LOCUS       sequence-472164-        8460 bp    DNA     circular SYN 08-MAY-2025
DEFINITION  Expresses Myc-Sr62TK in planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Chen R, Chen J, Outram MA, Powell OR, Arndell T, Gajendiran K, Wang 
            YL, Ayliffe MA, Blundell C, Figueroa M, Sperschneider J, Vanhercke 
            T, Tang D, Xu Y, Zhong G, Gardener C, Yu G, Gourdoupis S, Jaremko Ł,
            Matny O, Steffenson BJ, Boshoff WHP, Meyer WB, Arold ST, Dodds PN, 
            Wulff BBH
  TITLE     A wheat tandem kinase sensor activates an NLR helper to trigger 
            immunity
  JOURNAL   bioRxiv 2024.08.30.610287
REFERENCE   2  (bases 1 to 8460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.08.30.610287"
COMMENT     Sequence Label: pICH47822_Myc-Sr62TK
FEATURES             Location/Qualifiers
     source          1..8460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             271..1419
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    1575..1599
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2979
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2922..2942
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3074..3163
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             3104..3193
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     terminator      5474..5726
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5837..5861
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      5953..6584
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6671..6775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6776..6844,6845..7636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6994..7013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7807..8395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8296..8315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca ggccgaggcc
       61 cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat tcggaatacc
      121 aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat
      181 tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg
      241 tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac
      301 aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc
      361 accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct
      421 acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg
      481 gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg caggtttggc gaagtcgatg
      541 accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg
      601 gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc
      661 aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg
      721 ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag
      781 gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc
      841 gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag
      901 cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc
      961 tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg
     1021 acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac
     1081 cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag
     1141 gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc
     1201 aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac
     1261 ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag
     1321 tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac
     1381 gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg
     1441 gctgggggtt cagcagccag cgcctgatct ggggaaccct gtggttggca tgcacataca
     1501 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg
     1561 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaaccac
     1621 ttcgtgcaga agacaagtaa ggaggaattc caatcccaca aaaatctgag cttaacagca
     1681 cagttgctcc tctcagagca gaatcgggta ttcaacaccc tcatatcaac tactacgttg
     1741 tgtataacgg tccacatgcc ggtatatacg atgactgggg ttgtacaaag gcggcaacaa
     1801 acggcgttcc cggagttgca cacaagaaat ttgccactat tacagaggca agagcagcag
     1861 ctgacgcgta cacaacaagt cagcaaacag acaggttgaa cttcatcccc aaaggagaag
     1921 ctcaactcaa gcccaagagc tttgctaagg ccctaacaag cccaccaaag caaaaagccc
     1981 actggctcac gctaggaacc aaaaggccca gcagtgatcc agccccaaaa gagatctcct
     2041 ttgccccgga gattacaatg gacgatttcc tctatcttta cgatctagga aggaagttcg
     2101 aaggtgaagg tgacgacact atgttcacca ctgataatga gaaggttagc ctcttcaatt
     2161 tcagaaagaa tgctgaccca cagatggtta gagaggccta cgcagcaagt ctcatcaaga
     2221 cgatctaccc gagtaacaat ctccaggaga tcaaatacct tcccaagaag gttaaagatg
     2281 cagtcaaaag attcaggact aattgcatca agaacacaga gaaagacata tttctcaaga
     2341 tcagaagtac tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa
     2401 tagagattgg agtctctaaa aaggtagttc ctactgaatc taaggccatg catggagtct
     2461 aagattcaaa tcgaggatct aacagaactc gccgtcaaga ctggcgaaca gttcatacag
     2521 agtcttttac gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact
     2581 ctggtctact ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact
     2641 tttcaacaaa ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     2701 ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
     2761 ggaaaggcta tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc
     2821 acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga
     2881 tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     2941 tcctctatat aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat
     3001 ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt
     3061 atcgataccc atggaacaaa agttgatctc tgaagaggac cttgagcaga aattgatctc
     3121 tgaggaagat cttgagcaga agcttatctc agaggaggat ttggagcaaa aacttatttc
     3181 tgaagaggac ctaatgtacg cgtcccgggg cggtaccatg gacggatacg agttcgagag
     3241 ggcggagata gatgcactgg agcgcgtcgt acgggatcca agagaaaagc caataagcct
     3301 gtcgttttgg cttctcaggc gcataacgaa tgatttctct aacgcatctg aaattggtcg
