LOCUS       sequence-472607-        9628 bp    DNA     circular SYN 05-SEP-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3133
FEATURES             Location/Qualifiers
     source          1..9628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9..25)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18..40)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      167..622
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(254..273)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     464..485
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        648..752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(753..821,822..1613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(971..990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1784..2372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2273..2292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2526..2543
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2660..2681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2696..2701,2702..2719,2720..2726)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2734..2750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2739..2761
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2758..2774
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2758..2774
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2793..2813
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2795..2813
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2841..3067
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3068..3248
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3295..3420
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3913..4146
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4331..4375
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4606..4816
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4628..4824
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4675..4810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4856..5458
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(4856..4875)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5352..5372
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5501..5618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5756..6934
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5987..6925
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6882..6902
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6954..6978
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8137..8161)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8169..8210
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8321..8909
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8374..8394)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8792..8803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8981..9214
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9292..9413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9329..9348)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9383..9402
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9453..9588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9608..9627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9609..9627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
      181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      241 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      361 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      481 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      541 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
      601 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      661 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      721 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      781 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      841 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      901 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      961 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1021 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1081 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1141 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1201 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1261 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1321 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1381 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1441 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1501 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1561 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1621 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1681 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1921 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2101 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2161 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2221 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2281 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2341 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2401 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2461 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2521 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2581 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2641 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2701 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2761 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     2821 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     2881 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     2941 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     3001 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     3061 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3121 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3181 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3241 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     3301 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     3361 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     3421 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3481 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3541 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3601 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3661 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3721 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3781 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3841 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3901 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3961 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     4021 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     4081 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     4141 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     4201 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     4261 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     4321 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     4381 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     4441 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4501 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     4561 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4621 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4681 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4741 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4801 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     4861 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     4921 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4981 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5041 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     5101 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     5161 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     5221 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     5281 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     5341 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     5401 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
     5461 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     5521 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5581 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     5641 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
     5701 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
     5761 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5821 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5881 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5941 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     6001 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     6061 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     6121 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     6181 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     6241 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     6301 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     6361 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     6421 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     6481 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     6541 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     6601 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     6661 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     6721 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     6781 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     6841 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     6901 tcaaagtttt tttcttccat ttcaggtgtc gtgaggctag catcgattga tcaacaagtt
     6961 tgtacaaaaa agcaggcacc atgccgcagc tggactccgg cgggggcggc gcgggcggcg
     7021 gcgacgacct cggcgcgccg gacgagctgc tggccttcca ggatgaaggc gaggagcagg
     7081 acgacaagag ccgcgacagc gccgccggtc ccgagcgcga cctggccgag ctcaagtcgt
     7141 cgctcgtgaa cgagtccgag ggcgcggccg gcggcgcagg gatcccgggg gtcccggggg
     7201 ccggcgccgg ggcccgcggc gaggccgagg ctctcgggcg ggaacacgct gcgcagagac
     7261 tcttcccgga caaacttcca gagcccctgg aggacggcct gaaggccccg gagtgcacca
     7321 gcggcatgta caaagagacc gtctactccg ccttcaatct gctcatgcat tacccacccc
     7381 cctcgggagc agggcagcac ccccagccgc agcccccgct gcacaaggcc aatcagcccc
     7441 cccacggtgt cccccaactc tctctctacg aacatttcaa cagcccacat cccacccctg
     7501 cacctgcgga catcagccag aagcaagttc acaggcctct gcagacccct gacctctctg
     7561 gcttctactc cctgacctca ggcagcatgg ggcagctccc ccacactgtg agctggttca
     7621 cccacccatc cttgatgcta ggttctggtg tacctggtca cccagcagcc atcccccacc
     7681 cggccattgt gcccccctca gggaagcagg agctgcagcc cttcgaccgc aacctgaaga
     7741 cacaagcaga gtccaaggca gagaaggagg ccaagaagcc aaccatcaag aagcccctca
     7801 atgccttcat gctgtacatg aaggagatga gagccaaggt cattgcagag tgcacactta
     7861 aggagagcgc tgccatcaac cagatcctgg gccgcaggtg gcacgcgctg tcgcgagaag
     7921 agcaggccaa gtactatgag ctggcccgca aggagaggca gctgcacatg cagctatacc
     7981 caggctggtc agcgcgggac aactacggga agaagaagag gcggtcgagg gaaaagcacc
     8041 aagaatccac cacaggagga aaaagaaatg cattcggtac ttacccggag aaggccgctg
     8101 ccccagcccc gttccttccg atgacagtgc tctagaaccc agctttcttg tacaaagtgg
     8161 ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgaa
     8221 ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     8281 atttgaatat cgggtttttt ccaatacgcg ttaagtcgac aatcaacctc tggattacaa
     8341 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     8401 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     8461 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     8521 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     8581 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     8641 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     8701 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     8761 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     8821 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     8881 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     8941 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9001 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9061 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9121 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9181 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     9241 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     9301 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9361 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9421 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     9481 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9541 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     9601 caattcgccc tatagtgagt cgtattac
//
