LOCUS       sequence-470563-        7379 bp    DNA     circular SYN 06-JUN-2025
DEFINITION  Express HiBiT-IFIH1 in Mammalian Cells under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7379)
  TITLE     Promega Early Access Collection
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HiBiT-IFIH1 Fusion Vector
FEATURES             Location/Qualifiers
     source          1..7379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4322..4340)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4377..4498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4388..4407)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4442..4461
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4743..5100
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4743..4934
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4766..4786)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4951..5086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5115..5162
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5194..5988
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5248..5267)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5858..5877
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6052..6100
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(6339..6927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6419..6438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    7043..7326
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtg agcggctggc
     1081 ggctgttcaa gaagattagc gggagctccg gtggctcgag cggtgcgatc gccatgtcga
     1141 atgggtattc cacagacgag aatttccgct atctcatctc gtgcttcagg gccagggtga
     1201 aaatgtacat ccaggtggag cctgtgctgg actacctgac ctttctgcct gcagaggtga
     1261 aggagcagat tcagaggaca gtcgccacct ccgggaacat gcaggcagtt gaactgctgc
     1321 tgagcacctt ggagaaggga gtctggcacc ttggttggac tcgggaattc gtggaggccc
     1381 tccggagaac cggcagccct ctggccgccc gctacatgaa ccctgagctc acggacttgc
     1441 cctctccatc gtttgagaac gctcatgatg aatatctcca actgctgaac ctccttcagc
     1501 ccactctggt ggacaagctt ctagttagag acgtcttgga taagtgcatg gaggaggaac
     1561 tgttgacaat tgaagacaga aaccggattg ctgctgcaga aaacaatgga aatgaatcag
     1621 gtgtaagaga gctactaaaa aggattgtgc agaaagaaaa ctggttctct gcatttctga
     1681 atgttcttcg tcaaacagga aacaatgaac ttgtccaaga gttaacaggc tctgattgct
     1741 cagaaagcaa tgcagagatt gagaatttat cacaagttga tggtcctcaa gtggaagagc
     1801 aacttctttc aaccacagtt cagccaaatc tggagaagga ggtctggggc atggagaata
     1861 actcatcaga atcatctttt gcagattctt ctgtagtttc agaatcagac acaagtttgg
     1921 cagaaggaag tgtcagctgc ttagatgaaa gtcttggaca taacagcaac atgggcagtg
     1981 attcaggcac catgggaagt gattcagatg aagagaatgt ggcagcaaga gcatccccgg
     2041 agccagaact ccagctcagg ccttaccaaa tggaagttgc ccagccagcc ttggaaggga
     2101 agaatatcat catctgcctc cctacaggga gtggaaaaac cagagtggct gtttacattg
     2161 ccaaggatca cttagacaag aagaaaaaag catctgagcc tggaaaagtt atagttcttg
     2221 tcaataaggt actgctagtt gaacagctct tccgcaagga gttccaacca tttttgaaga
     2281 aatggtatcg tgttattgga ttaagtggtg atacccaact gaaaatatca tttccagaag
     2341 ttgtcaagtc ctgtgatatt attatcagta cagctcaaat ccttgaaaac tccctcttaa
     2401 acttggaaaa tggagaagat gctggtgttc aattgtcaga cttttccctc attatcattg
     2461 atgaatgtca tcacaccaac aaagaagcag tgtataataa catcatgagg cattatttga
     2521 tgcagaagtt gaaaaacaat agactcaaga aagaaaacaa accagtgatt ccccttcctc
     2581 agatactggg actaacagct tcacctggtg ttggaggggc cacgaagcaa gccaaagctg
     2641 aagaacacat tttaaaacta tgtgccaatc ttgatgcatt tactattaaa actgttaaag
     2701 aaaaccttga tcaactgaaa aaccaaatac aggagccatg caagaagttt gccattgcag
     2761 atgcaaccag agaagatcca tttaaagaga aacttctaga aataatgaca aggattcaaa
     2821 cttattgtca aatgagtcca atgtcagatt ttggaactca accctatgaa caatgggcca
     2881 ttcaaatgga aaaaaaagct gcaaaagaag gaaatcgcaa agaacgtgtt tgtgcagaac
     2941 atttgaggaa gtacaatgag gccctacaaa ttaatgacac aattcgaatg atagatgcgt
     3001 atactcatct tgaaactttc tataatgaag agaaagataa gaagtttgca gtcatagaag
     3061 atgatagtga tgagggtggt gatgatgagt attgtgatgg tgatgaagat gaggatgatt
     3121 taaagaaacc tttgaaactg gatgaaacag atagatttct catgacttta ttttttgaaa
     3181 acaataaaat gttgaaaagg ctggctgaaa acccagaata tgaaaatgaa aagctgacca
     3241 aattaagaaa taccataatg gagcaatata ctaggactga ggaatcagca cgaggaataa
     3301 tctttacaaa aacacgacag agtgcatatg cgctttccca gtggattact gaaaatgaaa
     3361 aatttgctga agtaggagtc aaagcccacc atctgattgg agctggacac agcagtgagt
     3421 tcaaacccat gacacagaat gaacaaaaag aagtcattag taaatttcgc actggaaaaa
     3481 taaatctgct tatcgctacc acagtggcag aagaaggtct ggatattaaa gaatgtaaca
     3541 ttgttatccg ttatggtctc gtcaccaatg aaatagccat ggtccaggcc cgtggtcgag
     3601 ccagagctga tgagagcacc tacgtcctgg ttgctcacag tggttcagga gttatcgaac
     3661 atgagacagt taatgatttc cgagagaaga tgatgtataa agctatacat tgtgttcaaa
     3721 atatgaaacc agaggagtat gctcataaga ttttggaatt acagatgcaa agtataatgg
     3781 aaaagaaaat gaaaaccaag agaaatattg ccaagcatta caagaataac ccatcactaa
     3841 taactttcct ttgcaaaaac tgcagtgtgc tagcctgttc tggggaagat atccatgtaa
     3901 ttgagaaaat gcatcacgtc aatatgaccc cagaattcaa ggaactttac attgtaagag
     3961 aaaacaaagc actgcaaaag aagtgtgccg actatcaaat aaatggtgaa atcatctgca
     4021 aatgtggcca ggcttgggga acaatgatgg tgcacaaagg cttagatttg ccttgtctca
     4081 aaataaggaa ttttgtagtg gttttcaaaa ataattcaac aaagaaacaa tacaaaaagt
     4141 gggtagaatt acctatcaca tttcccaatc ttgactattc agaatgctgt ttatttagtg
     4201 atgaggatgt ttaaacgaat tcgggctcgg tacccgggga tcctctagag tcgacctgca
     4261 ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     4321 accgctgagc aataactagc ataacccctt ggggcggccg cttcgagcag acatgataag
     4381 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4441 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4501 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     4561 aagcaagtaa aacctctaca aatgtggtaa aatcgaattt taacaaaata ttaacgctta
     4621 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4681 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     4741 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4801 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4861 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4921 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4981 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5041 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5101 aagcttaatt aactgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     5161 acaaggtgag gaactaaacc caggaggcag atcatgattg aacaagatgg attgcacgca
     5221 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5281 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5341 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5401 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5461 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5521 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5581 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5641 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5701 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5761 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5821 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5881 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5941 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     6001 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata
     6061 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc
     6121 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     6181 cagcaccgtt tctgcggact ggctttctac ccggtatcag ctcactcaaa ggcggtaata
     6241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6901 attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac
     6961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7021 ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct
     7081 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
     7141 gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag
     7201 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
     7261 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc
     7321 catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatct
//
