LOCUS       sequence-469285-       10968 bp    DNA     circular SYN 16-APR-2025
DEFINITION  For expression of hNM2B   in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: HA-hNM2B
FEATURES             Location/Qualifiers
     source          1..10968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8830..8856)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      8859..8868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8920..8939)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8921..8939)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8984..9187)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9013..9033)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9188..9567
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9739..9758
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9833..9937
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9938..10006,10007..10798)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10156..10175)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctctagactc gagcggcctt actctgactg
     2881 gggtggctgc gtctcgttga catcactggt cttactttct gtgtcatcgt cggagagctc
     2941 cagggaagct ccttcaaggt gcagctggcg ccggccagat cggctggaag agaagctgat
     3001 ggggccaccc cgcctcagcc ggttcttcag ggtgctgacc tcgcggctca ggccctcgtt
     3061 ggcctcggtg gcatcatcca gttcccgctg gagtttacgc cgagatgcgt tggcacgcgt
     3121 cgcttcttct tctgcttcct ccagctggcg tttaagctgc ttcatccgag cgttggcctt
     3181 ctccatctgc tctttatact ggtccgcgtg tcgacgctca tcctcaacct gcatgaagat
     3241 ttctttcagc ttcttctcag tgcgacggac taatttgttg gcggctgctc gttccttggc
     3301 ttcctgctca agctgctcct ccagctgccc aatcttggcc tccagggctg agatggtggc
     3361 cttgaactta gacttgacag caccctcgag ttcctgcagc ttggccttca gctccttgtt
     3421 ctgccgctcc agttgctggc gtgcattgtc actcttctgg gcggcgctgc gctcggctgc
     3481 tagctcggcg ttcagtgtgt ccacctgtag agtggtcttg cggaagcggt cgttgagcag
     3541 ctccatgttg ctctgctcct cttccagctc ctcctccagc tgtgcgatcc gagcttccag
     3601 acgccgcttc tcatccagca gcgcggactt gccagaggcg ctgttggtga tctcgtccgc
     3661 cagctcatct ctctcctgct cggcgtgtcg gcgggctcgc tcagatgagg caagttcctc
     3721 ctgcaattga aggatttctg cttccagact cttcaatttc ttttcactct ctttggattg
     3781 agcaaaaatc tcatctctgg atgcacgagc ttcttctaat tcacgttggt aatccttcat
     3841 ctgagcctgg agcttgcgga gctgcttaat cacctcatcc cgagctttgt tcgcagcctc
     3901 gatttgggct tcgaggtcct tcaggtctat ctccatcttt ttctttgaag ctacagcaag
     3961 cgcccgctgt ttcctctcat cctccagctc cgcctcgagc tcccgcacct gtttgatcag
     4021 cagccgcttc ttctcttcat tctgctcatc cctggtttgc aggtctctct cgaactgcgc
     4081 cttcatggcc tgcatgttga cctccagacg aagcttggca tcttccgtgg cctggagttc
     4141 gtcttccagc tcctccagct gggtcctcat ttcctccacc tgctgctcta gggcccgttt
     4201 ggatttttca agttcgtgaa cgttttttcc cacatcatct ttggagctca tgaggtcttc
     4261 catgtctgct cggagctgct tgttctgcct ctcaaactcc tccttggcct ccagggcttc
     4321 ctcgagggcc cgggccagtg acagggcttt ggtttctttc tctctggcct cggcttcggc
     4381 ccggtcccgc tcttcggcat agcgagcaga gatgctcttc tcttctgcta acagctggtc
     4441 aaacttcttc tgcttcttct ccaagttgga ggcgacctgg cgctggtggt ccaggtccac
     4501 cgtgaggtcg tccagctcct gctgcaggcg gttcttggtc ttctccagtt tgtcatacgc
     4561 cagtgccttc tcctccaggc gctggctcag ggcctccgcg tccttcagaa gcttcttctt
     4621 ggcttcttcc agactttcaa ttgttcccag gtcgtcatct actttcttct tggtatcagc
     4681 caactgggac tgcagggcca gcacttgctt ctccaggttc ttcctggcct cctcctcctc
     4741 ctcctgctgc tcctgaagac tgttcttctc ctcttccagc tgccggatcc gactgctcag
     4801 gtttagtttc tggcgtgtct cctcctgaag aagctcctgt gtatcctgta gttgagactc
     4861 aagactagct gcatccttag caaatttaat acccttcttc tctgcttctt ccagaagggt
