LOCUS       sequence-469716-        4741 bp    DNA     circular SYN 23-APR-2025
DEFINITION  Gateway ORF Entry clone of mouse Acvr1 with stop codon (for native 
            or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4741)
  TITLE     Esposito lab plasmids 2025
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 8061-E40
FEATURES             Location/Qualifiers
     source          1..4741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             670..690
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2222..2321)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2338..2357)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2339..2357)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2362..2378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2362..2378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2809..3600
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3722..3831
                     /note="incP origin of transfer"
     rep_origin      4091..4679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4580..4599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tttgtacaaa
      661 aaagttggcg aaaacctgta cttccaaggc accatggtcg atggagtaat gatccttcct
      721 gtgctaatga tgatggcttt cccttccccg agtgtggaag atgagaagcc caaggtcaac
      781 cagaaacttt acatgtgtgt gtgtgagggc ctctcctgcg ggaacgagga ccactgtgaa
      841 ggccagcagt gtttttcttc tctgagcatc aacgatggct tccacgtcta ccagaagggc
      901 tgctttcagg tttatgagca ggggaagatg acgtgtaaga ccccgccgtc acctggccag
      961 gctgtggagt gctgccaagg ggactggtgt aacaggaaca tcacggccca gctgcccact
     1021 aaagggaagt ccttccccgg aacacagaat ttccacctgg aagttggcct tatcatcctc
     1081 tcggtggtgt ttgcagtatg tcttttagct tgcatccttg gagttgctct caggaagttt
     1141 aagagacgca atcaagagcg cctgaacccc agagacgtgg agtatggtac cattgaaggg
     1201 ctcatcacca ccaatgtggg agacagcact ctagcggaac tactagatca ctcgtgtaca
     1261 tcaggaagtg gctccggtct tcctttcctg gtacagagaa cggtggctcg ccagataacc
     1321 ctgttggagt gtgtcgggaa gggccggtat ggagaagtat ggaggggcag ctggcaaggc
     1381 gaaaatgtcg ctgtgaagat cttctcctcc cgagacgaga agtcatggtt cagggagacg
     1441 gaattgtaca acactgtgat gttgaggcat gaaaatatct taggtttcat cgcttcagac
     1501 atgacctcca gacactccag tacccagctg tggctcatca cacattacca tgaaatggga
     1561 tcgttgtatg actaccttca gctcactact ctggatacgg ttagctgcct tcggattgta
     1621 ctgtccatag ccagcggcct tgcccatttg cacatagaga tatttgggac ccaagggaag
     1681 tccgccattg cccatcgaga tctgaagagc aaaaacatcc tggtgaagaa gaatggacag
     1741 tgctgcatag cagatttggg cctggcagtc atgcattccc agagcacaaa ccagcttgat
     1801 gtgggaaaca acccccgtgt ggggaccaag cgctacatgg ctccggaagt gctcgatgaa
     1861 accatccaag tggattgctt tgattcttat aagagggtcg atatttgggc ctttggcctt
     1921 gttctgtggg aagtggccag gcgaatggtg agcaatggta tagtggaaga ttacaagcca
     1981 ccattctatg atgtggttcc caatgaccca agttttgaag atatgaggaa agttgtctgt
     2041 gtggatcaac agaggccaaa catacctaac agatggttct cagacccgac attaacttct
     2101 ctggcgaagc tgatgaaaga gtgctggtat cagaacccat ccgcaagact cacagctcta
     2161 cgtatcaaaa agactttgac caaaatcgat aattccctag acaaattaaa aactgactgt
     2221 taatcaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2281 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2341 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2401 aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc
     2461 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
     2521 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     2581 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     2641 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     2701 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     2761 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg
     2821 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     2881 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     2941 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     3001 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     3061 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     3121 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     3181 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     3241 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     3301 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     3361 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     3421 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     3481 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     3541 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     3601 ccctcgactc tagaggatcc ccgggtaccg agctcgaatt ccgtgatagg tgggctgccc
     3661 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     3721 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     3781 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     3841 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     3901 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     3961 gaaaacggaa ttcgagctcg gtacccgggg atcctctaga gtcgagccac ccatgaccaa
     4021 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     4081 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4141 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4201 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4261 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4321 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4381 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4441 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4501 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4561 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4621 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4681 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4741 t
//
