LOCUS       sequence-471725-       10004 bp    DNA     circular SYN 03-JUN-2025
DEFINITION  Express HA-tagged NUP98::SETBP1 in dual promoter lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10004)
  TITLE     Mullighan lab plasmids 2025
REFERENCE   2  (bases 1 to 10004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CCLMPC_HA-NUP98::SETBP1
FEATURES             Location/Qualifiers
     source          1..10004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1131..1510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1511..1714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1665..1685
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1729..1909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1956..2081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2574..2807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2992..3036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3333..3450
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4053..4077
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4101..4127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6169..6193)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6222..6721
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6257..6280)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6654..6673
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6742..6751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6748..7458
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6893..6911)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7161..7180
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             7580..7813
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7885..8006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7922..7941)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7976..7995
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8046..8181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8201..8220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8202..8220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8230..8247)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8230..8246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8239..8261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8388..8843
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8475..8494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8685..8706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8869..8973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8974..9042,9043..9834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9192..9211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttggcca ttgcatacgt tgtatccata
     1081 tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt
     1141 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1201 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1261 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1321 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1381 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1441 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1501 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     1561 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     1621 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     1681 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg gtctctctgg
     1741 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1801 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1861 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1921 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1981 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2041 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2101 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2161 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2221 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     2281 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     2341 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     2401 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2461 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     2521 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2581 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2641 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2701 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2761 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2821 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2881 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2941 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3001 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3061 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     3121 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3181 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3241 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgataa
     3301 gctaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3361 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3421 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3481 gtttgggaat tagcttgatc gattagtcca atttgttaaa gacaggatat cagtggtcca
     3541 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
     3601 agaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     3661 gtttggcaag ctaggatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     3721 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagttgg aacagcagaa
     3781 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     3841 gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca
     3901 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     3961 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     4021 actcggcgcg atctagatct cgaatcgaat tcacaagttt gtacaaaaaa gcaggctccg
     4081 cggccgcccc cttcaccatg tacccatacg atgttccaga ttacgctttt aacaaatcat
     4141 ttggaacacc ctttgggggt ggcacaggtg gctttggcac aacttcaaca tttggacaga
     4201 atactggctt tggcactact agtggagggg catttggaac atctgcattt ggttctagca
     4261 acaatactgg aggcctcttt ggaaattcac agactaaacc aggaggattg tttggaacca
     4321 gttcatttag ccagccagct acctccacaa gcactggctt tgggtttggt acgtcaacag
     4381 gaacagcaaa taccttgttt ggaactgcaa gcacagggac cagtctcttc tcatcccaaa
     4441 acaatgcctt tgcacaaaat aaaccaactg gctttggcaa ttttggaacc agtactagca
     4501 gtggaggact ctttggaacc acaaatacca cctctaatcc ttttggcagc acatctggct
     4561 ccctctttgg gccaagtagt tttacagctg ctcctactgg gactactatt aaatttaacc
     4621 ctccaactgg tacagatact atggtcaaag ctggagttag cactaacata agtaccaagc
     4681 accagtgtat tactgctatg aaagaatatg aaagcaagtc actagaggaa cttcgtttag
     4741 aggattatca ggctaacagg aagggcccac agaaccaggt gggagcaggt accacaactg
     4801 gcttgtttgg gtcttctcca gccacttcca gcgcaacagg actcttcagc tcctccacca
     4861 ctaattcagg ctttgcatat ggtcagaaca aaactgcctt tggaactagt acaactggat
     4921 ttggaacaaa tccaggtggt ctctttggcc aacagaatca gcagactacc agcctcttca
     4981 gcaaaccatt tggccaggct acaaccaccc agaacactgg cttttccttt ggtaatacca
     5041 gcaccatagg acagccaagc accaacacca tgggattatt tggagtaacc caagcctcac
     5101 agcctggagg tctttttggg acagctacaa acaccagcac tgggacagca tttggaacag
     5161 gaacaggtct ctttgggcag accaatactg gatttggtgc tgttggttcg accctgtttg
     5221 gcaataacaa gcttactaca tttggaagca gcacaaccag tgcaccttca tttggtacaa
     5281 ccagtggcgg gctctttggt tttggcacaa ataccagtgg gaatagtatt tttggaagta
     5341 aaccagcacc tgggactctt ggaactgggc ttggtgcagg atttggaaca gctcttggtg
     5401 ctggacaggc atctttgttt gggaacaacc aacctaagat tggagggcct cttggtacag
     5461 gagcctttgg ggcccctgga tttaatacta cgacagccac tttgggcttt ggagcccccc
     5521 aggccccagt agtcggctcc tccctgaaga agaggttcaa gcggcgggag atcgaagcca
     5581 tccagtgcga agtgcggaag atgtgcaact acaccaagat cctgtccacc aagaagaacc
     5641 tggaccacgt gaacaagatc ctgaaggcca agcggctgca gagacaatca aaaacaggca
     5701 acaacttcgt gaagaagagg cgcgggcgtc ccaggaagca gcccacccag ttcgatgagg
     5761 actccagaga ccaaatgccg gtgctggaaa aatgcatcga cctgcccagc aaaagaggcc
     5821 agaagcccag cctgagcccg ctggtgctgg agcccgccgc cagccaagac accatcatgg
     5881 ccaccatcga ggcggtcatc cacatggccc gggaggcgcc gcccctgccc ccgccaccgc
     5941 cgccgcccct gccgccaccg ccgccaccac ccctgccccc gccaccccct ctacccaaga
     6001 ccccccgagg cggaaagagg aaacacaaac cgcaggcccc cgctcagccc ccacagcagt
     6061 cgcccccgca gcagcccctt ccccaggaag aggaggtgaa agccaaaagg cagaggaagt
     6121 cccgagggag tgagagcgag gtccttccct agaagggtgg gcgcgccgac ccagctttct
     6181 tgtacaaagt ggtgatatcg gtaccgagct cgaattctac cgggtagggg aggcgctttt
     6241 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     6301 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     6361 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     6421 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     6481 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     6541 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     6601 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     6661 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     6721 gaccatctag atccaccgcg ggccaccatg gtgagcaagg gcgaggagga taacatggcc
     6781 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     6841 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     6901 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     6961 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     7021 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     7081 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     7141 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     7201 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     7261 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     7321 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     7381 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     7441 gacgagctgt acaagtagag cggccaactc gagggatccc ccggggtcga ctgatcaaat
     7501 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7561 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7621 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7681 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7741 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7801 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7861 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7921 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7981 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8041 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8101 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8161 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8221 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8281 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8341 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8401 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8461 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8521 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8581 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8641 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8701 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8761 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8821 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8881 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8941 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9001 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9061 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9121 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9181 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9241 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9301 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9361 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9421 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9481 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9541 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9601 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9661 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9721 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9781 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9841 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9901 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9961 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
