LOCUS       sequence-469301-        9629 bp    DNA     circular SYN 16-APR-2025
DEFINITION  For expression of cParacingulin L1  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: GFP-cCGNL1-HA
FEATURES             Location/Qualifiers
     source          1..9629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2873..2899)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6482..6493
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(join(6806..7516,7517..7519,7520..7522))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6848..6869)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7197..7216
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7456..7477
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7519..7528
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7581..7600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7582..7600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7645..7848)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7674..7694)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7849..8228
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8400..8419
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8494..8598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8599..8667,8668..9459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8817..8836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctctagactc gaggtcgacc taagcgtagt
     2881 ctgggacgtc gtatgggtag aattcatcga cgatctggct ggtggcagcg ctgtccttgg
     2941 ggaaggtgta gctcagcggc gcctcgtgga ggctcccccc gtccgtactg aggtcatcat
     3001 catccgtgtc gtccagcact ttgctcggca gcttcttgag tcttaggtcc ttcttcatgg
     3061 aattgagttg tccctgcagc tgctcattca catccatctg ctcctccagc tccctctgca
     3121 gcttcttttt agaactttct agtctgtcga tctcctcctc ggcctcctct acttgtcgtt
     3181 tcattgcttt caagcgcaag ctaagctggt ccttctgatc agtcagagac aggtgctcat
     3241 cgtccacttg catcactagc tccttcacct tccgctccag ccgtcggttg ctgagctgga
     3301 ggctggcccg gtccctctcc tcgttttcca gacggtcctc cagctccgcg atcctggcct
     3361 ccatctgcac caccagcccc tctttgctgg acctgtagga gccttccaga tggataatcc
     3421 ggctctttaa atctttgttc tgcctctcca aggaaatctt gtcacactcc aagtcctgct
     3481 tgagagctct ttcctgcagg agctcgctcc tcatctgctc catctgttcc ctgctccgag
     3541 tgatcctctc cgagagcaaa tccgagttat ttctttcttc ttccagttcc atctccagct
     3601 gagacacctt gtcctccatc tgcttgacga gtctgcttcg gtcatctttg aggtgacttt
     3661 tggcctccag ctcgtattcc aggtccttga gtgtctgctc cagaagctgc ctttttgtga
     3721 gcgcctcatc ctgagctctc tggtagtccc gtaactcctc ctccatcaga cgcatctcat
     3781 cctgcagttt catggcagtc agacgtgact tttcagcctc tagcgttttc tccttcaact
     3841 gcctttgcat ttctgcaagt tccctgcgat ttttctcctt gtactcatct agctggttct
     3901 ggagctccag tgttgataca cgggacacct caacaatgtc agccatctct ttctgcaatt
     3961 tttcaatggt cttgtccaga tgccatcgct cgttctccat ctcgttcttc agcctcctta
     4021 actgctcttt ctgctcagtc tcatctttaa gcttctcaga caactgcttc tgctcctggg
     4081 tggcccgact cagatttctc tgcacgtctt ccagctcacc ctccagggtc cttctgactg
     4141 atgtggcttc tctttcttcc tttcttgcat tcacaagggc ctcctctaat tgccggtttt
     4201 ctctctcgca ttttgtgagc ctttcctttg ccttgacctc atcgtccttc atgtcagcaa
     4261 tctgcctctg cagccgagcg acgctctgct ccagctcttc gatgctttcc cgcagcttct
     4321 cattctcctc ctgcagagcc ttcacgtgca tttcagcccc cagctggctc tgctctgaag
     4381 tgttgctccg actggccagg acctcgacgt tcttggttgc ttcttctaca ctctccctca
     4441 gggcctgcag ctcggcgtca tactgctcct tcagcttatc catctccagg tcgtggctgg
     4501 acacctcttc cttcagggct cccttcaggg cggtcagttc acgctcccgc ttcctcaaga
     4561 ggtcctcctg ctcctctttg gcgatgagca gatcctgaag atcctgtttt gcctgtaaga
     4621 gttcctcaat cagggctccc ttttccctgt cctcagatcg tttagcactg tccaattcat
     4681 cgtgcatttc tgagagctgg tcctggagat ccctgatctc agtctggtgc tgttcccgtt
     4741 ccatcttcac ctggaacaat tcctccaggt tcttgcggag ctccccttca ctctcttcca
     4801 ggcgctgctg tagcatggag ttctcttcca ctttactatc atgctggccc tggagctcct
     4861 ccaaatttgc tttcattctc tctctctctt ctttgatgtt ctgctgattc ttgacttcta
     4921 gttgaagctg tctctgaagt tcagccactt ctgtggtcag cttgtttttc tgttccagca
     4981 gatctctgac ttcagatgaa gaattagaag cttgattatt tccttgtgtg ctccctgctg
     5041 ctcgagactt taaagtctgg attttctcaa agaccaggtt gactttccgt ttagtcgcat
     5101 catcattatc ggtgcttcct tctttgaggt aattgtaaag tatctgcttg gccgtctcct
     5161 cattggtttg ttgagtgagc tcttgttggc ctttcaacag atctggtgtg gcctgagtgt
     5221 tggaggcgga agtaaatgtc ttcacagaaa tcttcttggc accagagtcg ggcgaagtcg
     5281 ctagggcccg attctgtaac atcagcgtcg ccgtggctgt cttgatttcg tcctcctttt
     5341 tattctgggc tgggggggag gacacacgcg tgtttgtact ctcccgaccc cctctgtttg
     5401 ctggagcttt cccatctttg ctctgctggg caggccggag gtgggccccc tgcagcttgg
