LOCUS       sequence-472390-       10343 bp    DNA     circular SYN 13-MAY-2025
DEFINITION  To express GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10343)
  TITLE     Manel lab plasmids 2025
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTRIP-SFFV
FEATURES             Location/Qualifiers
     source          1..10343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3736..3745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3742..3744,3745..3747,3748..4461)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3742..3744,3745..3747,3748..4458)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3787..3808)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4048..4067)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4395..4416
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4747..4927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6455..6474)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6688..6707)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6807..6829
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6867..6885)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6953..7057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7058..7126,7127..7918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7276..7295)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8089..8677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8578..8597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8831..8848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8918..8938)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8923..9252
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9103..9238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3781 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3841 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3901 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3961 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4021 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4081 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4141 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4201 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4261 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4321 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4381 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4441 atggacgagc tgtacaagta aagcggccgc gactctagct cgagacctag aaaaacatgg
     4501 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     4561 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     4621 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4681 tcactcccaa cgaagacaaa atcgtcgaga gatgctgcat ataagcagct gctttttgct
     4741 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4801 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4861 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4921 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     4981 atatcagaga gtgagaggcc ttgacattat aatagattta gcaggaattg aactaggagt
     5041 ggagcacaca ggcaaagctg cagaagtact tggaagaagc caccagagat actcacgatt
     5101 ctgcacatac ctggctaatc ccagatccta aggattacat taagtttact aacatttata
     5161 taatgattta tagtttaaag tataaactta tctaatttac tattctgaca gatattaatt
     5221 aatcctcaaa tatcataaga gatgattact attatcccca tttaacacaa gaggaaactg
     5281 agagggaaag atgttgaagt aattttccca caattacagc atccgttagt tacgactcta
     5341 tgatcttctg acacaaattc catttactcc tcaccctatg actcagtcga atatatcaaa
     5401 gttatggaca ttatgctaag taacaaatta cccttttata tagtaaatac tgagtagatt
     5461 gagagaagaa attgtttgca aacctgaata gcttcaagaa gaagagaagt gaggataaga
     5521 ataacagttg tcatttaaca agttttaaca agtaacttgg ttagaaaggg attcaaatgc
     5581 ataaagcaag ggataaattt ttctggcaac aagactatac aatataacct taaatatgac
     5641 ttcaaataat tgttggaact tgataaaact aattaaatat tattgaagat tatcaatatt
     5701 ataaatgtaa tttactttta aaaagggaac atagaaatgt gtatcattag agtagaaaac
     5761 aatccttatt atcacaattt gtcaaaacaa gtttgttatt aacacaagta gaatactgca
     5821 ttcaattaag ttgactgcag attttgtgtt ttgttaaaat tagaaagaga taacaacaat
     5881 ttgaattatt gaaagtaaca tgtaaatagt tctacatacg ttcttttgac atcttgttca
     5941 atcattgatc gaagttcttt atcttggaag aatttgttcc aaagactctg aaataaggaa
     6001 aacaatctat tatatagtct cacacctttg ttttactttt agtgatttca atttaataat
     6061 gtaaatggtt aaaatttatt cttctctgag atcatttcac attgcagata gaaaacctga
     6121 gactggggta atttttatta aaatctaatt taatctcaga aacacatctt tattctaaca
     6181 tcaatttttc cagtttgata ttatcatata aagtcagcct tcctcatctg caggttccac
     6241 aacaaaaatc caaccaactg tggatcaaaa atattgggaa aaaattaaaa atagcaatac
     6301 aacaataaaa aaatacaaat cagaaaaaca gcacagtata acaactttat ttagcattta
     6361 caatctatta ggtattataa gtaatctagc cagatcctct acgccggacg catcgtggcc
     6421 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     6481 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     6541 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     6601 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     6661 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6721 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     6781 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     6841 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6901 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6961 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7021 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7081 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7141 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7201 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7261 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7321 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7381 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7441 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7501 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7561 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7621 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7681 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7741 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7801 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7861 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7921 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7981 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8041 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8101 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8161 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8221 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8281 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8341 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8401 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8461 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8521 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8581 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8641 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8701 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8761 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8821 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8881 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     8941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9001 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9061 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9121 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9181 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9241 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     9301 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     9361 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     9421 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     9481 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     9541 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     9601 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     9661 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     9721 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     9781 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     9841 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     9901 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     9961 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
    10021 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
    10081 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
    10141 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
    10201 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
    10261 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
    10321 aggaacctta cttctgtggt gtg
//
