LOCUS       sequence-470157-       11991 bp    DNA     circular SYN 25-APR-2025
DEFINITION  To express tagBFP-2A-FLAG-coORF8 (co = codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11991)
  TITLE     Manel lab plasmids 2025
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTRIP-SFFV-tagBFP-2A-FLAG-coORF63
FEATURES             Location/Qualifiers
     source          1..11991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      4498..4507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4507..4530
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6395..6575
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8103..8122)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8336..8355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8455..8477
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8515..8533)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8601..8705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8706..8774,8775..9566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8924..8943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9737..10325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10226..10245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10479..10496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10566..10586)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10571..10900
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10751..10886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc cactagtgcc
     4501 accatggatt ataaggatga cgatgacaag gctacagtcg ctgaaagatg tcccatatgt
     4561 cttgaagatc catctaatta tagtatggct ttgccctgtc tccacgcctt ttgttatgtc
     4621 tgcatcacca ggtggatcag gcagaatcca acttgccccc tgtgtaaggt cccagttgag
     4681 tcagttgtac atacaattga atctgattct gaattcaagg agaccaaagt ctccgtagat
     4741 ttcgattacg atagtgaaga ggatgaggat tctttcgaag gacagttcct tgcggtagac
     4801 agcggtgacg cccctgcaaa catatccgcc tggaatggcc caatggcctt cgtcccactt
     4861 aatgccaacg gcacagcagg cgcacctaga ctccaacccc tcgttgattg gctcgtagag
     4921 cgattagatc aactgtttga aacacccgaa ctggctctcg taatgcgaaa tatagtgatg
     4981 gacaccttgt gtgaacacgg gtgcaatgaa gaggaactga ccaggcaatt ctggcccatg
     5041 ttccatgagg ataccgttcc tttcgttacc gacctgattg ttcaggccga gctctgtgtc
     5101 gcaagccggc caatactccc tatcgcacgg ggccgcggag tggagtatat cgatagtagc
     5161 tcttcttcta gtagctccga agaggagacc gatagtgata tcgaagtaga tccaaataat
     5221 ctgacagatc ccgaggacac aagtgacgag acatcaaccg ataacagcag tgcacaagcc
     5281 ccacgacagg aagattctcg acccgcccgg gctagacctg gaccccctac tcgcggtagg
     5341 cggcgcggcc gccgcccagc agcacccggc cctgcatcca ggcgatctgc gcggctccgt
     5401 aggcgtcaac cgaggacaaa tagtagaact aatggcggtg ataatggtga gatcatcgat
     5461 ctgaccctcg attctgacgg tgatacagag cccgcagacg tgtccggttc tctcaacacg
     5521 actgaccaac ctgtcctgat tccggatgaa gaggaagccg ccccagccag cccgcacacc
     5581 agtagtaata gcgctatcat atgtttggta tcagagttga ccccagaatc cgaggagcca
     5641 cctagagatc agcctgtagc gccttcagga tcatccgcag gagaacgccc tatgaggcca
     5701 cgatgttctc tcagggaatt cgcaaggcgc tttatggcct tggccccaag ggatagtagc
     5761 acatctgagg cagccggtcc ttctaggttg ggcgccgggc cacgggccac cgaaccattt
     5821 agtgtcgccg tggtgctggt ggatcggagt tcagaaggtg ccggactctt cggcggccga
     5881 tttgcccaac atgtcagacg ccgtacagaa gacgagtctg cgcggagaag aggaaacgta
     5941 ctgttgcgcc ccaggagaca atcagtccca ccagtacctt atcccgatat cgccagcact
     6001 tcacctctga tacgccaggg cggacaacgg gtgcgagatc tccagcgagc cttccaaacc
     6061 caaccagccg agccggaaga aatgaggtgc cctcataatt gtcagcgtta taggcgaaat
     6121 cagtagctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     6181 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     6241 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6301 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaaaat cgtcgagaga
     6361 tgctgcatat aagcagctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6421 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6481 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6541 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6601 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattataa
     6661 tagatttagc aggaattgaa ctaggagtgg agcacacagg caaagctgca gaagtacttg
     6721 gaagaagcca ccagagatac tcacgattct gcacatacct ggctaatccc agatcctaag
     6781 gattacatta agtttactaa catttatata atgatttata gtttaaagta taaacttatc
     6841 taatttacta ttctgacaga tattaattaa tcctcaaata tcataagaga tgattactat
     6901 tatccccatt taacacaaga ggaaactgag agggaaagat gttgaagtaa ttttcccaca
     6961 attacagcat ccgttagtta cgactctatg atcttctgac acaaattcca tttactcctc
     7021 accctatgac tcagtcgaat atatcaaagt tatggacatt atgctaagta acaaattacc
     7081 cttttatata gtaaatactg agtagattga gagaagaaat tgtttgcaaa cctgaatagc
     7141 ttcaagaaga agagaagtga ggataagaat aacagttgtc atttaacaag ttttaacaag
     7201 taacttggtt agaaagggat tcaaatgcat aaagcaaggg ataaattttt ctggcaacaa
     7261 gactatacaa tataacctta aatatgactt caaataattg ttggaacttg ataaaactaa
     7321 ttaaatatta ttgaagatta tcaatattat aaatgtaatt tacttttaaa aagggaacat
     7381 agaaatgtgt atcattagag tagaaaacaa tccttattat cacaatttgt caaaacaagt
     7441 ttgttattaa cacaagtaga atactgcatt caattaagtt gactgcagat tttgtgtttt
     7501 gttaaaatta gaaagagata acaacaattt gaattattga aagtaacatg taaatagttc
     7561 tacatacgtt cttttgacat cttgttcaat cattgatcga agttctttat cttggaagaa
     7621 tttgttccaa agactctgaa ataaggaaaa caatctatta tatagtctca cacctttgtt
     7681 ttacttttag tgatttcaat ttaataatgt aaatggttaa aatttattct tctctgagat
     7741 catttcacat tgcagataga aaacctgaga ctggggtaat ttttattaaa atctaattta
     7801 atctcagaaa cacatcttta ttctaacatc aatttttcca gtttgatatt atcatataaa
     7861 gtcagccttc ctcatctgca ggttccacaa caaaaatcca accaactgtg gatcaaaaat
     7921 attgggaaaa aattaaaaat agcaatacaa caataaaaaa atacaaatca gaaaaacagc
     7981 acagtataac aactttattt agcatttaca atctattagg tattataagt aatctagcca
     8041 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     8101 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     8161 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     8221 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     8281 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     8341 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8401 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8461 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     8521 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8581 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8641 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8701 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8761 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8821 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8881 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8941 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9001 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9061 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9121 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9181 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9241 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9301 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9361 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9421 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9481 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9541 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9601 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9661 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9721 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9781 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9841 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9961 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10021 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10081 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10141 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10201 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10261 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10321 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10381 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10441 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10501 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10561 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
    10621 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
    10681 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
    10741 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    10801 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    10861 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
    10921 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
    10981 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
    11041 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
    11101 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
    11161 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
    11221 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
    11281 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
    11341 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
    11401 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
    11461 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
    11521 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
    11581 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
    11641 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
    11701 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
    11761 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
    11821 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
    11881 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
    11941 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt g
//