     4921 ggagacatta tctagctcat tctgcagctt acttgctttc tccgccagct ccaccctgag
     4981 cctgtcgcct tcagagacct tggcatggag ctcctggacc tgcgcgtcga gcttcttcct
     5041 cttgtgctca gactcagcct tgacctgctg caggaccttc acctcacacg ccagctcctt
     5101 gttatctgtc tccaggccct gcttgttctt ctctagattt gctttgaacc gcttggcctg
     5161 ttccagctgc tctgagagct cctccagggc tgttgcgtgt ctttgtctca tgtcctggat
     5221 ttgagcttca tggttcttag tttcctcctc aagagctttc ttcagctctg ccacttcttg
     5281 ttcacgtttt gtacgtagtt cctgctgggc tgccgtggtg tccagcgtgt cctccagctc
     5341 tgttttcaga gcttccagtt cctcactcaa gtcccttttc tgcttttcgg ccttgttccg
     5401 tgaagccttc tcggattcaa agtcttcctg aagttcagca atttgggctt gtagctctcg
     5461 cacaacttta agggcattgt tcttatggag tgtttcatca tcacctctgg ccagtgcgcc
     5521 ctgcagctcc tcctccttct tggccagctg cagcttgagc tcatcaatct gcgcctgcag
     5581 ctctgcgatc tggtcctgca ggtcggtcgt ctccccgtcg agttttcttt tggccttttc
     5641 cagttcctga cgagtctttt cttccttctt taagcgttct tctagatctg agatcatcac
     5701 ttcttgctta ttcctgattt tggccaagtt tttcgccttt tcttcctctt cagccagctg
     5761 agaggaacac tcagcaatgc gatcttccat gagtttcttt tctttgatga acttggaatt
     5821 ttggtcctcg agaagcagaa tctcctcttc catcttcttg atcttggcct ctgctgtcac
     5881 cttttccagc tgcagctttt gccgagcccc ttcctcctcg tctagctgtt cttccaggtc
     5941 ctgaatatgt gcttgcattt ttttcttttc attttggagg atttggtttc tttcttcttc
     6001 ttcttcaacc ctagactcca agtcatgtag aatctcttct aattcctgct ttttagcagc
     6061 aagtcttgcc ctcatctctt ctgcttcagc aaagagctca gtctctgctt gtagttgttc
     6121 tgcaaggata ttcttctctt ctaaaagctg ctggtgcttc cgctccatct cctccagctc
     6181 tccttccacc tttgtctgct tctccttcac cttcaacagc tcttcatctt tggcctgaag
     6241 ttcttcctcc tggcgagtca cttgtagaag cggcttcacc tttgtgaaga ctcgccacca
     6301 ctgccagtgc cgtaatttca ggtacgcggc acagttccgc tgcaagacct ttaaggcact
     6361 tagttgctgc tgcttcttgg caaaggcctt tctggccagg taacctctgc aaacggcctg
     6421 gaagaagata atgatatcgg tgatttttaa atctctttct tcctctaagt gtgccagaac
     6481 tccagctctg aaaaatatct tgctctgtcc aattctgtac aagtttgggt ccaattctaa
     6541 agcccggatc attcgttcac aggcctgttt accatccata aaacctttag gaatagcatt
     6601 tggagttagg atctcatatc tctgtctgaa ttcctggaaa actattcggt tagggaagcc
     6661 ctggcgacag attcggatcc cttccaggac accattacag cgaagctgat ctaggactag
     6721 gtgtggatcc aattttccag ccctcttctc gtgatttgga atgatacaac gaacaaagtt
     6781 agggttggtg tttcggagag ttgccatcag cttggtgaga gattctttgt agagttgccc
     6841 aacggtacga aacatgccct tcttggtttt atatgcggag ccaaaagctg tctcagtcat
     6901 accagtgact tgatccagac ccacgatacg gtccacatct ttccaaagct ctgccacaaa
     6961 tctgtctgat gactggtgca aaagggtggc cacgttgtca ttcagggggt ccatattctt
     7021 catcagccac tcatctgcct tatagtccac cttccctgca taatgtataa tgcaaaaatc
     7081 agctttgtct tttaattgtc gaggtttctg aaacttggag tgggaacctt gctcttgaac
     7141 cagtttttca acaaaggttt tatctgtggc tttagggaac cagcattctt catccaaaag
     7201 ggccagtaca ccaggagggt tcgcaggtct ctctattagg tcgatgcatg gctgcagatc
     7261 cagcccgaaa tcgatgaagt tccactcgat gccttcgcgc tggtattcct cttgttctag
     7321 gataaacatg gtgtggttga acagctgctg cagcttctca ttggtgtagt tgatgcaaag
     7381 ttgttcaaag gagttcagct caaaaatttc aaatccagca atatccagga ttccaatgaa
     7441 agatgctccc tgacgtttgg tcctatccag agctttattg atgcgatgaa cgagccagcg
     7501 aaagagccgc tcataggtag cttttgccaa tgcttctact gcaaaatctg cctgttcttt
     7561 ggtctgggct ttttgcacat agtctcggcc gaccttgatc cggggagtca ggatggcccg
     7621 agtaaactcc atcacattca tcccaagaag atggcagagc ttctgcgcaa ctgtattttc
     7681 tggcatggaa gcttgatcag tatttctctc ctttttgaaa gaaatatttc caaactgtag
