LOCUS       sequence-471876-       10711 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Express NanoLuc(R)-CIT Fusion Protein in Mammalian Cells under a CMV
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  TITLE     Promega Early Access Collection
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NanoLuc-CIT Fusion Vector
FEATURES             Location/Qualifiers
     source          1..10711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1065..1577
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(7790..7808)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    7845..7966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7856..7875)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7910..7929
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8211..8568
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8211..8402
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8234..8254)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8419..8554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8583..8630
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8662..9456
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8716..8735)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9326..9345
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9520..9568
                     /note="synthetic polyadenylation signal"
     rep_origin      9725..10270
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     10171..10190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10387..10404
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gttgaagttc aaatatggag
     1621 cgcggaatcc tttggatgct ggtgctgctg aacccattgc cagccgggcc tccaggctga
     1681 atctgttctt ccaggggaaa ccacccttta tgactcaaca gcagatgtct cctctttccc
     1741 gagaagggat attagatgcc ctctttgttc tctttgaaga atgcagtcag cctgctctga
     1801 tgaagattaa gcacgtgagc aactttgtcc ggaagtattc cgacaccata gctgagttac
     1861 aggagctcca gccttcggca aaggacttcg aagtcagaag tcttgtaggt tgtggtcact
     1921 ttgctgaagt gcaggtggta agagagaaag caaccgggga catctatgct atgaaagtga
     1981 tgaagaagaa ggctttattg gcccaggagc aggtttcatt ttttgaggaa gagcggaaca
     2041 tattatctcg aagcacaagc ccgtggatcc cccaattaca gtatgccttt caggacaaaa
     2101 atcaccttta tctggtcatg gaatatcagc ctggagggga cttgctgtca cttttgaata
     2161 gatatgagga ccagttagat gaaaacctga tacagtttta cctagctgag ctgattttgg
     2221 ctgttcacag cgttcatctg atgggatacg tgcatcgaga catcaagcct gagaacattc
     2281 tcgttgaccg cacaggacac atcaagctgg tggattttgg atctgccgcg aaaatgaatt
     2341 caaacaagat ggtgaatgcc aaactcccga ttgggacccc agattacatg gctcctgaag
     2401 tgctgactgt gatgaacggg gatggaaaag gcacctacgg cctggactgt gactggtggt
     2461 cagtgggcgt gattgcctat gagatgattt atgggagatc ccccttcgca gagggaacct
     2521 ctgccagaac cttcaataac attatgaatt tccagcggtt tttgaaattt ccagatgacc
     2581 ccaaagtgag cagtgacttt cttgatctga ttcaaagctt gttgtgcggc cagaaagaga
     2641 gactgaagtt tgaaggtctt tgctgccatc ctttcttctc taaaattgac tggaacaaca
     2701 ttcgtaactc tcctcccccc ttcgttccca ccctcaagtc tgacgatgac acctccaatt
     2761 ttgatgaacc agagaagaat tcgtgggttt catcctctcc gtgccagctg agcccctcag
     2821 gcttctcggg tgaagaactg ccgtttgtgg ggttttcgta cagcaaggca ctggggattc
     2881 ttggtagatc tgagtctgtt gtgtcgggtc tggactcccc tgccaagact agctccatgg
     2941 aaaagaaact tctcatcaaa agcaaagagc tacaagactc tcaggacaag tgtcacaaga
     3001 tggagcagga aatgacccgg ttacatcgga gagtgtcaga ggtggaggct gtgcttagtc
     3061 agaaggaggt ggagctgaag gcctctgaga ctcagagatc cctcctggag caggaccttg
     3121 ctacctacat cacagaatgc agtagcttaa agcgaagttt ggagcaagca cggatggagg
     3181 tgtcccagga ggatgacaaa gcactgcagc ttctccatga tatcagagag cagagccgga
     3241 agctccaaga aatcaaagag caggagtacc aggctcaagt ggaagaaatg aggttgatga
     3301 tgaatcagtt ggaagaggat cttgtctcag caagaagacg gagtgatctc tacgaatctg
     3361 agctgagaga gtctcggctt gctgctgaag aattcaagcg gaaagcgaca gaatgtcagc
     3421 ataaactgtt gaaggctaag gatcaaggga agcctgaagt gggagaatat gcgaaactgg
     3481 agaagatcaa tgctgagcag cagctcaaaa ttcaggagct ccaagagaaa ctggagaagg
     3541 ctgtaaaagc cagcacggag gccaccgagc tgctgcagaa tatccgccag gcaaaggagc
     3601 gagccgagag ggagctggag aagctgcaga accgagagga ttcttctgaa ggcatcagaa
