LOCUS       sequence-472102-       10368 bp    DNA     circular SYN 07-MAY-2025
DEFINITION  To express tagRFP657-2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10368)
  TITLE     Manel lab plasmids 2025
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTRIP-SFFV-tagRFP657-2A
FEATURES             Location/Qualifiers
     source          1..10368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKV
                     VEGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTALALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             4772..4952
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6480..6499)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6713..6732)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6832..6854
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6892..6910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6978..7082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7083..7151,7152..7943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7301..7320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8114..8702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8603..8622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8856..8873
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8943..8963)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8948..9277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9128..9263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
     3781 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 cagagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
     3901 agcttcatgt acggcagtca caccttcatc aaccacaccc agggcatccc cgacttctgg
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggcccaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag aaccgcgctg
     4201 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc
     4261 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc cactagtgtc
     4501 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     4561 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     4621 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4681 aaaagggggg actggaaggg ctaattcact cccaacgaag acaaaatcgt cgagagatgc
     4741 tgcatataag cagctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     4801 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4861 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4921 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     4981 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attataatag
     5041 atttagcagg aattgaacta ggagtggagc acacaggcaa agctgcagaa gtacttggaa
     5101 gaagccacca gagatactca cgattctgca catacctggc taatcccaga tcctaaggat
     5161 tacattaagt ttactaacat ttatataatg atttatagtt taaagtataa acttatctaa
     5221 tttactattc tgacagatat taattaatcc tcaaatatca taagagatga ttactattat
     5281 ccccatttaa cacaagagga aactgagagg gaaagatgtt gaagtaattt tcccacaatt
     5341 acagcatccg ttagttacga ctctatgatc ttctgacaca aattccattt actcctcacc
     5401 ctatgactca gtcgaatata tcaaagttat ggacattatg ctaagtaaca aattaccctt
     5461 ttatatagta aatactgagt agattgagag aagaaattgt ttgcaaacct gaatagcttc
     5521 aagaagaaga gaagtgagga taagaataac agttgtcatt taacaagttt taacaagtaa
     5581 cttggttaga aagggattca aatgcataaa gcaagggata aatttttctg gcaacaagac
     5641 tatacaatat aaccttaaat atgacttcaa ataattgttg gaacttgata aaactaatta
     5701 aatattattg aagattatca atattataaa tgtaatttac ttttaaaaag ggaacataga
     5761 aatgtgtatc attagagtag aaaacaatcc ttattatcac aatttgtcaa aacaagtttg
     5821 ttattaacac aagtagaata ctgcattcaa ttaagttgac tgcagatttt gtgttttgtt
     5881 aaaattagaa agagataaca acaatttgaa ttattgaaag taacatgtaa atagttctac
     5941 atacgttctt ttgacatctt gttcaatcat tgatcgaagt tctttatctt ggaagaattt
     6001 gttccaaaga ctctgaaata aggaaaacaa tctattatat agtctcacac ctttgtttta
     6061 cttttagtga tttcaattta ataatgtaaa tggttaaaat ttattcttct ctgagatcat
     6121 ttcacattgc agatagaaaa cctgagactg gggtaatttt tattaaaatc taatttaatc
     6181 tcagaaacac atctttattc taacatcaat ttttccagtt tgatattatc atataaagtc
     6241 agccttcctc atctgcaggt tccacaacaa aaatccaacc aactgtggat caaaaatatt
     6301 gggaaaaaat taaaaatagc aatacaacaa taaaaaaata caaatcagaa aaacagcaca
     6361 gtataacaac tttatttagc atttacaatc tattaggtat tataagtaat ctagccagat
     6421 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     6481 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     6541 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     6601 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     6661 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
     6721 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     6781 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     6841 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     6901 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8221 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8761 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8821 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8881 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8941 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9001 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9061 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9121 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9181 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9241 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     9301 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     9361 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     9421 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     9481 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     9541 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     9601 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     9661 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     9721 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     9781 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     9841 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     9901 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     9961 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
    10021 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
    10081 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
    10141 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
    10201 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
    10261 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
    10321 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtg
//
