LOCUS       sequence-468350-        9320 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression vector for Luciferase without v5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9320)
  TITLE     Janes Lab Plasmids 2025
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti PGK Blast LUC (w528-1)
FEATURES             Location/Qualifiers
     source          1..9320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..104
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             123..521
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             608..841
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    913..1034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(950..969)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1004..1023
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1074..1209
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1229..1248)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1230..1248)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1258..1275)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1258..1274)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1267..1289)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1416..1871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1503..1522)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1713..1734
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1897..2001
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2002..2070,2071..2862)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2220..2239)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3033..3621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3522..3541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3775..3792
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3909..3930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3945..3950,3951..3968,3969..3975)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3983..3999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3988..4010
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4007..4023
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4007..4023
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4042..4062
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4044..4062
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4090..4316
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4317..4497
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4544..4669
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5162..5395
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5580..5624
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5891..6008
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6054..6564
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6483..6501
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      6636..6645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6642..8294
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(6708..6726)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(8301..8325)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8356..8944
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8409..8429)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8827..8838)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8995..9015)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9000..9320,1..9)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9180..9315
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
       61 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
      121 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
      181 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
      241 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
      301 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
      361 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
      421 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
      481 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacaattc gagctcggta
      541 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
      601 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
      661 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      721 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      781 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      841 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
      901 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      961 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1021 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     1081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     1201 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     1261 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     1321 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     1381 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     1441 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1501 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1561 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     1621 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     1681 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     1741 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     1801 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     1861 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1921 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1981 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2041 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2101 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2161 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2221 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2281 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2341 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2401 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2461 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2521 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2581 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2641 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2701 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2761 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2821 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2881 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2941 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3001 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3061 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3121 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3181 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3241 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3301 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3361 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3421 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3481 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3541 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3601 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3661 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3721 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3781 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3841 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3901 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     3961 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     4021 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     4081 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     4141 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     4201 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     4261 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     4321 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4381 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4441 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     4501 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     4561 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     4621 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     4681 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     4741 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     4801 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     4861 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     4921 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     4981 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     5041 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     5101 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     5161 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     5221 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     5281 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     5341 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     5401 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     5461 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     5521 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     5581 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     5641 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     5701 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     5761 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     5821 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     5881 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     5941 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     6001 aaaattttat cgatcacgag actagcctcg agaagcttga tatcgaattc cacggggttg
     6061 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     6121 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     6181 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     6241 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     6301 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     6361 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     6421 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     6481 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     6541 cgaatcaccg acctctctcc ccagggggat ccatgggaac caattcagtc cctaggcttt
     6601 tgcaaaaagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca
     6661 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc
     6721 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     6781 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
     6841 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     6901 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     6961 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
     7021 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
     7081 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     7141 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
     7201 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     7261 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     7321 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     7381 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     7441 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     7501 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     7561 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     7621 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     7681 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     7741 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     7801 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     7861 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     7921 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     7981 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     8041 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     8101 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     8161 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     8221 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     8281 agatcgccgt gtaattctag acccagcttt cttgtacaaa gtggttgata tccagcacag
     8341 tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8401 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8461 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8521 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8581 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8641 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8701 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     8761 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8821 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8881 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8941 ccgcctggaa ttctgcagat atccggttag taatgagttt ggaattaatt ctgtggaatg
     9001 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9061 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9121 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9181 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9241 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     9301 gcttttttgg aggcctaggc
//