     3661 agaagctggt ggaagctgag gagctcgaag agaaacatcg ggaggcccaa gtctcagccc
     3721 agcacctaga agtgcacctg aaacagaaag agcagcacta tgaggaaaag attaaagtgt
     3781 tggacaatca gataaagaaa gacctggctg acaaggagac actggagaac atgatgcaga
     3841 gacacgagga ggaggcccat gagaagggca aaattctcag cgaacagaag gcgatgatca
     3901 atgctatgga ttccaagatc agatccctgg aacagaggat tgtggaactg tctgaagcca
     3961 ataaacttgc agcaaatagc agtcttttta cccaaaggaa catgaaggcc caagaagaga
     4021 tgatttctga actcaggcaa cagaaatttt acctggagac acaggctggg aagttggagg
     4081 cccagaaccg aaaactggag gagcagctgg agaagatcag ccaccaagac cacagtgaca
     4141 agaatcggct gctggaactg gagacaagat tgcgggaggt cagtctagag cacgaggagc
     4201 agaaactgga gctcaagcgc cagctcacag agctacagct ctccctgcag gagcgcgagt
     4261 cacagttgac agccctgcag gctgcacggg cggccctgga gagccagctt cgccaggcga
     4321 agacagagct ggaagagacc acagcagaag ctgaagagga gatccaggca ctcacggcac
     4381 atagagatga aatccagcgc aaatttgatg ctcttcgtaa cagctgtact gtaatcacag
     4441 acctggagga gcagctaaac cagctgaccg aggacaacgc tgaactcaac aaccaaaact
     4501 tctacttgtc caaacaactc gatgaggctt ctggcgccaa cgacgagatt gtacaactgc
     4561 gaagtgaagt ggaccatctc cgccgggaga tcacggaacg agagatgcag cttaccagcc
     4621 agaagcaaac gatggaggct ctgaagacca cgtgcaccat gctggaggaa caggtcatgg
     4681 atttggaggc cctaaacgat gagctgctag aaaaagagcg gcagtgggag gcctggagga
     4741 gcgtcctggg tgatgagaaa tcccagtttg agtgtcgggt tcgagagctg cagagaatgc
     4801 tggacaccga gaaacagagc agggcgagag ccgatcagcg gatcaccgag tctcgccagg
     4861 tggtggagct ggcagtgaag gagcacaagg ctgagattct cgctctgcag caggctctca
     4921 aagagcagaa gctgaaggcc gagagcctct ctgacaagct caatgacctg gagaagaagc
     4981 atgctatgct tgaaatgaat gcccgaagct tacagcagaa gctggagact gaacgagagc
     5041 tcaaacagag gcttctggaa gagcaagcca aattacagca gcagatggac ctgcagaaaa
     5101 atcacatttt ccgtctgact caaggactgc aagaagctct agatcgggct gatctactga
     5161 agacagaaag aagtgacttg gagtatcagc tggaaaacat tcaggttctc tattctcatg
     5221 aaaaggtgaa aatggaaggc actatttctc aacaaaccaa actcattgat tttctgcaag
     5281 ccaaaatgga ccaacctgct aaaaagaaaa agggtttatt tagtcgacgg aaagaggacc
     5341 ctgctttacc cacacaggtt cctctgcagt acaatgagct gaagctggcc ctggagaagg
     5401 agaaagctcg ctgtgcagag ctagaggaag cccttcagaa gacccgcatc gagctccggt
     5461 ccgcccggga ggaagctgcc caccgcaaag caacggacca cccacaccca tccacgccag
     5521 ccaccgcgag gcagcagatc gccatgtccg ccatcgtgcg gtcgccagag caccagccca
     5581 gtgccatgag cctgctggcc ccgccatcca gccgcagaaa ggagtcttca actccagagg
     5641 aatttagtcg gcgtcttaag gaacgcatgc accacaatat tcctcaccga ttcaacgtag
     5701 gactgaacat gcgagccaca aagtgtgctg tgtgtctgga taccgtgcac tttggacgcc
     5761 aggcatccaa atgtctcgaa tgtcaggtga tgtgtcaccc caagtgctcc acgtgcttgc
     5821 cagccacctg cggcttgcct gctgaatatg ccacacactt caccgaggcc ttctgccgtg
     5881 acaaaatgaa ctccccaggt ctccagacca aggagcccag cagcagcttg cacctggaag
     5941 ggtggatgaa ggtgcccagg aataacaaac gaggacagca aggctgggac aggaagtaca
     6001 ttgtcctgga gggatcaaaa gtcctcattt atgacaatga agccagagaa gctggacaga
     6061 ggccggtgga agaatttgag ctgtgccttc ccgacgggga tgtatctatt catggtgccg
     6121 ttggtgcttc cgaactcgca aatacagcca aagcagatgt cccatacata ctgaagatgg
     6181 aatctcaccc gcacaccacc tgctggcccg ggagaaccct ctacttgcta gctcccagct
     6241 tccctgacaa acagcgctgg gtcaccgcct tagaatcagt tgtcgcaggt gggagagttt
     6301 ctagggaaaa agcagaagct gatgctaaac tgcttggaaa ctccctgctg aaactggaag
     6361 gtgatgaccg tctagacatg aactgcacgc tgcccttcag tgaccaggtg gtgttggtgg
     6421 gcaccgagga agggctctac gccctgaatg tcttgaaaaa ctccctaacc catgtcccag
     6481 gaattggagc agtcttccaa atttatatta tcaaggacct ggagaagcta ctcatgatag
     6541 caggagaaga gcgggcactg tgtcttgtgg acgtgaagaa agtgaaacag tccctggccc
     6601 agtcccacct gcctgcccag cccgacatct cacccaacat ttttgaagct gtcaagggct
     6661 gccacttgtt tggggcaggc aagattgaga acgggctctg catctgtgca gccatgccca
     6721 gcaaagtcgt cattctccgc tacaacgaaa acctcagcaa atactgcatc cggaaagaga
     6781 tagagacctc agagccctgc agctgtatcc acttcaccaa ttacagtatc ctcattggaa
     6841 ccaataaatt ctacgaaatc gacatgaagc agtacacgct cgaggaattc ctggataaga
     6901 atgaccattc cttggcacct gctgtgtttg ccgcctcttc caacagcttc cctgtctcaa
     6961 tcgtgcaggt gaacagcgca gggcagcgag aggagtactt gctgtgtttc cacgaatttg
