LOCUS       sequence-472608-       10201 bp    DNA     circular SYN 05-SEP-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3134
FEATURES             Location/Qualifiers
     source          1..10201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..61
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      112..247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(267..286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(268..286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(296..313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(296..312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(305..327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      454..909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(541..560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     751..772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        935..1039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1040..1108,1109..1900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1258..1277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2071..2659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2560..2579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2813..2830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2947..2968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2983..2988,2989..3006,3007..3013)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3021..3037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3026..3048
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3045..3061
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3045..3061
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3080..3100
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3082..3100
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3128..3354
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3355..3535
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3582..3707
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4200..4433
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4618..4662
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4893..5103
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4915..5111
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4962..5097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5143..5745
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(5143..5162)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5639..5659
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5788..5905
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6043..7221
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6274..7212
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7169..7189
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7241..7265
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8997..9021)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9029..9070
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    9181..9769
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9234..9254)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9652..9663)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9841..10074
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    join(10152..10201,1..72)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(join(10189..10201,1..7))
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
       61 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
      121 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      181 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      241 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
      301 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      361 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      421 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      661 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      781 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      901 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1021 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1081 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1141 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1201 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1261 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1321 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1381 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1441 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1501 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1561 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1621 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1681 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1741 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1801 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1861 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1921 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1981 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2041 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2101 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2161 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2221 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2281 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2341 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2401 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2461 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2521 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2581 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2641 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2701 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2761 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2821 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2881 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2941 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3001 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     3061 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     3121 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     3181 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     3241 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     3301 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     3361 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3421 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3481 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     3541 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     3601 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     3661 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     3721 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     3781 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     3841 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     3901 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     3961 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     4021 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     4081 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     4141 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     4201 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     4261 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     4321 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     4381 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     4441 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     4501 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     4561 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     4621 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     4681 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     4741 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     4801 tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc aattagtcag
     4861 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc
     4921 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4981 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5041 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5101 gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga gtacaagccc
     5161 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5221 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5281 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5341 gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5401 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5461 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5521 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5581 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5641 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5701 ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat tctagatctt
     5761 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     5821 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     5881 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     5941 gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga gaggaatctt
     6001 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     6061 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6121 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6181 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6241 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     6301 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     6361 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     6421 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     6481 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     6541 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     6601 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6661 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6721 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6781 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6841 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6901 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6961 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     7021 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     7081 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     7141 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     7201 cttccatttc aggtgtcgtg aggctagcat cgattgatca acaagtttgt acaaaaaagc
     7261 aggcaccatg tctcatgcgg ccgaaccagc tcgggatggc gtagaggcca gcgcggaggg
     7321 ccctcgagcc gtgttcgtgc tgttggagga gcgcaggccg gccgactcgg ctcagctgct
     7381 cagcctgaac tctttgcttc cggaatccgg gattgttgct gacatagaat tagaaaacgt
     7441 ccttgatcct gacagcttct acgagctcaa aagccaaccc ttaccccttc gctcaagcct
     7501 cccaatatca ctgcaggcca caccagccac cccagctaca ctctctgcat cgtcttctgc
     7561 agggggctcc aggacccctg ccatgtcgtc atcttcttca tcgagggtct tgctgcggca
     7621 gcagctaatg cgggcccagg cgcaggagca ggagaggcgt gagcgtcggg aacaggccgc
     7681 cgcggctccc ttccccagtc ctgcacctgc ctctcctgcc atctctgtgg ttggcgtctc
     7741 tgctgggggc cacacattga gccgtccacc ccctgctcag gtgcccaggg aggtgctcaa
     7801 ggtgcagacc catctggaga acccaacgcg ctaccacctg cagcaggcgc gccggcagca
     7861 ggtgaaacag tacctgtcca ccacactcgg gcccaagctg gcttcccagg ccctcacccc
     7921 accgccgggg cccgcaagtg cccagccact gcctgcccct gaggctgccc acactaccgg
     7981 ccccacaggc agtgcgccca acagccccat ggcgctgctc accatcgggt ccagctcaga
     8041 gaaggagatt gatgatgtca ttgatgagat catcagcctg gagtccagtt acaatgatga
     8101 aatgctcagc tatctgcccg gaggcaccac aggactgcag ctccccagca cgctgcctgt
     8161 gtcagggaat ctgcttgatg tgtacagtag tcaaggcgtg gccacaccag ccatcactgt
     8221 cagcaactcc tgcccagctg agctgcccaa catcaaacgg gagatctctg agaccgaggc
     8281 aaaggccctt ttgaaggaac ggcagaagaa agacaatcac aacctaattg agcgtcgcag
     8341 gcgattcaac attaacgaca ggatcaagga actgggcact ctcatcccta agtccagtga
     8401 cccggagatg cgctggaaca agggcaccat cctgaaggcc tctgtggatt atatccgcaa
     8461 gctgcagaag gagcagcagc gctccaaaga cctggagagc cggcagcgat ccctggagca
     8521 ggccaaccgc agcctgcagc tccgaattca ggaactagaa ctgcaggccc agatccatgg
     8581 cctgccagtg cctcccactc cagggctgct ttccttggcc acgacttcgg cttctgacag
     8641 cctcaagcca gagcagctgg acattgagga ggagggcagg ccaggcgcag caacgttcca
     8701 tgtagggggg ggacctgccc agaatgctcc ccatcagcag ccccctgcac cgccctcaga
     8761 tgcccttctg gacctgcact ttcccagcga ccacctgggg gacctgggag accccttcca
     8821 cctggggctg gaggacattc tgatggagga ggaggagggg gtggtgggag gactgtcggg
     8881 gggtgccctg tccccactgc gggctgcctc cgatcccctg ctctcttcag tgtcccctgc
     8941 tgtctccaag gccagcagcc gccgcagcag cttcagcatg gaagaggagt cctagaaccc
     9001 agctttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
     9061 cgattctacg tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     9121 tatatcttgt ggaaaggacg aaataatatc atatcaacgc cattcacgcg ttaagtcgac
     9181 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9241 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9301 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9361 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9421 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     9481 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9541 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     9601 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9661 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9721 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     9781 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     9841 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     9901 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9961 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10021 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
    10081 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    10141 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10201 c
//
