga tgatccggtt attcgccatg ctatcgttga aggcattatc
    23281 tgcggttttc tggcgaagcc ttttaccaat aaagagctgc tgaaaacatt acagacacta
    23341 tcacagccga gcgagtagtt gcgtcgctag cttaatcaac gctctgcttc ttcgggggga
    23401 attactgaca cgctgcacct taaaacgggc attatgacga tagtgctcgt tttaaggtgc
    23461 aaatggtgtt ttatcgttag cgtgatgtga tgagatgtgc agcttctttt ttcgatacat
    23521 gagcggagtc atcttcattt cttttcgaaa ctgattaata aaatagcttg tactattaaa
    23581 ccctacttga taggcgactt ccgtcacatt gagagaagat tttcagcctg atacagatta
    23641 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
    23701 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
    23761 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
    23821 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
    23881 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
    23941 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
    24001 tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct
    24061 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    24121 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    24181 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cagcaaacta
    24241 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    24301 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    24361 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    24421 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    24481 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    24541 gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc
    24601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    24661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    24721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    24781 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    24841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    24901 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg catattggtt
    24961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
    25021 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    25081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    25141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    25201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    25261 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
    25321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtc aggcatttga
    25381 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata
    25441 agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt
    25501 ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac
    25561 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
    25621 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
    25681 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
    25741 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
    25801 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
    25861 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
    25921 caccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
    25981 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc
    26041 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
    26101 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
    26161 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
    26221 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
    26281 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
    26341 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
    26401 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg
    26461 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
    26521 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
    26581 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact
    26641 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc
    26701 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
    26761 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
    26821 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
    26881 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
    26941 aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc
    27001 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
    27061 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
    27121 ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac
    27181 atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag
    27241 tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc
    27301 cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag
    27361 gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaa
//
