LOCUS       sequence-47018-e         906 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Dittmer PJ, Miranda JG, Gorski JA, Palmer AE
  TITLE     Genetically encoded sensors to elucidate spatial distribution of 
            cellular zinc.
  JOURNAL   J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10.
  PUBMED    19363034
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2009-06-12"; volume: "284(24)"; pages: "16289-97. 
            Epub 2009 Apr 10"
COMMENT     Sequence Label: pcDNA-mito-ZifCY1
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..177
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             199..273
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             295..369
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(400..402,403..405,406..864)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(445..466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(706..725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtcggtctga cgccgctgct gctgcggggc ttgacaggct tgacaggctc ggcccggcgg
       61 ctcccagtgc cgcgcgccaa gatccattcg ttgggagatc ctatgtccgt cctgacgccg
      121 ctgctgctgc ggggcttgac aggctcggcc cggcggctcc cagtgccgcg cgccaagatc
      181 cattcgttgg gagatcctat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc
      241 tcggcccggc ggctcccagt gccgcgcgcc aagatccatt cgttgggaga tcctatgtcc
      301 gtcctgacgc cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg
      361 cgcgccaaga tccattcgtt ggggaagctt ggggatccca tggtgagcaa gggcgaggag
      421 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacagg
      481 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      541 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
      601 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      661 gccatgcccg aaggctacgt ccaggagcgt accatcttct tcaaggacga cggcaactac
      721 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      781 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
      841 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc ccacttcaag
      901 atccgc
//
