LOCUS       sequence-469269-       10474 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Expresses SpRY_SlTH51 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10474)
  TITLE     PAM-interacting domain turn-helix 51 (TH51) motifs enable 
            modification of Cas9-SpRY activity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SpRY_SlTH51-IP
FEATURES             Location/Qualifiers
     source          1..10474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1250..2968
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5411..5431
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5495..6047
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5639..5656)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5866..5885
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6059..6658
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6059..6078)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6555..6575
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6684..7272
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6737..6757)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7155..7166)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7344..7577
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7655..7776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7692..7711)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7746..7765
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7816..7951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7971..7990)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7972..7990)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8000..8017)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8000..8016)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8123..8140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8294..8882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8374..8393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9053..9844,9845..9913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9676..9695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9914..10018)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10097..10116)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    10250..10321
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(10326..10372)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    10390..10438
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 ctagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcgt ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgtggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat cagcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg cccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagcac aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcca gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa aactacccct aaaagccaaa
     1201 agatctttgt cgatcctacc atccactcga cacacccgcc agcggccgca tggacaagaa
     1261 gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
     1321 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa
     1381 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgaga gaacccggct
     1441 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga
     1501 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
     1561 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
     1621 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
     1681 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
     1741 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
     1801 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
     1861 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa
     1921 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
     1981 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
     2041 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
     2101 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
     2161 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
     2221 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
     2281 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga
     2341 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga
     2401 cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac
     2461 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg
     2521 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct
     2581 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg
     2641 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa
     2701 gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa
     2761 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct
     2821 gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca
     2881 gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct
     2941 gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga
     3001 agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa
     3061 ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac
     3121 actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga
     3181 cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg
     3241 gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa
     3301 gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt
     3361 taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat
     3421 tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt
     3481 ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc
     3541 cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat
     3601 cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac
     3661 ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt
     3721 ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca
     3781 gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg
     3841 gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg
     3901 gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga
     3961 gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac
     4021 ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga
     4081 cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc
     4141 cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc
     4201 ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct
     4261 ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa
     4321 gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa
     4381 ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga
     4441 gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg
     4501 gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg
     4561 cttcagcaaa gagtctatca gacccaagag gaacagcgat aagctgatcg ccagaaagaa
     4621 ggactgggac cctaagaagt acggcggctt cctgtggccc accgtggcct attctgtgct
     4681 ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct
     4741 ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc
     4801 caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt
     4861 cgagctggaa aacggccgga agagaatgct ggcctctgcc aagcagctgc agaagggaaa
     4921 cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa
     4981 gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca
     5041 ctacctggac gagatcatcg agcagatcag cgagttctcc aagagaacca agctgataga
     5101 taagaagttg ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     5161 agagcaggcc gagaatatca tccacctgtt taccctgacc agactgggag cccctagagc
     5221 cttcaagtac tttgacacca ccatcgaccc caagcagtac agaagcacca aagaggtgct
     5281 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     5341 tcagctggga ggtgactctg gaggatctgg aggcagcaag agaaccgccg atggaagcga
     5401 gttcgagtct cccaagaaga agaggaaagt cggaagcgga taagctgcca agcttccgag
     5461 ctctcgaatt caaaggaggt accccccccc cctaacgtta ctggccgaag ccgcttggaa
     5521 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5581 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5641 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5701 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5761 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5821 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     5881 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5941 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     6001 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     6061 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6121 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6181 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6241 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6301 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6361 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6421 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6481 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6541 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6601 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa
     6661 acccgctgat cagcaaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6721 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6781 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6841 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6901 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6961 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7021 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7081 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7141 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7201 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7261 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     7321 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7381 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7441 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7501 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7561 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     7621 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7681 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7741 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7801 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7861 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7921 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     7981 agtcgtatta cgcgcgctca ctggccgtcg ttttacactc actgcccgct ttccagtcgg
     8041 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcgggaaga ggcggtttgc
     8101 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8161 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8221 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8281 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8341 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8401 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8461 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8521 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8581 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8641 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8701 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8761 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8821 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8881 aagaagatcc tttgatcttt tctacggggt ctgacactca gtggaacgaa aactcacgtt
     8941 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9001 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9061 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9121 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9181 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9241 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9301 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9361 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9421 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9481 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9541 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9601 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9661 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9721 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9781 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9841 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9901 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10021 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc
    10081 tagggtcgac tctcagtaca atctgctctg atgccgctcg actctggctc tatcgaatct
    10141 ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc
    10201 tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc ggctcccgac atcttggacc
    10261 attagctcca caggtatctt cttccctcta gtggtcataa cagcagcttc agctacctct
    10321 caattcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tatcaatcgt
    10381 tgcgttacac acacaaaaaa ccaacacaca tccatcttcg atggatagcg attttattat
    10441 ctaactgctg atcgagtgta gccagatctc gtga
//
