LOCUS       sequence-472623-       10220 bp    DNA     circular SYN 14-MAY-2025
DEFINITION  Protein expression of ScVPS30 and ScVPS38.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, 
            Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL
  TITLE     Structure and flexibility of the endosomal Vps34 complex reveals the
            basis of its function on membranes.
  JOURNAL   Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.
  PUBMED    26450213
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2015-10-9"; volume: "350(6257):aac7365. doi"; pages: " 
            10.1126/science.aac7365"
COMMENT     Sequence Label: pYO539
FEATURES             Location/Qualifiers
     source          1..10220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2070..2187
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    4178..4295
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(6618..6638)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6618..6636)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6657..6673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6657..6673)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6670..6692)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6681..6697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6705..6711,6712..6729,6730..6735))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6750..6771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6888..6905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7059..7647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7139..7158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7818..8609,8610..8678))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8441..8460
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8679..8783)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8810..10152
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9773..9820)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10193..10211
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgc tatgacatga ttacgaattg gcgcgccggc cggccggatc
     2041 tttgcaaaga tccaaaaaaa gcatgcgtac ggattagaag ccgccgagcg ggtgacagcc
     2101 ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc
     2161 agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg
     2221 gttatgaaga ggaaaattgg cagtaacctg gccccacaaa cctcaaatga acgaatcaaa
     2281 ttaacaacca gatatctcga ctgaaaaaaa aggtttaaac cagttccctg aaattattcc
     2341 cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct gtaaatctat
     2401 ttcttaaact tcttgaattc tacttttata gttagtcttt tttttagttt taaaacacca
     2461 agaacttagt ttcgaataaa cacacataaa taaaccatgg gcatgaaacg gttcttactc
     2521 agtcgaagac agagacacct tcgtatgatt tgctttcaca atataagtct ctttagagct
     2581 aatggtgact ccaaattgat caaggaatat ggtgatggat ttataccatg tttctttatc
     2641 ttggaatcca taagagggga gctgctttat gtgagcgagg tacaatcagg ttctttgcgt
     2701 aaactctctt ttcaggaatt accaaaacta actggtgcat ctactatgat tgtcctaaaa
     2761 ttggtaggac tagttccgag tgatattttg tgtaccataa gttcagataa aaatggtatc
     2821 attgacgata aatggtgcgt tttatgtacc tacacgatag atcttaacaa gttacaaccc
     2881 ataaatgaag acacagttct aattacaggc accaacgcac cagtacttga tttaatagat
     2941 ggatcctaca cattggctgc agaaaaaatt aagccactaa agggccttgt tagttctcac
     3001 aaaaggaata taagtcaagt caagatcaaa ttttcattgg catatagctc tttactaaaa
     3061 ttaaacaaac tattggagta ttcttcccaa gtccatgagg aaatcaatga aatttccagc
     3121 aaaatagaag atgacttttt gtcactcaag aatcaaaatc attggtatat gagaacagta
     3181 cagaagtcca tagaaacgct agaaaaggaa gtcctacaaa ggaaaaaaag caagaaaaat
     3241 atagaaatgg cgcaactcga gagtaatgat actatcaacc attctaaaac agaactgtct
     3301 ctgatgtcgc aggacgagtc tataaatgat gactatggaa gcatatattc tagatttgtg
     3361 caaataaaag ataggttgga ccaactgagg tttaagaaac tgtatcagct gatagggatc
     3421 ttccattcaa cggatttatt taattcagat agaggctata tttactttga aaaaccttct
     3481 agcgtaaatg atgttattaa taggctcaag ctaaaacctt tgaatataga gatattactt
     3541 agacaagccg gcgaatccac aaagcacaga gaatatgtaa acagccagtt gggttattac
     3601 cttttatttc tccatttgac agccatccaa attttcaagg ctcctttacc ctatagacta
     3661 atgtattatg gtagtacatc agtgatcgac agtcaatatc cgttgtattt cactgatcaa
     3721 atgatttcta agcatcaagc aaagctgatt aaagccatac attattttaa tgctgatata
     3781 ttgcaattta aacagattct ggagaattac cgtccaacag gatcctaacc caagcttaag
     3841 tcgactttgt tcccactgta cttttagctc gtacaaaata caatatactt ttcatttctc
     3901 cgtaaacaac atgttttccc atgtaatatc cttttctatt tttcgttccg ttaccaactt
     3961 tacacatact ttatatagct attcacttct atacactaaa aaactaagac aattttaatt
     4021 ttgctgcctg ccatatttca atttgttata aattcctata atttatccta ttagtagcta
     4081 aaaaaagatg aatgtgaatc gaatcctaag agaattcccc tcgaagatcg atctggcggc
     4141 cgcggatctt tgcaaagatc caaaaaaagc atgcgtacgg attagaagcc gccgagcggg
     4201 tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc
     4261 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag
     4321 cttttatggt tatgaagagg aaaattggca gtaacctggc cccacaaacc tcaaatgaac
     4381 gaatcaaatt aacaaccaga tatctcgact gaaaaaaaag gtttaaacca gttccctgaa
     4441 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
     4501 aaatctattt cttaaacttc ttgaattcta cttttatagt tagtcttttt tttagtttta
     4561 aaacaccaag aacttagttt cgaataaaca cacataaata aaccatgggc atgaagtgcc
     4621 aaacatgtca cctgccgctg caactggacc catctttaga aggtcttagt ttaacccaaa
     4681 gaaatttgct actttcaaat aattcaatta tcactgcaac gaatgaaaat gtcatcagca
     4741 acaagggcat cgaagccgca gataattgtg ggcctcaaat accgaaggag aggctgaggc
     4801 gattagggga gattcagaac ataaaagact tgaatttaat agatgacaaa ttgatcactg
     4861 attcgtttgt ttttcttaat cacgatgatg atgataatgc taatattacg agtaatagca
