LOCUS       sequence-472461-        6191 bp    DNA     circular SYN 13-MAY-2025
DEFINITION  Express NanoLuc(R)-CCDC6 Fusion Protein in Mammalian Cells under a 
            CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6191)
  TITLE     Promega Early Access Collection
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NanoLuc-CCDC6 Vector
FEATURES             Location/Qualifiers
     source          1..6191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(351..863)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(876..895)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(877..895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(937..956)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          939..1071
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(1207..1410)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1236..1256)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1411..1790
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    1981..2264
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     rep_origin      2380..2968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2869..2888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3207..3255
                     /note="synthetic polyadenylation signal"
     CDS             complement(3319..4113)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3430..3449)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4040..4059
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4145..4192)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4207..4564)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4221..4356
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4373..4564
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4521..4541
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4809..4930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4846..4865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4900..4919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4967..4985
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tcttcttcct gctcagccct ggcttggatg gtcacgctgg ctttgcgcag gtcgcggttc
       61 tcctcctgca gtgccttgca cttcagtttg taggtctcca gctctatctt cagcaccttg
      121 ttctcttgct gcagcgaggc caggcggttg gtgagctcct ccaggcggaa cggcgagatg
      181 acaatgcccc ccgacttccc accgccgccg cctcccccgc cgccaccgcc gccgcccgag
      241 gtcgacgagc aggacgactg catggcggcc gagctgctgc tgttgccccc cgccccgtcc
      301 gtgtcgctct cgctggcgct gtccgccatg gcgatcgcgc cgctcgagcc cgccagaatg
      361 cgttcgcaca gccgccagcc ggtcactccg ttgatggtta ctcggaacag cagggagccg
      421 tcggggttga tcaggcgctc gtcgataatt ttgttgccgt tccacagggt ccctgttaca
      481 gtgatctttt tgccgtcgaa cacggcgatg ccttcatacg gccgtccgaa atagtcgatc
      541 atgttcggcg taaccccgtc gattaccagt gtgccatagt gcaggatcac cttaaagtga
      601 tgatcatcca cagggtacac caccttaaaa attttttcga tctggcccat ttggtcgccg
      661 ctcagacctt catacgggat gatgacatgg atgtcgatct tcagcccatt ttcaccgctc
      721 aggacaatcc tttggatcgg agttacggac accccgagat tctgaaacaa actggacaca
      781 cctccctgtt caaggacttg gtccaggttg tagccggctg tctgtcgcca gtccccaacg
      841 aaatcttcga gtgtgaagac catggtgagc gctagcccta tagtgagtcg tattaatact
      901 ctagccttaa gagctgtaat tgaactggga gtggacacct gtggagagaa aggcaaagtg
      961 gatgtcagta agaccaatag gtgcctatca gaaacgcaag agtcttctct gtctcgacaa
     1021 gcccagtttc tattggtctc cttaaacctg tcttgtaacc ttgatactta cctgcccagt
     1081 gcctcacgac caacttctgc agcttaagtt cgagactgtt gtgtcagaag cactgactgc
     1141 gttagcaatt taactgtgat aaactaccgc aataaagctt ctagtgatct gacggttcac
     1201 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     1261 cggggcgggg ttattacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     1321 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     1381 cccattggtg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     1441 gtactgccaa gtaggaaagt cccgtaaggt catgtactgg gcataatgcc aggcgggcca
     1501 tttaccgtca ttgacgtcaa tagggggcgg acttggcata tgatacactt gatgtactgc
     1561 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     1621 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     1681 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     1741 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgcc aacatggcgg
     1801 tcatattgga catgagccaa tataaatgta catattatga tatagataca acgtatgcaa
     1861 tggccaatag ccaatattga tttatgctat ataaccaatg aataatatgg ctaatggcca
     1921 atattgaaga tctcgatcct ctacgccgga cgcatcgtgg ccggagtact gcggtttatc
     1981 tcgatggcta cgagggcaga cagtaagtgg atttaccata atcccttaat tgtacgcacc
     2041 gctaaaacgc gttcagcgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc
