LOCUS       sequence-468014-        5781 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Localization: Membrane Adaptor, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5781)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Homer1-C-18
FEATURES             Location/Qualifiers
     source          1..5781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1227
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     polyA_signal    2509..2630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2546..2565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2600..2619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2637..3092)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2774..2795)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2986..3005
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3119..3223
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3225..3582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3225..3416
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3248..3268)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3433..3568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3617..4411
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3617..4411
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3671..3690)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4281..4300
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4599..4618)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4643..4690
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5019..5607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5508..5527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag tgtgattaaa ccagacatga agatcaagct gcgtatggaa
      601 ggcgctgtaa atggacaccc gttcgcgatt gaaggagttg gccttgggaa gcctttcgag
      661 ggaaaacaga gtatggacct taaagtcaaa gaaggcggac ctctgccttt cgcctatgac
      721 atcttgacaa ctgtgttctg ttacggcaac agggtattcg ccaaataccc agaaaatata
      781 gtagactatt tcaagcagtc gtttcctgag ggctactctt gggaacgaag catgaattac
      841 gaagacgggg gcatttgtaa cgcgacaaac gacataaccc tggatggtga ctgttatatc
      901 tatgaaattc gatttgatgg tgtgaacttt cctgccaatg gtccagttat gcagaagagg
      961 actgtgaaat gggagccatc cactgagaaa ttgtatgtgc gtgatggagt gctgaagggt
     1021 gatgttaaca tggctctgtc gcttgaagga ggtggccatt accgatgtga cttcaaaact
     1081 acttataaag ctaagaaggt tgtccagttg ccagactatc actttgtgga ccaccacatt
     1141 gagattaaaa gccacgacaa agattacagt aatgttaatc tgcatgagca cgccgaagcg
     1201 cattctgagc tgccgaggca ggccaagtcc ggagggagcg gcagtgggtc cagtggaggg
     1261 agcagtggaa gcggagggag cggggaacaa cctatcttca gcactcgagc tcatgtcttc
     1321 cagatcgacc caaacacaaa gaagaactgg gtacccacca gcaagcatgc agttactgtg
     1381 tcttatttct atgacagcac aaggaatgtg tataggataa tcagtttaga cggctcaaag
     1441 gcaataataa atagcaccat cactccaaac atgacattta ctaaaacatc tcaaaagttt
     1501 ggccaatggg ctgatagccg ggcaaacact gtttatggac tgggattctc ctctgagcat
     1561 catctctcaa aatttgcaga aaagtttcag gaatttaaag aagctgctcg gctggcaaag
     1621 gagaagtcgc aggagaagat ggaactgacc agtacccctt cacaggaatc agcaggagga
     1681 gatcttcagt ctcctttaac accagaaagt atcaatggga cagatgatga gagaacaccc
     1741 gatgtgacac agaactcaga gccaagggct gagccagctc agaatgcatt gccattttca
     1801 catagtgccg gggatcgaac ccagggcctc tctcatgcta gttcagccat cagcaaacac
     1861 tgggaggctg aactagccac gctcaagggg aacaatgcca agctcaccgc agcgctgctg
     1921 gagtccactg ccaacgtgaa gcagtggaag caacagctgg ctgcctacca ggaggaggca
     1981 gagcggctgc acaagcgggt cacggagctg gaatgtgtta gtagtcaagc aaacgcggtg
     2041 cacagccaca agacagagct gagtcagaca gtgcaggagc tggaagagac cctaaaagta
     2101 aaggaagagg aaatagaaag attaaaacaa gaaattgata acgccagaga acttcaagaa
     2161 cagagggact ctttgactca gaaactacag gaagttgaga ttcgaaataa agacctggag
     2221 gggcagctgt cggagctgga gcagcgcctg gagaagagcc agagcgagca ggacgctttc
     2281 cgcagtaacc tgaagactct cctagagatt ctggacggga aaatatttga actaacagaa
     2341 ttgcgggata atttggccaa gctactagaa tgcagctaag gatccaccgg atctagataa
     2401 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2461 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     2641 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2701 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2761 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2821 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2881 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2941 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3001 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3061 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3241 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3301 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3361 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3421 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3481 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3541 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3601 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3661 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3721 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3781 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3841 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3901 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3961 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4021 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4081 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4141 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4201 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4261 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4321 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4381 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4441 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4501 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4561 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4621 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4681 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4741 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4801 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     4861 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4921 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4981 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5041 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5101 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5161 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5221 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5281 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5341 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5401 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5461 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5521 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5581 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cagccttttt acggttcctg
     5641 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5701 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     5761 tagcccatat atggagttcc g
//
