LOCUS       sequence-470271-       12452 bp    DNA     circular SYN 01-MAY-2025
DEFINITION  Express TAZ-CAMTA1 fusion gene in mammalian cells using the 
            doxycycline-inducible TRE promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Driskill JH, Zheng Y, Wu BK, Wang L, Cai J, Rakheja D, Dellinger M, 
            Pan D
  TITLE     WWTR1(TAZ)-CAMTA1 reprograms endothelial cells to drive epithelioid 
            hemangioendothelioma.
  JOURNAL   Genes Dev. 2021 Apr 1;35(7-8):495-511. doi: 10.1101/gad.348221.120. 
            Epub 2021 Mar 25.
  PUBMED    33766984
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2021-04-1"; volume: "35(7-8):495-511. doi"; pages: " 
            10.1101/gad.348221.120. Epub 2021 Mar 25"
COMMENT     Sequence Label: pCW-TAZ-CAMTA1
FEATURES             Location/Qualifiers
     source          1..12452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      543..552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             552..575
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             582..605
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        5394..5904
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5823..5841
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5914..6510
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5914..5933)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6410..6430
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6511..6564
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6565..7182,7183..7188,7189..7224,7225..7227,7228..7263,
                     7264..7266,7267..7302,7303..7311)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7351..7371)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7769..7780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7985..8218
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8290..8411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8327..8346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8381..8400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8451..8586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8586..8657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8658..8726,8727..9518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8876..8895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9689..10277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10178..10197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10431..10448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10565..10586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10601..10606,10607..10624,10625..10631)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10639..10655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10644..10666
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10663..10679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10663..10679
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10698..10718
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10700..10718
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10746..10972
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10973..11153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11200..11325
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11818..12051
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12236..12280
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat ggattataaa gatgatgatg ataaaggggc agactacaag gacgacgacg
      601 acaagaatcc ggcctcggcg ccccctccgc tcccgccgcc tgggcagcaa gtgatccacg
      661 tcacgcagga cctagacaca gacctcgaag ccctcttcaa ctctgtcatg aatccgaagc
      721 ctagctcgtg gcggaagaag atcctgccgg agtctttctt taaggagcct gattcgggct
      781 cgcactcgcg ccagtccagc accgactcgt cgggcggcca cccggggcct cgactggctg
      841 ggggtgccca gcatgtccgc tcgcactcgt cgcccgcgtc cctgcagctg ggcaccggcg
      901 cgggtgctgc gggtagcccc gcgcagcagc acgcgcacct ccgccagcag tcctacgacg
      961 tgaccgacga gctgccactg cccccgggct gggagatgac cttcacggcc actggccaga
     1021 ggtacttcct caatcacata gaaaaaatca ccacatggca agaccctagg aaggcgatga
     1081 atcagcctct gaatcatatg aacctccacc ctgccgtcag ttccacacca gtgcctcaga
     1141 ggtccatggc agtatcccag ccaaatctcg gagctggcgg cagcgtgcat cacaagtgta
     1201 acagcgccaa acaccgcatc atctcgccca aggtggagcc acggacaggg gggtacggga
     1261 gccactcgga ggtgcagcac aatgacgtgt cggagggcaa gcacgagcac agccacagca
     1321 agggctccag ccgtgagaag aggaacggca aggtggccaa gcccgtgctc ctgcaccaga
     1381 gcagcaccga ggtctcctcc accaaccagg tggaagtccc cgacaccacc cagagctccc
     1441 ctgtgtccat cagcagcggg ctcaacagcg acccggacat ggtggacagc ccggtggtca
     1501 caggtgtgtc cggtatggcg gtggcctctg tgatggggag cttgtcccag agcgccacgg
     1561 tgttcatgtc agaggtcacc aatgaggccg tgtacaccat gtcccccacc gctggcccca
     1621 accaccacct cctctcacct gacgcctctc agggcctcgt cctggccgtg agctctgatg
     1681 gccacaagtt cgcctttccc accacgggca gctcagagag cctgtccatg ctgcccacca
     1741 acgtgtccga agagctggtc ctctccacca ccctcgacgg tggccggaag attccagaaa
     1801 ccaccatgaa ctttgacccc gactgtttcc ttaataaccc aaagcagggc cagacgtacg
     1861 ggggtggagg cctgaaagcc gagatggtca gctccaacat ccggcactcg ccacccgggg
     1921 agcggagctt cagctttacc accgtcctca ccaaggagat caagaccgag gacacctcct
     1981 tcgagcagca gatggccaaa gaagcgtact cctcctccgc ggcggctgtg gcagccagct
     2041 ccctcaccct gaccgccggc tccagcctcc tgccgtcggg cggcggcctg agtcccagca
     2101 ccaccctgga gcagatggac ttcagcgcca tcgactccaa caaggactac acgtccagct