     2101 gaaaaactgc cgcaatcgcg cctgataaat tttaaccgta tgaataccta tgcaaccaga
     2161 gggtacaggc cacattaccc ccacttaatc cactgaagct gccatttttc atggtttcac
     2221 catcccagcg aagggccatc cagcgtgcgt tcctgtattt ccggactata aaaggatcta
     2281 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2341 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgaaatcctt tttttctgcg
     2401 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2461 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2521 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2581 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2641 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2701 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2761 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2821 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2881 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2941 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3001 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3061 taaccgtatt accgcctttg agtgagctga taccgggtag aaagccagtc cgcagaaacg
     3121 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     3181 aaagaggatc cttatcgcta tcgattcaca caaaaaacca acacacagat gtaatgaaaa
     3241 taaagatatt ttattgcggc catcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
     3301 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     3361 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     3421 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     3481 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     3541 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     3601 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     3661 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     3721 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     3781 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     3841 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     3901 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     3961 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     4021 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     4081 agaacctgcg tgcaatccat cttgttcaat catgatctgc ctcctgggtt tagttcctca
     4141 ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtca acagttaatt
     4201 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     4261 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     4321 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     4381 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     4441 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     4501 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
     4561 tcagaaggta cctaaccaag ttcctctttc agaggttatt tcaggccatg gtgctgcgca
     4621 gatccgcgta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     4681 aaattgtaag cgttaatatt ttgttaaaat tcgattttac cacatttgta gaggttttac
     4741 ttgcttaaaa aaacctccca catctccccc tgaacctgaa acataaaatg aatgcaattg
     4801 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4861 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4921 atgtatctta tcatgtctgc tcgaagcggc cgccccaagg ggttatgcta gttattgctc
     4981 agcggtggca gcagccaact cagcttcctt tcgggctttg ttagcagccg gatcagcttg
     5041 catgcctgca ggtcgactct agaggatccc cgggtaccga gcccgaattc gtttaaacag
     5101 gctgggagga ggggtgcgcc gaatgttgcg aaggagtagg ctgcgaggtg gctgctgagg
     5161 ggaccgtggg ctgcatgggt ggcggaggtg gaggcggagg tggctggact ggggtctgtg
     5221 tgttgggaga tggaggcggc gtgggccgtt tgaatttgtc aggactgttg cttctccgtg
     5281 gtgaaggcct tgtgatacca tgggatgttc ccatgtgctg cacgtgaaga cccggggaat
     5341 tgtaataaga cattccagct ctagtcagtg aagttggtgg cgtgaaacca accgtgtgac
     5401 ttgcatatga tagaccaggt gatataggcc tgcttgaact cggagaaggt gtgtaaggga
     5461 tcgggctgga cacagtgcga ggtcttaatc cttgtgcaga catctcatta aaataccttt
     5521 cgtcgtccat ttctaagctg gactcactct cggagagctg tcgacagagg gcttctcttc
     5581 tctccatctc cctctgcagc ttcctctgga gcctcaagtt ctcttctctc atgtgacgtt
     5641 cctcctccag atactgtgcc attttctctg aatgctgtaa ctgagcagct ctcagttgct
     5701 tcttcagccg ttccacttca ttctttaaaa acctgatgtg acgcatcata ttttctggag
     5761 aatcaatctc catggagata tctctaggcg atggtggagc agagacgggc tggtctaatt
     5821 tttcctgcag gattcgcttt tcagcttcaa gcttatccat ccttttccag aggcgattaa
     5881 ctagtgcttc ttgttcttgt tccaatgtat tttcaaggtc aatcttctcc cgtctcaact
     5941 gttctaatgt aagttgctta gaaatggtgt cattctccag ttttttaatt ttcttcatca
     6001 gtttgttgac ctgaaattcc tgctcttgtt caagatgctg ttctagttcg gctttctcat
     6061 gctgcaactg catcaatttt ctggagagct cattagtgag gaattcttct tctttctcat
     6121 aatttacagc aagggtttct ttctccttct gcaaagcctg aattttcttg aataaagtgt
     6181 tactaatgaa t
//
