LOCUS       sequence-472606-        9466 bp    DNA     circular SYN 05-SEP-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3145
FEATURES             Location/Qualifiers
     source          1..9466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    719..844
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             891..1071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1072..1298)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1326..1346)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1326..1344)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1365..1381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1365..1381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1378..1400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1389..1405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1413..1419,1420..1437,1438..1443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1458..1479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1596..1613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1767..2355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1847..1866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2526..3317,3318..3386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3149..3168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3387..3491)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3517..3972)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3654..3675)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3866..3885
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4099..4121
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4113..4130
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4114..4130
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4140..4159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4140..4158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4179..4314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4354..4475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4365..4384)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4419..4438
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4553..4786
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4858..5446
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4964..4975
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5373..5393
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5557..5598)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5606..5630
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6627..6651)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6671..7849)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6680..7618
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6703..6723)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7987..8104
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8147..8749)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8233..8253)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8730..8749
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8781..8977)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(8789..8999)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8795..8930
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(9230..9274)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9459..9466,1..226)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
       61 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
      121 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
      181 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
      241 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
      301 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc
      361 aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct
      421 ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga
      481 gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt
      541 gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg
      601 cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc
      661 cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga
      721 cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca
      781 ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag
      841 agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat
      901 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      961 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     1021 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cgtttatgta
     1081 tcgagctagg cacttaaata caatatctct gcaatgcggc aattcagtgg ttcgtccaat
     1141 ccatgtcaga cccgtctgtt gccttcctaa taaggcacga tcgtaccacc ttacttccac
     1201 caatcggcat gcacggtgct ttttctctcc ttgtaaggca tgttgctaac tcatcgttac
     1261 catgttgcaa gactacaaga gtattgcata agactacatt aagcttgcag ctccagcttt
     1321 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     1381 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1441 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1501 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1561 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1621 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1681 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2401 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2461 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2521 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2581 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2641 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2701 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2761 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2821 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2881 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2941 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3001 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3061 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3121 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3181 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3241 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3301 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3361 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3421 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3481 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     3541 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     3601 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     3661 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3721 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3781 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     3841 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3901 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3961 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     4021 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     4081 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     4141 aatacgactc actatagggc gaattgggta cgtccctagg cctccaaaaa agcctcctca
     4201 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     4261 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     4321 agttaggggc gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa
     4381 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     4441 tatttgtaac cattataagc tgcaataaac aagttcctct cactctctga tattcatttc
     4501 tttgcaagtt ataaatactg aataataaga tgacatgaac tactatacgt actgctagag
     4561 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     4621 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     4681 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa
     4741 gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     4801 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa agtcgacgcg
     4861 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     4921 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     4981 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaaggaa
     5041 aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     5101 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     5161 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     5221 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     5281 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     5341 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     5401 gaataccagt caatctttca caaattttgt aatccagagg ttgattgtcg acttaacgcg
     5461 ttggaacgtc ggtgcataag gtggatcgtc ctttccacaa gatatataaa gccaagaaat
     5521 cgaaatactt tcaagttacg gactagttca ttactacgta gaatcgagac cgaggagagg
     5581 gttagggata ggcttaccga tatcaaccac tttgtacaag aaagttggca atgaggtttc
     5641 tttctcctca ttatgtttcc tctggtgttg aataagctgt gaactatacc gataggcttt
     5701 cccacactca ctacatttat agggtttctc cttagtgtgg gttctcaagt gtagaataac
     5761 ttgagaagtc tgcctgaagg ttttcccaca ttcattacat ttatagggtt tctctccagt
     5821 gtgaactctc tgatgatcaa caaggtttcg attagaagta aaagccttcc cacactcatt
     5881 acatttgtaa ggtttctctc tacgatgaag tctctggtgt tcattaagag ttttccttaa
     5941 gatgaaagtt tttccacact catcacattc ataaggcttc tctccagtgt gaattctctg
     6001 atggtccata atctttgtat tagaaccaca tgttttccca cactccttac atttgtaaac
     6061 tttttttcct ctgtgcattc gctgatgttg agtaaggttt gaactacggc taaaggcttt
     6121 cccacactca atacaagaat agggcttctc tccggtgtga actctgtgat gtgatataag
     6181 gcccgaactc cgactaaagg ctttcccaca ctctttacat tcataagttt tctccccact
     6241 gtggattccc tggtgccgaa taagatgtga ctttccactg aaagatttcc cacattcact
     6301 gcatgtatag ggcttcagtc cagtgtggat tctccgatga acaacaaggt tagagctatg
     6361 actgaaagct tttccacact ccttacactt atagggtttc tctcccgtgt ggattcgctc
     6421 atgtactgtg aggtttgcac tctgactaaa ggctttccca cactcagtac atacatactg
     6481 tctcttttca gcctgaactc tctcatttgt tgcagggtca cagcactgac tattgtcttt
     6541 ttcagattct tggtcactcc cttctgtgag tactttattg tctttaactg tcttatgttt
     6601 gaaatttctt gtctttctcc tcatggtgcc aacttttttg tacaaacttg ttgatcaatc
     6661 gatgctagcc tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct
     6721 tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt
     6781 gccaagctgg cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa
     6841 acccctcccc ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc
     6901 tggacggcgc ccggtactcc gtggagtcac atgaagcgac ggctgaggac ggaaaggccc
     6961 ttttcctttg tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc ctccattttg
     7021 agctccctgc agcagggccg ggaagcggcc atctttccgc tcacgcaact ggtgccgacc
     7081 gggccagcct tgccgcccag ggcggggcga tacacggcgg cgcgaggcca ggcaccagag
     7141 caggccggcc agcttgagac tacccccgtc cgattctcgg tggccgcgct cgcaggcccc
     7201 gcctcgccga acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa
     7261 ccgaaatacc agtgtgcaga tcttggcccg catttacaag actatcttgc cagaaaaaaa
     7321 gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga cttatcgaaa gcagcgagac
     7381 aggcgcgaag gtgccaccag attcgcacgc ggcggcccca gcgcccaggc caggcctcaa
     7441 ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct cgaactctcc cacccacttc
     7501 caacccgaag ctcgggatca agaatcacgt actgcagcca ggtggaagta attcaaggca
     7561 cgcaagggcc ataacccgta aagaggccag gcccgcggga accacacacg gcacttacct
     7621 gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat
     7681 acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt
     7741 ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct
     7801 cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcca attcccactc
     7861 ctttcaagac ctagaaggtc cattagctgc aaagattcct ctctgtttaa aactttatcc
     7921 atctttgcac ctgcaggcct cgagccggcg ccaaagtgga tctctgctgt ccctgtaata
     7981 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
     8041 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     8101 aaaattgtgg atgaatactg ccatttgtct caagatctag aattcgttaa ctggcaccgg
     8161 gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg
     8221 tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg
     8281 gcaccccggc gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct
     8341 tgccctggtg gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc tccttgggcc
     8401 ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact
     8461 cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg
     8521 tccagaccgc cacggcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga
     8581 cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccggatcga
     8641 cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggccctgg
     8701 ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gtaagctccg
     8761 gtggtacctc tagaaagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     8821 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     8881 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     8941 aggggcggga ctatggttgc tgactaattg agacgcatgc tttgcatact tctgcctgct
     9001 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcaa tgcatgggtc
     9061 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
     9121 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
     9181 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
     9241 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
     9301 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
     9361 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
     9421 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctg
//
