LOCUS       sequence-471148-       11137 bp    DNA     circular SYN 31-MAY-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2544
FEATURES             Location/Qualifiers
     source          1..11137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6994..7582
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7047..7067)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7465..7476)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7654..7887
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7965..8086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8002..8021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8056..8075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8126..8261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8281..8300)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8282..8300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8310..8327)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8310..8326)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8319..8341)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8468..8923
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8555..8574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8765..8786
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8949..9053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9054..9122,9123..9914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9272..9291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10085..10673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10574..10593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10827..10844
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10961..10982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10997..11002,11003..11020,11021..11027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11035..11051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11040..11062
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11059..11075
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11059..11075
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11094..11114
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11096..11114
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgttg gagaactaca gaaacctggt
     4141 ctccctagct atgtgttctc atttcaccca agactttttg ccagtgcagg ggatagaaga
     4201 ttcattccac aaacttatac tgagaagata tgagaaatgt gggcatgata atttacaatt
     4261 aaggaaaggc tgtaaaagta tgaacgtgtg taaagtgcag aagggagttt ataatggaat
     4321 taataaatgc ttgtcaaata ctcagagcaa aatatttcaa tgtaatgcac gtgtcaaagt
     4381 ttttagtaaa tttgcaaatt caaacaaaga taagacaaga catactggag agaaacactt
     4441 taaatgtaac gaatgtggca agtcatttca gaagttctca gacctaactc aacataaagg
     4501 aattcatgct ggagagaaac cctacacttg tgaagaacgt ggcaaagact ttggatggta
     4561 cacagacctg aatcaacaca agaaaattca tactggagag aaaccttaca aatgtgaaga
     4621 atgtggcaaa gcctttaata ggtcaacaaa ccttactgca cataagagaa ttcacaacag
     4681 agagaaagct tacacaggtg aagatcgtga cagagccttt ggatggtcca caaacctgaa
     4741 tgaatataag aaaattcata ctggagataa accctacaaa tgtaaagaat gtgggaaagc
     4801 ctttatgcat tcctcacacc tgaataaaca tgagaaaatt catactggag agaaacccta
     4861 caaatgtaag gaatgtggca aagtcatttc ctcatcctca agctttgcta aacataagag
     4921 gattcatact ggcgagaaac cctttaaatg tttagaatgt ggtaaagcct ttaatatttc
     4981 cacaaccctt actaaacata ggagaattca tactggagag aaaccctaca catgtgaagt
     5041 atgtggcaaa gcctttagac agtccgcaaa cctttatgta cataggagaa ttcatactgg
     5101 agagaaaccc tacacatgtg gagaatgtgg caaaaccttt agacagtccg caaaccttta
     5161 cgtacatagg agaattcata ctggagagaa accttacaaa tgcgaagact gtggcaaagc
     5221 ctttggacgg tacacagccc tgaatcaaca caagaaaatt catactggag agaaacctta
     5281 caaatgtgaa gagtgtggca aagcctttaa tagttcaaca aaccttactg cacataagag
     5341 aattcacacc agagagaaac cctacacatg tgaagatcgt ggcagagcct ttggattgtc
     5401 cacaaacctg aatgaatata agaaaattca tactggagat aaaccctaca aatgtaaaga
     5461 atgtgggaaa gcctttatac attccttgca cctgaataaa catgagaaaa ttcatactgg
     5521 aaagaaaccc tacaaatgta agcaatgtgg caaagtcatt acctcatcct caagctttgc
     5581 taaacataag aggattcata ctggcgagaa accctttgaa tgtttagaat gtggtaaagc
     5641 gtttactagt tccacaaccc ttactaaaca taggagaatt catactggag agaaacccta
     5701 cacatgtgaa gtatgtggca aagcctttag acagtccgca atcctttatg tacataggag
     5761 aattcatact ggagagaaac cctacacatg tgaagaatgt ggcaaaacct ttagacagtc
     5821 cgcaaacctt tatgtacata ggagaattca tactggagag aaaccttaca aatgtgaaga
     5881 gtgtggcaaa gcctttggac ggtacacaga cctgaatcaa cacaagaaaa ttcatactgg
     5941 agagaaactt tacaaatgtg aagagtgtgg caaagacttt gtatggtaca cggacctgaa
     6001 tcaacagaag aaaatttaca ctggggagaa accttacaaa tgtgaagagt gtggcaaagc
     6061 ctttgcccca tcaacagacc tgaatcaaca cacgaaaatt cttactggag agcaaagtta
     6121 caaatgtgaa gagtgtggca aagcctttgg atggtccata gccctgaatc aacacaagaa
     6181 aattcatact ggagaaaaac cttacaaatg tgaagagtgt ggcaaagcct ttagtaggtc
     6241 aagaaacctt actacacata ggagagttca cactagggag aaaccctaca aatgtgaaga
     6301 tcgtggcaga tcctttggat ggtccacaaa cctgaacgaa tataagaaaa ttcatactgg
     6361 agataaactc tacaaatgta aagaatgtgg gaaagtgttt aaacagtcct cacacctgaa
     6421 tagacatgag aaaattcata ctggaaagaa accctacaaa tgtaaggaat gtggcaaagt
     6481 cattacgtca tcctcaagct ttgctaaaca taagaggatt catacaggtg agaaaccctt
     6541 taaatgttta gaatgtggta aagcgtttac tagttccaca acccttacta aacataggag
     6601 aattcatact ggagagaaac cctacacatg tgaagaatgt ggcaaagcct ttagacagtc
     6661 agcaatcctt tatgtacata ggagaattca tactggagag aaaccctaca catgtggaga
     6721 atgtggcaaa acctttagac agtctgcaaa tctttatgcg cataagaaaa ttcatactgg
     6781 agagaaaccc tacacgtgtg gagactgtgg caaaaccttt agacagtctg caaatcttta
     6841 tgcacataag aaaattcata ctggagataa aaccatacaa gtgtagtaat gaactagtcc
     6901 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaatgtaa
     6961 attcaaactt attggttaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     7021 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7081 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7141 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7201 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7261 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7321 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7381 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7441 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7501 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7561 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     7621 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7681 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7741 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7801 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7861 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     7921 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     7981 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8041 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8101 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8161 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8221 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8281 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8341 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8401 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8461 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8521 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8581 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8641 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8701 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8761 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8821 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8881 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     8941 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9001 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9061 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10201 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10681 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10741 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10801 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    10921 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    10981 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    11041 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    11101 accctcacta aagggaacaa aagctggagc tgcaagc
//
