LOCUS       sequence-471529-        2122 bp    DNA     circular SYN 05-MAY-2025
DEFINITION  3' Operon Connector 1 in the M2 position used to construct operons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_5-6_006_OC3'1
FEATURES             Location/Qualifiers
     source          1..2122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(15..109)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(130..789)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     704..723
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     951..969
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(973..1016)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1019..1040
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1114..1171
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1195..1212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1366..1954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1446..1465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
       61 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      121 gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat
      181 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
      241 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
      301 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa
      361 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      421 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga
      481 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
      541 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
      601 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
      661 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
      721 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
      781 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
      841 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag
      901 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
      961 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
     1021 attcgcggcc gcttctagag cgatgagtgg tctcggctta atactggaga cgagactgag
     1081 accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
     1141 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     1201 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
     1381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1741 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1801 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1981 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2041 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2101 atatgagtaa acttggtctg ac
//