     4921 gagaggacca gcgttatgga aatgccaacg gaaatgataa taaaaaagct aatagtgata
     4981 cttccgatgg aaccagtacc tttcgagatc atgacgaaga ggaacaagaa gctacggatg
     5041 aagacgaaaa ccaacaaata caattgaatt ccaagacttt gtccacacaa gtcaacgcaa
     5101 tgactaatgt atttaacatt ctatcttctc aaacaaacat agactttcct atctgtcagg
     5161 actgctgtaa catcttgata aacagactaa agagtgagta tgatgatgca ataaaagaga
     5221 gagatactta cgctcaattt ttatcgaagt tagagagtca aaacaaagaa atatcagaaa
     5281 gcaataaaga aaagcagtat tcccataatc tttcggaaaa agaaaatttg aaaaaggaag
     5341 aggaaagact cttggatcaa ttattacgtt tggaaatgac agatgatgac ttagatggag
     5401 aactagttcg tttacaagaa aagaaagttc aactggaaaa tgagaaactc caaaaactga
     5461 gtgatcagaa tttaatggat ttaaataaca ttcaattcaa caaaaattta cagtcattaa
     5521 aactgcaata cgaattatcc ttgaatcaat tagacaaatt gaggaaaatc aatattttca
     5581 atgctacatt caaaatctca catagcggcc cctttgccac tataaatgga ttgagattag
     5641 gcagtatacc agaatcagtg gttccttgga aggaaatcaa tgcagcactg ggccaattga
     5701 ttcttttact ggctaccata aacaaaaacc tgaagataaa tttagtggac tacgaattac
     5761 aaccgatggg ttcattttcc aaaatcaaga aaagaatggt taacagtgtc gaatataata
     5821 attccactac gaacgctccc ggtgattggt tgattctgcc tgtctactat gatgagaatt
     5881 tcaatttggg gagaatcttc cgcaaggaaa cgaagtttga taaatcatta gaaacaacat
     5941 tagaaataat aagcgaaatc actcggcagc tttctacgat agcgtcatca tattcatctc
     6001 aaacactcgc aacaagccaa gatgaatctt caatgaataa cgcaaatgac gttgaaaata
     6061 gtacttccat tctcgagcta ccctatatca tgaataagga caagattaat ggattatccg
     6121 tcaaattgca tggtagcagt cccaaccttg aatggacaac ggctatgaaa tttttattaa
     6181 ccaatgtaaa gtggttgtta gcgttttctt ccaatttact atccaagtca attaccttaa
     6241 gccctacagt gaatcacaat gataagacca tcagcggaaa cggatcctaa cccaagctta
     6301 agtcgacttt gttcccactg tacttttagc tcgtacaaaa tacaatatac ttttcatttc
     6361 tccgtaaaca acatgttttc ccatgtaata tccttttcta tttttcgttc cgttaccaac
     6421 tttacacata ctttatatag ctattcactt ctatacacta aaaaactaag acaattttaa
     6481 ttttgctgcc tgccatattt caatttgtta taaattccta taatttatcc tattagtagc
     6541 taaaaaaaga tgaatgtgaa tcgaatccta agagaattcc cctcgaagat cgatctggcg
     6601 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     6661 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6721 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6781 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6841 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6961 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7021 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7081 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7141 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7201 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7261 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7321 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7381 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7441 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7501 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7561 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7621 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7741 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7801 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7861 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7921 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7981 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8041 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8101 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8161 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8221 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8281 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8341 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8401 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8461 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8521 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8581 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8641 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8701 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8761 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc
     8821 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     8881 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
     8941 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     9001 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
     9061 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
     9121 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
     9181 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
     9241 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
     9301 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
     9361 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
     9421 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
     9481 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
     9541 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
     9601 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
     9661 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
     9721 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
     9781 tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
     9841 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
     9901 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
     9961 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
    10021 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
    10081 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
    10141 tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10201 tatcacgagg ccctttcgtc
//