     7021 gagtgttcgt ggattcttac ggaagacgta gccgcacaga cgatctcaag tggagtcgct
     7081 tacctttggc ctttgcctac agagaaccct atctgtttgt gacccacttc aactcactcg
     7141 aagtaattga gatccaggca cgctcctcag cagggacccc tgcccgagcg tacctggaca
     7201 tcccgaaccc gcgctacctg ggccctgcca tttcctcagg agcgatttac ttggcgtcct
     7261 cataccagga taaattaagg gtcatttgct gcaagggaaa cctcgtgaag gagtccggca
     7321 ctgaacacca ccggggcccg tccacctccc gcagcagccc caacaagcga ggcccaccca
     7381 cgtacaacga gcacatcacc aagcgcgtgg cctccagccc agcgccgccc gaaggcccca
     7441 gccacccgcg agagccaagc acaccccacc gctaccgcga ggggcggacc gagctgcgca
     7501 gggacaagtc tcctggccgc cccctggagc gagagaagtc ccccggccgg atgctcagca
     7561 cgcggagaga gcggtccccc gggaggctgt ttgaagacag cagcaggggc cggctgcctg
     7621 cgggagccgt gaggaccccg ctgtcccagg tgaacaaggt ctgggaccag tcttcagttt
     7681 aaacgaattc gggctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     7741 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     7801 taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat acattgatga
     7861 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     7921 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     7981 cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa
     8041 cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca atttcctgat
     8101 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca
     8161 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     8221 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     8281 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8341 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     8401 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8461 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8521 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaattaa
     8581 ctgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     8641 actaaaccca ggaggcagat catgattgaa caagatggat tgcacgcagg ttctccggcc
     8701 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     8761 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     8821 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     8881 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     8941 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     9001 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     9061 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     9121 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     9181 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     9241 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     9301 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     9361 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     9421 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     9481 ccgaccaagc gacgcccaac ctgccatcac gatggccgca ataaaatatc tttattttca
     9541 ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga taaggatccg ctcatgcgat
     9601 gataagctgt caaacatgag aattacaact tatatcgtat ggggctgact tcaggtgcta
     9661 catttgaaga gataaattgc actgaaatct agaaatattt tatctgatta ataagatgat
     9721 cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct
     9781 tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc
     9841 ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact
     9901 tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt
     9961 ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg
    10021 gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg
    10081 cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg
    10141 aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt atagtcctgt
    10201 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag
    10261 cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca
    10321 tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc
    10381 gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga
    10441 cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag
    10501 tgccaacata gtaagccagt atacactccg ctagcgctga tgtccggcgg tgcttttgcc
    10561 gttacgcacc accccgtcat gagattacgc gttttagcgg tgcgtacaat taagggatta
    10621 tggtaaatcc acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc
    10681 acgatgcgtc cggcgtagag gatcgagatc t
//