     2161 tcagccagac gggccacagc ccccacatcc accagacccc ctccccgagc ttcttcctgc
     2221 aggacgccag caaacccctc cccgtcgagc agaacaccca cagcagcctg agtgactctg
     2281 ggggcacctt cgtgatgccc acggtgaaaa cggaggcctc gtcccaaacc agctcctgca
     2341 gcggtcacgt ggagacgcgg atcgagtcca cttcctccct ccacctcatg cagttccagg
     2401 ccaacttcca ggccatgacg gcagaagggg aggtcaccat ggagacctcg caggcggcgg
     2461 aagggagcga ggtcctgctc aagtctgggg agctgcaggc ttgcagctct gagcactacc
     2521 tgcagccgga gaccaacggg gtaatccgaa gcgccggcgg cgtccccatc ctcccgggca
     2581 acgtggtgca gggactctac cccgtggccc agcccagcct cggcaacgcc tccaacatgg
     2641 agctcagcct ggaccacttt gacatctcct tcagcaacca gttctccgac ctgatcaacg
     2701 acttcatctc cgtggagggg ggcagcagca ccatctatgg gcaccagctg gtgtcggggg
     2761 acagcacggc gctctcacag tcagaggacg gggcgcgggc ccccttcacc caggcagaga
     2821 tgtgcctccc ctgctgtagc ccccagcagg gtagcctgca gctgagcagc tcggagggcg
     2881 gggccagcac catggcctac atgcacgtcg ccgaggtggt ctcggccgcc tcggcccagg
     2941 gcaccctagg catgctgcag cagagcggac gggtgttcat ggtgaccgac tactccccag
     3001 agtggtctta cccagaggga ggagtgaagg tcctcatcac aggcccgtgg caagaagcca
     3061 gcaataacta cagctgcctg tttgaccaga tctcagtgcc tgcatccctg attcagcctg
     3121 gggtgctgcg ctgctactgc ccagcccatg acactggtct tgtgacccta caagttgcct
     3181 tcaacaacca gatcatctcc aactcggtgg tgtttgagta caaagcccgg gctctgccca
     3241 cgctcccttc ctcccagcac gactggctgt cgttggacga taaccagttc aggatgtcca
     3301 tcctggaacg actggagcag atggagagga ggatggccga gatgacgggg tcccagcagc
     3361 acaaacaggc gagcggaggc ggcagcagtg gaggcggcag cgggagcggg aatggaggga
     3421 gccaggcaca gtgtgcttct gggactgggg ccttggggag ctgctttgag agccgtgtgg
     3481 tcgtggtatg cgagaagatg atgagccgag cctgctgggc gaagtccaag cacttgatcc
     3541 actcaaagac tttccgcgga atgaccctac tccacctggc cgctgcccag ggctatgcca
     3601 ccctaatcca gaccctcatc aaatggcgta caaagcacgc ggatagcatt gacctggaac
     3661 tggaagttga ccccttgaat gtggaccact tctcctgtac tcctctgatg tgggcgtgtg
     3721 ccctagggca cttggaagct gccgtcgtgc tgtacaagtg ggaccgtcgg gccatctcga
     3781 ttcccgactc tctaggaagg ctgcctttgg gaattgccag gtcacggggt catgtgaaat
     3841 tagcagagtg tctggagcac ctgcagagag atgagcaggc tcagctggga cagaacccca
     3901 gaatccactg tcctgcaagc gaagagccca gcacagagag ctggatggcc cagtggcaca
     3961 gcgaagccat cagctctcca gaaataccca agggagtcac tgttattgca agcaccaacc
     4021 cagagctgag aagacctcgt tctgaaccct ctaattacta cagcagtgag agccacaaag
     4081 attatccggc tcccaaaaag cataaattga accctgagta cttccagaca aggcaggaga
     4141 agctgcttcc cactgcactg agtctggaag agccaaatat caggaagcaa agccctagtt
     4201 ctaagcagtc tgtccccgag acactcagcc ccagtgaagg agtgagggac ttcagccggg
     4261 aactctcccc tcccactcca gagactgcag catttcaagc ctctggatct cagcctgtag
     4321 gaaagtggaa ttccaaagat ctttacattg gtgtgtctac agtacaggtg actggaaatc
     4381 cgaaggggac cagtgtagga aaggaggcag caccttcaca ggtgcgtcca cgggaaccaa
     4441 tgagtgtcct gatgatggct aacagagagg tggtgaatac agagctgggg tcctaccgtg
     4501 atagtgcaga aaatgaagaa tgcggccagt ccatggatga catacaggtg aacatgatga
     4561 ccttggcaga acacattatt gaagccacac ctgaccgaat caagcaggag aattttgtgc
     4621 ccatggagtc ctcaggattg gaaagaacag accctgccac cattagcagt acaatgagct
     4681 ggctggccag ttatctagcg gatgctgact gccttcccag tgctgctcag atccgaagtg
     4741 catataacga gcctctaacc ccttcttcta ataccagctt gagccctgtt ggctctcccg
     4801 tcagtgaaat cgctttcgag aaacctaacc ttccctccgc cgcggattgg tcagaattcc
     4861 tgagtgcatc taccagtgag aaggtagaga atgagtttgc tcagctcact ctgtctgatc
     4921 atgaacagag agaactctat gaggctgcca ggcttgtcca gacagctttc cggaaataca
     4981 agggccgacc cttgcgggaa cagcaagaag tagctgctgc tgttattcag cgttgttaca
     5041 gaaaatataa acagtacgca ctttataaaa agatgacaca ggctgccatc cttatccaga
     5101 gcaaattccg aagttactat gaacaaaaaa aattccagca gagccgacgg gctgctgtgc
     5161 tcatccaaaa gtactaccga agttataaga aatgtggcaa aagacggcag gctcgccgga
     5221 cggctgtgat tgtacaacag aaactcagga gcagtttgct aaccaaaaag caggatcaag
     5281 ctgctcgaaa aataatgagg tttcttcgcc gctgtcgcca cagccccctg gtggaccata
     5341 ggctgtacaa aaggagtgaa agaattgaaa aaggccaagg aacttgagga tccggggttg
     5401 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     5461 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     5521 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     5581 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     5641 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     5701 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     5761 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     5821 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     5881 cgaatcaccg acctctctcc ccagcaattc accatgaccg agtacaagcc cacggtgcgc
     5941 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6001 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6061 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6121 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6181 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6241 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6301 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6361 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6421 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6481 acctggtgca tgacccgcaa gcccggtgcc gaaggtagag gttctctcct cacttgtggt
     6541 gatgttgaag aaaaccctgg tccaatgtct agactggaca agagcaaagt cataaacgga
     6601 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     6661 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     6721 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     6781 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     6841 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     6901 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     6961 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     7021 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     7081 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     7141 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     7201 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     7261 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag
     7321 gatcgatcca agatatcgta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7381 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7441 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7501 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7561 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7621 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7681 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7741 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7801 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7861 gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg cgtccggact agaggtacct
     7921 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7981 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     8041 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8101 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8161 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8221 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8281 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8341 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8401 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     8461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8521 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8581 gaggcctttc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8641 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8701 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8761 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8821 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8881 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8941 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9001 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9061 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9121 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9181 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9241 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9301 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9361 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9421 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9481 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9541 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10141 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10201 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10261 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10321 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10381 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10441 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10501 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10561 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10621 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    10681 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    10741 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
    10801 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
    10861 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
    10921 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
    10981 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    11041 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    11101 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    11161 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    11221 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    11281 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    11341 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    11401 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    11461 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    11521 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    11581 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    11641 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
    11701 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    11761 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    11821 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    11881 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    11941 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    12001 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    12061 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    12121 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    12181 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    12241 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    12301 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    12361 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    12421 gtttcagacc cacctcccaa ccccgagggg ac
//