     7741 cactgaagat actactttaa gcattgacag aatctcttca tgggagaagc ccattatgtg
     7801 cattgcttcc atggtctcct ggaaattatc tttgtcttgc tgtcccggaa taggaatata
     7861 gccattggag agaaacctgt agttattaaa tccttcaaga agcaaatcag actttaggtg
     7921 ttctcctgct ccagataaca actggtaaaa gatatgaaaa gtacgttcat cttttgcttg
     7981 acgaacagca cgagactttt ccagaaggta tgtttcaatg ttggccccaa cgatatagcc
     8041 agttacatca aagttgatcc gaataaattt gccaaaacga gatgagttat catttttcac
     8101 agtcttcgca tttccaaatg attccagaat tggatttgct tgcaaaagct gccgttcaag
     8161 ttccccagga atattatggt cctttcttcc tttatgtgaa gaagcaacat gggcaaggta
     8221 ctgaataact ttctttgtat tttctgtctt cccagcacct gactcacccg tgcaaagaat
     8281 tgactggtcc tcacgatctt gaagcatgca tctgtaagca gattcagata tagcatagat
     8341 gtgtggaggc atctcatgac gcttcttccc tctgtacatt tcaataatat tctcagagta
     8401 aattggaaga ttcttgtaag ggtttataac tacacagaag agtccagaat aagtatagat
     8461 tagtcctgaa tagtagcgat ccttcagatt atgtaaaacg gaagcttcat tcaagcatgt
     8521 caattctgcc atatcctcca ccttggaaaa cttaggtggg ttcatcttct gaatatcatc
     8581 tttgttgacc attgctttct ttccattctc tgccaactcc accataactt catctccccg
     8641 ttcttctttg atactagctg cctcaaaacc atggcgttct gatggaatcc acactagctt
     8701 tttagctgtc caatcagctt gagtggcagg gttgtagatg acagccctgt ccacaaagag
     8761 atacctctct ggatcctcga gtccagttct ctgcgcgcca gatccgctgc cactgcctga
     8821 cgcaccccca gcgtagtctg ggacgtcgta tgggtaggcc atggtggcga tacgagctcg
     8881 gtaccaagct taagtttaaa cgctagccag cttgggtctc cctatagtga gtcgtattaa
     8941 tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga
     9001 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     9061 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     9121 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     9181 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     9241 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     9301 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     9361 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     9421 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     9481 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     9541 attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca
     9601 gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg
     9661 ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca
     9721 gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat
     9781 cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     9841 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9901 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9961 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10021 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10081 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10141 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10201 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10261 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10321 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    10381 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10441 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10501 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10561 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10621 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    10681 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10741 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10801 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10861 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10921 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
