LOCUS       sequence-471461-        2560 bp    DNA     circular SYN 05-MAY-2025
DEFINITION  psbB-psbT IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_007_psbB-psbT_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             115..774
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(181..200)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      795..889
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1072..1660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1561..1580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1814..1831
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1855..1912)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    2424..2445
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2448..2491
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2495..2513)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcactcgagt tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca
       61 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
      121 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
      181 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
      241 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
      301 cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg
      361 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
      421 tcatcactct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
      481 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
      541 tttttcgtat cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
      601 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
      661 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
      721 agaatgctta atgaattaca acagtactgc gacgagtggc agggcggggc gtaatttgat
      781 atcgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
      841 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcctcga
      901 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      961 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1201 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1621 atttttgtga tgctcgtcag gggggcggag cctgtggaaa aacgccagca acgcggcctt
     1681 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1741 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1801 aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt
     1861 cggttttaaa gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcagcg
     1921 gccgctacta gtcgcgatga gtggtctcac attaaaaagt aaaatgtatt ttgatttttt
     1981 tgaattaagt ttaattataa atgcatactt tagctacaaa gcaaaagtat ctattgaaat
     2041 taccttaaaa tgaacattgc actaaaacat ttttaaaaat gttttaaaat acttgaagaa
     2101 tttcaagtgt atggctataa aaagaatcat agtaaagtgt taaaggttta aaaagtaaaa
     2161 ttaggtaatt tgctaagcag tcccaaaaag agagtgcttt tttaaactta tacaactttg
     2221 ctgctgtgga gccctccatg tataaataag ttctgtaagt aaaactttta aatacctaaa
     2281 ggacgtcgcc ttacgtgtaa ctaaaccacg taaggcgacc acagtggcag ttgtacttgc
     2341 agagtggttt tatactcggt aaggagacaa acacattctc tgccaactgc cagaaaagaa
     2401 aaacagtacg agaccactca tcgctctaga agcggccgcg aattccagaa atcatcctta
     2461 gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg tgatacgcct atttttatag
     2521 gttaatgtca tgataataat ggtttcttag acgtcaggtg
//