     3361 aggtggattt tcgaacgttt acctgggggt gcttccaagt gggttgcgta ttgctgtcaa
     3421 aaagctttac caacttactg cattggctaa tgaatttgaa gatgaagttc ttaccacaat
     3481 gagggttgct cacaaaaaca tagtgcgact cataggctac tgtcgtcaca cgcaatctga
     3541 agtcttcgaa ttaaaaggag aatctatttt cgcacaggtc agagaaaggt tgatctgtac
     3601 agagtatgtg cctaatggaa ctcttgatgg acatatcact gataagtttc atggaggact
     3661 tgattgggac cagcgttatc aagttctcaa aggaatttgt cacggtttgc attatctgca
     3721 cgatgaaata cgcgttatcc acagagatat caaaacagaa aatatattgc tagatgataa
     3781 cggcgtgcca aaagtttttg actttggttt ttcgaagtgc cttcctgatg gagaatcttt
     3841 aattaaagat gaagcaaccc agggaaccct agggtatttg gcgccggagt cagttgataa
     3901 gcaagaatat tcctttaagt ccgacatata cagctttggt gttgtgatca tacaattatt
     3961 gactggatcg tggatcagta agaaccgaaa tgttgagcta gaacttaagg agttgagaaa
     4021 aaggttggta agagaaggag catttccatc atgggaaaac aaataccacc aagttagaac
     4081 atgtctggag attgtgtata actgcatgga catcgaccca gataaaaggc cgactgcatt
     4141 ggaaattatc caacggcttg atgaaaccga aagcgcgaac tattctgtta gcagtggcca
     4201 tgcaacacca ctttggcagt caggagatga agaatccgac tcagcggaag tggatgcttt
     4261 tgagccagag agaacaccat caaaagacat tctttcagat gatgaggaac ccgcatcagc
     4321 agacgcaaat ggagaaacga gcacacaaaa acataatatg cctgatcttg taagtaaaca
     4381 gtcagtgtcg gcggaactgt cctcgctaga tttccttgag aaaatcacag atggtttttc
     4441 atacgagcaa atactcggga aggaaagtct ggacgcagat gttcataaag catttcttta
     4501 tgagggcagc atttcaggtc cagcaaaggt agttgtgaag aggttaattg gagtagcaat
     4561 tccagttgcg aagtttaaga gggatgcaga acagctgatg agcctggatc acaagaatat
     4621 agtaaagctc cttggctact gccacgacga atctagaggg cataagctgg tacagtttaa
     4681 agcaaaacca ccgcaagatt ttaaaggagc cgagcaactt ctctgctatg aatatatgca
     4741 caatggaagc ctccgtgagt atcttactgg acaaggatct cgtgaagttg attggcacat
     4801 gtgctacaaa ttgatcaaag ggacctgcga aggtttacgc tacctccatg agggttgtgg
     4861 agatcgtcca attcttcatt tgaatctaaa cccgtcaaat atactgctgg acgaaaacaa
     4921 cataccacgc atcacgggtt ttgatttttc gaagctcatt ggtgaaaaga acacaaaatc
     4981 cgtggttctt aagaagaatg gaccactagg ttacctgcca ccggacttcc tgtactcaaa
     5041 gggtactgac ctcaagtatc tacctacggt ggacatatac agcttgggtc ttataatttt
     5101 agagattgca acacgtcaag agatcaaggg agaccatgga ataattatta ggactgtcga
     5161 gacaaactgg aggcaagata cacaaatagc aacactgtat ggttcactag aagataaatt
     5221 acgggagcaa gtaaaaatgt gcattgacat tggcctagac tgtgtcaagt caaaccctaa
     5281 aaagagacct acagctgggg ccatcatgct ctggcttgac aaagggagca aaccgggagt
     5341 tgccagacct ccactccgcg ataatgtcac ccatgcagat gatcacatcc aagaaaagga
     5401 gaagcagacg ccattgagga gatttttcag aagaaagtaa gtcgacctgc agagctttcg
     5461 tgcttgtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5521 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     5581 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     5641 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     5701 cgcggtgtca tctatgttac tagatcgacg ctgagctcga attctagttt gtcttcacag
     5761 agtggggccc actgcatcca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     5821 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     5881 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtcagatcag
     5941 gatctccttt gcgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta
     6001 tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg
     6061 gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga
     6121 cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg
     6181 gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag
     6241 tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag
     6301 gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga
     6361 cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat
     6421 ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa
     6481 accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac
     6541 gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat
     6601 ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagcgctgg
     6661 ccaattcgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6721 gctcatgaga caataacctt gataaatgct tcaataatat tgaaaaagga agagtatgag
     6781 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6841 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6901 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6961 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7021 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7081 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7141 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7201 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7261 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7321 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7381 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7441 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gttctcgcgg
     7501 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7561 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7621 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7681 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7741 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7801 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7861 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7921 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7981 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8041 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8101 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8161 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8221 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8281 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8341 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8401 cagcaacgcg gcctttttac ggttcctggc agatcctaga tgtggcgcaa cgatgccggc
//
