LOCUS       sequence-470938-        5330 bp    DNA     circular SYN 06-MAY-2025
DEFINITION  Express SLC35A2-HiBiT in Mammalian Cells under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5330)
  TITLE     Promega Early Access Collection
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SLC35A2-HiBiT Fusion Vector
FEATURES             Location/Qualifiers
     source          1..5330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2409..2427)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    2464..2585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2475..2494)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2529..2548
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2830..3187
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2830..3021
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2853..2873)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3038..3173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3202..3249
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3281..4075
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3335..3354)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3945..3964
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4139..4187
                     /note="synthetic polyadenylation signal"
     rep_origin      4344..4889
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4790..4809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5006..5023
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggct gccgtgggcg
     1081 caggaggaag tacggcagca cctggtccgg gcgcggtgtc tgcaggcgct cttgaacctg
     1141 ggactgcttc agccgcccac cgaagattga agtatatttc tcttgctgtt ctcgtagtgc
     1201 aaaatgcttc cctcatcctt tctataagat acgctcggac gttgccgggc gatcgatttt
     1261 tcgcaacaac cgcagtcgtc atggcagagg tccttaaggg attgacgtgc ctgctccttc
     1321 tctttgcgca aaaacgagga aatgtaaaac acctggttct gtttctgcac gaggcagtgc
     1381 ttgtccaata tgttgacaca ctgaagttgg cggtacccag cctcatttat actttgcaaa
     1441 ataatcttca atatgtcgct atatcaaatt tgccggcagc tacgttccaa gtcacctacc
     1501 aactgaaaat cctgacaacg gcattgttct ccgttcttat gctgaaccgg tccttgtccc
     1561 gactccaatg ggccagtctc cttctcctct ttacaggggt cgccatagtt caagctcaac
     1621 aagcgggagg aggaggtccc cgccctctcg atcagaatcc gggagccggg ctcgcggctg
     1681 tggtggcctc ctgccttagc tctgggttcg cgggtgtata ttttgagaag atcctgaagg
     1741 gttcttctgg tagtgtctgg ttgagaaatc tgcagcttgg cctctttggg acggccctcg
     1801 ggttggtggg tttgtggtgg gcggagggca ccgcagtcgc aactcgcgga ttcttttttg
     1861 gatacacacc agctgtttgg ggtgtcgtat tgaaccaagc atttggcgga ttgctcgtgg
     1921 cggttgttgt gaaatacgcg gacaacatac tgaagggatt cgcgacctcc ttgtccatcg
     1981 tattgtccac cgtcgcctca ataagactct tcgggtttca tgtagatccg ctctttgcct
     2041 tgggtgctgg gcttgtcata ggagcggtct atttgtactc cctgcctcgg ggagcagcga
     2101 aagccatagc ctctgcttcc gcgtctgcct ccggtccgtg cgtccatcag caacctccgg
     2161 gacagccgcc gcctccacag ttgtcctccc accgcggtga cctcataacc gagcccttcc
     2221 tgccgaaatt gctgaccaaa gtgaagggat ccgtttctca aggcagttca ggtggctcga
     2281 gcggtgtgag cggctggcgg ctgttcaaga agattagcta aggccgcgac tctagagtcg
     2341 acctgcaggc atgcaagctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     2401 tgctgccacc gctgagcaat aactagcata accccttggg gcggccgctt cgagcagaca
     2461 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2521 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2581 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     2641 tttttttaag caagtaaaac ctctacaaat gtggtaaaat cgaattttaa caaaatatta
     2701 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2761 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     2821 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     2881 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2941 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3001 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3061 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3121 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3181 ttgcaaaaag cttaattaac tgttgacaat taatcatcgg catagtatat cggcatagta
     3241 taatacgaca aggtgaggaa ctaaacccag gaggcagatc atgattgaac aagatggatt
     3301 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3361 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3421 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     3481 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3541 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3601 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3661 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3721 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     3781 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     3841 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3901 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3961 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4021 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     4081 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa
     4141 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat
     4201 aaggatccgc tcatgcgatg ataagctgtc aaacatgaga attacaactt atatcgtatg
     4261 gggctgactt caggtgctac atttgaagag ataaattgca ctgaaatcta gaaatatttt
     4321 atctgattaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct
     4381 gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct
     4441 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag
     4501 ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc
     4561 cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc
     4621 gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta
     4681 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
     4741 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg ggaaacgcct
     4801 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
     4861 gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc tcacttccct
     4921 gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag ccatttccgc
     4981 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatatcct
     5041 gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac atgaagcact
     5101 tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc tagcgctgat
     5161 gtccggcggt gcttttgccg ttacgcacca ccccgtcatg agattacgcg ttttagcggt
     5221 gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag ccatcgagat
     5281 aaaccgcagt actccggcca cgatgcgtcc ggcgtagagg atcgagatct
//