     5461 cgaaggcgcg gcccacgctc tgtctcccgc ggtgctcctg ggcaggtgac cgctgcgggc
     5521 acacctgcga cgggcggagg aggtgttcgc tggacttgcc caggttccgg ctaaattcgc
     5581 tgaggtactc cgagttcccc tgcagaccga aaggaaaggc actatccacg cttctggacc
     5641 tcttcctgtc ttctggattg attctgttcc gcttcccagg ccttcctctc ctctgaagcc
     5701 cgggtttttg gtcaaacttt tcaatcagtt gatccacacc aggaattgat cccgtgtcaa
     5761 tatcccgccc agttcctggc aggaagggga tataccgtct gttttcgtgg cgattcacat
     5821 tgtcagcgtg gatggcacat tcctgatcat caagcagaaa tctgtacaaa gagttggccg
     5881 acgtgggggt tgccgacgag gaagaggacc tccgggaccg agctccgtcc aggacaggtc
     5941 ccgctgaatc ttgtcgccgg aagggaagca cgtccggcct ggctttcttg gcttcaggat
     6001 gggtgtgcgg gctgtgggca gtgggggacc tcccactagg ggaaggggcc tcccctacca
     6061 gcgcctcccc ggtgcagctc tcaacattta cgcacactga ggtctgcttc ttggggtcat
     6121 cgatgaccac agagctgcac aaacggatgg ccgtcacgcc ggatttgccc gcatcttcca
     6181 aaggagggct ggtaggctgg gaactaccag gcttggggaa gcaggtccat gccttcttat
     6241 tgttggtacc ttcttctgtc aatgtgttta gccaattatg ctcaggaggc tggtggtttt
     6301 gcaagttcga ttcattcttt tcagggtcat agggctgcag aagctctgga tgcctctgaa
     6361 agttcaacac gtgggatggc ttcattggcc cctctttgcg ttctagaacc ccgttcttgc
     6421 cttcgaagag agaggactgt ttcaggtttc ttactgggct gggctgggca tacggatttt
     6481 ctgggagctg caattcctct gcgctgctct cctccagcac ggacccgttg ctggaatgca
     6541 gaggcaggtt acttctcatt ggagatggaa atggtggccc attttcagga aaagatgtgc
     6601 ctgccagaca ccgctctgtg ttattcagga ctatgtaggg gtgaccatcg attccctgca
     6661 cccgaatact gacaccatag gagcctgcct tagagtgttg ggaattcctt ggctttttgc
     6721 tatcatcgct tgcgagtctc agatggacgc catattcttg ctgtacgtgt tggtattcgc
     6781 cgaaatacag ctccatgcgg ccgctcttgt acagctcgtc catgccgaga gtgatcccgg
     6841 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     6901 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     6961 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     7021 tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag acgttgtggc
     7081 tgatgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     7141 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     7201 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     7261 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     7321 cccaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     7381 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     7441 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     7501 gctcctcgcc cttgctcacc atggtggcgg atccgagctc ggtaccaagc ttaagtttaa
     7561 acgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc
     7621 agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
     7681 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
     7741 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     7801 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
     7861 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     7921 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     7981 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     8041 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     8101 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     8161 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa
     8221 tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct
     8281 aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct
     8341 cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc
     8401 ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg
     8461 tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8521 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8581 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8641 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8701 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8761 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8821 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8881 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8941 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9001 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9061 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9121 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     9181 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9241 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9301 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9361 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9421 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9481 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9541 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9601 gaccccgtag aaaagatcaa aggatcttc
//
